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anti-ADA antibody :: Rabbit anti-Human ADA Polyclonal Antibody

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Catalog # MBS9210674
Unit / Price
  0.08 mL  /  $165 +1 FREE 8GB USB
  0.4 mL  /  $370 +1 FREE 8GB USB
Product Name

ADA, Polyclonal Antibody

Popular Item
Full Product Name

ADA Antibody (C-term)

Product Synonym Names
Adenosine deaminase; Adenosine aminohydrolase; ADA; ADA1
Product Gene Name
Antibody/Peptide Pairs
ADA peptide (MBS9218533) is used for blocking the activity of ADA antibody (MBS9210674)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Positions
287-314
OMIM
102700
3D Structure
ModBase 3D Structure for P00813
Clonality
Polyclonal
Isotype
Rabbit Ig
Host
Rabbit
Species Reactivity
Human
Specificity
This ADA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 287-314 amino acids from the C-terminal region of human ADA.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Antigen Type
Synthetic Peptide
Crown Antibody
Yes
Antigen Source
HUMAN
Function
Catalyzes the hydrolytic deamination of adenosine and 2- deoxyadenosine. Plays an important role in purine metabolism and in adenosine homeostasis. Modulates signaling by extracellular adenosine, and so contributes indirectly to cellular signaling events. Acts as a positive regulator of T-cell coactivation, by binding DPP4. Its interaction with DPP4 regulates lymphocyte- epithelial cell adhesion.
Cellular Location
Cell membrane; Peripheral membrane protein; Extracellular side. Cell junction. Cytoplasmic vesicle lumen. Cytoplasm. Note=Colocalized with DPP4 at the cell junction in lymphocyte-epithelial cell adhesion
Tissue Location
Found in all tissues, occurs in large amounts in T-lymphocytes and, at the time of weaning, in gastrointestinal tissues
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Other Notes
Small volumes of anti-ADA antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-ADA antibody
This gene encodes an enzyme that catalyzes the hydrolysis
of adenosine to inosine. Various mutations have been described for
this gene and have been linked to human diseases. Deficiency in
this enzyme causes a form of severe combined immunodeficiency
disease (SCID), in which there is dysfunction of both B and T
lymphocytes with impaired cellular immunity and decreased
production of immunoglobulins, whereas elevated levels of this
enzyme have been associated with congenital hemolytic anemia.
Applications Tested/Suitable for anti-ADA antibody
Flow Cytometry (FC/FACS), Western Blot (WB)
Application Notes for anti-ADA antibody
WB~~1:1000
FC~~1:10~50

Western Blot (WB) of anti-ADA antibody
ADA Antibody (C-term) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the ADA antibody detected the ADA protein (arrow).
anti-ADA antibody Western Blot (WB) (WB) image
Flow Cytometry (FC/FACS) of anti-ADA antibody
ADA Antibody (C-term) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
anti-ADA antibody Flow Cytometry (FC/FACS) (FC/FACS) image
NCBI/Uniprot data below describe general gene information for ADA. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
40764
NCBI Official Full Name
adenosine deaminase
NCBI Official Synonym Full Names
adenosine deaminase
NCBI Official Symbol
NCBI Protein Information
adenosine deaminase
UniProt Protein Name
Adenosine deaminase
UniProt Synonym Protein Names
Adenosine aminohydrolase
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
UniProt Entry Name
ADA_HUMAN
NCBI Summary for ADA
This gene encodes an enzyme that catalyzes the hydrolysis of adenosine to inosine. Various mutations have been described for this gene and have been linked to human diseases. Deficiency in this enzyme causes a form of severe combined immunodeficiency disease (SCID), in which there is dysfunction of both B and T lymphocytes with impaired cellular immunity and decreased production of immunoglobulins, whereas elevated levels of this enzyme have been associated with congenital hemolytic anemia. [provided by RefSeq, Jul 2008]
UniProt Comments for ADA
ADA: a enzyme that converts adenosine + H2O into inosine + NH3. Found in all tissues, occurs in large amounts in T-lymphocytes and, at the time of weaning, in gastrointestinal tissues. Genetic ADA deficiencies are a cause of autosomal recessive severe combined immuno-deficiency (SCID). Hereditary hemolytic anemia is caused by expression levels in erythrocytes 50-70 times greater than the norm.

Protein type: Nucleotide Metabolism - purine; EC 3.5.4.4; Hydrolase

Chromosomal Location of Human Ortholog: 20q13.12

Cellular Component: dendrite cytoplasm; extracellular space; cell surface; cell soma; membrane; lysosome; cytoplasm; plasma membrane; cell junction; cytosol; external side of plasma membrane

Molecular Function: adenosine deaminase activity; protein binding; zinc ion binding; purine nucleoside binding

Biological Process: negative regulation of circadian sleep/wake cycle, non-REM sleep; T cell activation; adenosine catabolic process; deoxyadenosine catabolic process; response to morphine; positive regulation of calcium-mediated signaling; purine salvage; histamine secretion; positive regulation of T cell differentiation in the thymus; purine ribonucleoside monophosphate biosynthetic process; response to vitamin E; regulation of cell-cell adhesion mediated by integrin; positive regulation of T cell receptor signaling pathway; negative regulation of mature B cell apoptosis; positive regulation of germinal center formation; positive regulation of B cell proliferation; hypoxanthine salvage; negative regulation of adenosine receptor signaling pathway; positive regulation of smooth muscle contraction; embryonic gut development; placenta development; aging; Peyer's patch development; response to drug; nucleobase, nucleoside and nucleotide metabolic process; dATP catabolic process; positive regulation of heart rate; liver development; negative regulation of leukocyte migration; purine base metabolic process; trophectodermal cell differentiation; purine nucleotide salvage; xanthine biosynthetic process; response to hydrogen peroxide; negative regulation of inflammatory response; response to hypoxia; inosine biosynthetic process; germinal center B cell differentiation; alveolus development; positive regulation of alpha-beta T cell differentiation

Disease: Severe Combined Immunodeficiency, Autosomal Recessive, T Cell-negative, B Cell-negative, Nk Cell-negative, Due To Adenosine Deaminase Deficiency
Product References and Citations for anti-ADA antibody
Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Gloria-Bottini, F., et al. Am. J. Med. Sci. 340(2):103-108(2010)
Levine, A.J., et al. Cancer Epidemiol. Biomarkers Prev. 19(7):1812-1821(2010)
Spina, C., et al. Cancer Invest. (2010) In press :
Ri, G., et al. Anticancer Res. 30(6):2347-2349(2010)

Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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