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anti-ADAMTS12 antibody :: Rabbit anti-Human ADAMTS12 Polyclonal Antibody

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Catalog # MBS9609487
Unit / Price
  0.1 mL  /  $255 +1 FREE 8GB USB
  0.2 mL  /  $295 +1 FREE 8GB USB
Western Blot (WB)
Product Name

ADAMTS12, Polyclonal Antibody

Popular Item
Also Known As

ADAMTS12 Antibody

Product Synonym Names
A disintegrin and metalloproteinase with thrombospondin motifs 12; A disintegrin like and metalloprotease with thrombospondin type 1 motif 12; ADAM metallopeptidase with thrombospondin type 1 motif 12; ADAM TS 12
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
232
3D Structure
ModBase 3D Structure for P58397
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Specificity
ADAMTS12 antibody detects endogenous levels of total ADAMTS12
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (lot specific)
Immunogen
A synthesized peptide derived from human ADAMTS12
Subcellular Location
Secreted
Tissue Specificity
Expressed in skeletal muscle and fat. Detected at significant levels in fetal lung. Widely expressed in gastric carcinomas and in cancer cells of diverse origin.
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.
Other Notes
Small volumes of anti-ADAMTS12 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-ADAMTS12 antibody
Function: Metalloprotease that may play a role in the degradation of COMP. Cleaves also alpha-2 macroglobulin and aggregan. Has anti-tumorigenic properties.
Subunit Structure: Interacts with COMP.
Post-translational Modifications: The precursor is cleaved by a furin endopeptidase. Subjected to an intracellular maturation process yielding a 120 kDa N-terminal fragment containing the metalloproteinase, disintegrin, one TSP type-1 and the Cys-rich domains and a 83 kDa C-terminal fragment containing the spacer 2 and four TSP type-1 domains. Glycosylated. Can be O-fucosylated by POFUT2 on a serine or a threonine residue found within the consensus sequence C1-X2-(S/T)-C2-G of the TSP type-1 repeat domains where C1 and C2 are the first and second cysteine residue of the repeat, respectively. Fucosylated repeats can then be further glycosylated by the addition of a beta-1, 3-glucose residue by the glucosyltransferase, B3GALTL. Fucosylation mediates the efficient secretion of ADAMTS family members. Also can be C-glycosylated with one or two mannose molecules on tryptophan residues within the consensus sequence W-X-X-W of the TPRs, and N-glycosylated. These other glycosylations can also facilitate secretion (By similarity).
Similarity: The spacer domain and the TSP type-1 domains are important for a tight interaction with the extracellular matrix.The C-terminal four TSP1-like repeats are necessary and sufficient for binding COMP.The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
Applications Tested/Suitable for anti-ADAMTS12 antibody
Western Blot (WB), ELISA (EIA)
Application Notes for anti-ADAMTS12 antibody
WB: 1:1000-3000

Western Blot (WB) of anti-ADAMTS12 antibody
Western blot analysis of ADAMTS12 using HuvEc whole cell lysates
anti-ADAMTS12 antibody Western Blot (WB) (WB) image
NCBI/Uniprot data below describe general gene information for ADAMTS12. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
Observed: 178 kDa
Predicted: 178 kDa
NCBI Official Full Name
A disintegrin and metalloproteinase with thrombospondin motifs 12 isoform 2
NCBI Official Synonym Full Names
ADAM metallopeptidase with thrombospondin type 1 motif 12
NCBI Official Symbol
ADAMTS12  [Similar Products]
NCBI Official Synonym Symbols
PRO4389
  [Similar Products]
NCBI Protein Information
A disintegrin and metalloproteinase with thrombospondin motifs 12
UniProt Protein Name
A disintegrin and metalloproteinase with thrombospondin motifs 12
UniProt Gene Name
ADAMTS12  [Similar Products]
UniProt Synonym Gene Names
ADAM-TS 12; ADAM-TS12; ADAMTS-12  [Similar Products]
NCBI Summary for ADAMTS12
This gene encodes a member of the ADAMTS (a disintegrin and metalloproteinase with thrombospondin motifs) protein family. Members of the family share several distinct protein modules, including a propeptide region, a metalloproteinase domain, a disintegrin-like domain, and a thrombospondin type 1 (TS-1) motif. Individual members of this family differ in the number of C-terminal TS-1 motifs, and some have unique C-terminal domains. The enzyme encoded by this gene contains eight TS-1 motifs. It may play roles in pulmonary cells during fetal development or in tumor processes through its proteolytic activity or as a molecule potentially involved in regulation of cell adhesion. [provided by RefSeq, Jul 2008]
UniProt Comments for ADAMTS12
Metalloprotease that may play a role in the degradation of COMP. Cleaves also alpha-2 macroglobulin and aggregan. Has anti-tumorigenic properties.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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