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anti-AFDN antibody :: Rabbit anti-Human Afadin Polyclonal Antibody

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Catalog # MBS9600643
Unit / Price
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  0.1 mL  /  $255 +1 FREE 8GB USB
  0.2 mL  /  $295 +1 FREE 8GB USB
Product Name

Afadin (AFDN), Polyclonal Antibody

Popular Item
Full Product Name

Phospho-Afadin (Ser1799) Antibody

Product Synonym Names
AF6; AFAD_HUMAN; Afadin; ALL1-fused gene from chromosome 6 protein; Mllt4; Protein Af-6; Protein AF6
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P55196
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Specificity
Phospho-Afadin (Ser1799) antibody detects endogenous levels of Afadin.
Purity/Purification
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (lot specific)
Immunogen
A synthesized peptide derived from human Afadin around the phosphorylation site of Ser1799.
Subcellular Location
Cell Junction > Adherens Junction. Not found at cell-matrix AJs.
Predicted Cross Reactivity
Bovine, Sheep, Rabbit, Dog, Chicken, Xenopus
Similarity
Bovine (92%), Sheep (92%), Rabbit (100%), Dog (100%), Chicken (100%), Xenopus (92%)
Conjugation
Unconjugated
Epitope
Phospho Ser1799
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Supply Chain Verification
Manufactured in a lab with traceable raw materials manufactured on site. Coordinated product portfolio of antibodies, pairs, conjugates, recombinant proteins, and immunoassay materials available, please inquire.
Other Notes
Small volumes of anti-AFDN antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-AFDN antibody
Function: Belongs to an adhesion system, probably together with the E-cadherin-catenin system, which plays a role in the organization of homotypic, interneuronal and heterotypic cell-cell adherens junctions (AJs). Nectin-and actin-filament-binding protein that connects nectin to the actin cytoskeleton.
Subunit Structure: Homodimer. Interacts with F-actin, nectin and NECTIN3. Essential for the association of nectin and E-cadherin. Isoform 1/s-afadin does not interact with F-actin. Interacts with ZO-1 and occludin, but probably in an indirect manner. Interacts with RIT1 and RIT2 (By similarity). Interacts with NRXN1 and BCR.
Similarity: The PDZ/DHR domain interacts with the C-terminus of nectin and the Pro-rich C-terminal domain interacts with F-actin.
Applications Tested/Suitable for anti-AFDN antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-AFDN antibody
WB: 1:500-1:2000
IHC: 1:50-1:200

Immunohistochemistry (IHC) of anti-AFDN antibody
MBS9600643 at 1/100 staining Human thyroid cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
anti-AFDN antibody Immunohistochemistry (IHC) (IHC) image
Western Blot (WB) of anti-AFDN antibody
Western blot analysis of extracts of A431 cells treated with EGF, using Phospho-Afadin (Ser1799) Antibody.
anti-AFDN antibody Western Blot (WB) (WB) image
NCBI/Uniprot data below describe general gene information for AFDN. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
Observed: 205 kDa
Predicted: 207 kDa
NCBI Official Full Name
afadin isoform 2
NCBI Official Synonym Full Names
afadin, adherens junction formation factor
NCBI Official Symbol
NCBI Official Synonym Symbols
AF6; MLLT4; MLL-AF6; l-afadin
  [Similar Products]
NCBI Protein Information
afadin
UniProt Protein Name
Afadin
UniProt Synonym Protein Names
ALL1-fused gene from chromosome 6 protein; Protein AF-6; Afadin adherens junction formation factor
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
AF6; MLLT4; Protein AF-6  [Similar Products]
NCBI Summary for AFDN
This gene encodes a multi-domain protein involved in signaling and organization of cell junctions during embryogenesis. It has also been identified as the fusion partner of acute lymphoblastic leukemia (ALL-1) gene, involved in acute myeloid leukemias with t(6;11)(q27;q23) translocation. Alternatively spliced transcript variants encoding different isoforms have been described for this gene, however, not all have been fully characterized.[provided by RefSeq, May 2011]
UniProt Comments for AFDN
Belongs to an adhesion system, probably together with the E-cadherin-catenin system, which plays a role in the organization of homotypic, interneuronal and heterotypic cell-cell adherens junctions (AJs). Nectin- and actin-filament-binding protein that connects nectin to the actin cytoskeleton.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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