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anti-ANAPC10 antibody :: Rabbit anti-Human ANAPC10 / APC10 Polyclonal Antibody

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Catalog # MBS240619
Unit / Price
  0.05 mg  /  $530 +1 FREE 8GB USB
Product Name

ANAPC10 / APC10, Polyclonal Antibody

Also Known As

Rabbit Polyclonal to Human ANAPC10 / APC10

Product Synonym Names
Anti-ANAPC10 / APC10 Antibody (aa10-20) IHC-plus; ANAPC10; Cyclosome subunit 10; DKFZP564L0562; APC10; DOC1; Human ANAPC10; APC10
Product Synonym Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
185
OMIM
613745
3D Structure
ModBase 3D Structure for Q9UM13
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human
Specificity
aa 10-20 of Human APC10 (Anaphase-promoting complex or cyclosome).
Purity/Purification
Immunoaffinity Purified
Form/Format
0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
Concentration
1 mg/ml (lot specific)
Target Species
Human
Immunogen Description
Synthetic peptide from human ANAPC10 / APC10.
Immunogen Type
Synthetic peptide
Immunogen
ANAPC10 / APC10 antibody was raised against synthetic peptide from human ANAPC10 / APC10.
Antigen Modification
aa10-20
Preparation and Storage
+4 degree C or -20 degree C, Avoid repeated freezing and thawing.
Other Notes
Small volumes of anti-ANAPC10 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-ANAPC10 antibody
Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
Application Notes for anti-ANAPC10 antibody
ELISA, IHC-P (2.5 ug/ml), WB
Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antib...

Immunohistochemistry (IHC) of anti-ANAPC10 antibody
Anti-ANAPC10 / APC10 antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
anti-ANAPC10 antibody Immunohistochemistry (IHC) (IHC) image
Western Blot (WB) of anti-ANAPC10 antibody
Anti-APC10 Antibody - Western Blot. Affinity Purified Rabbit anti-APC10 was used at a 1:500 dilution to detect human APC10 in various cell extracts. This antibody clearly detects a ~26 kD band corresponding to human APC10 (predicted molecular weight is 21 kD). All lanes contain 20 ug of lysate or extract as follows: lane 1, HeLa nuclear extract; lane 2, HeLa whole cell lysate; lane 3, A431 whole cell lysate; lane 4, Jurkat whole cell lysate; lane 5, 293 whole cell lysate. Primary antibody was reacted with the membrane at room temperature for 1 h. After subsequent washing, a 1:5000 dilution of HRP conjugated Gt-a-Rabbit IgG was used for visualization. Exposure time was 4 min.
anti-ANAPC10 antibody Western Blot (WB) (WB) image
Western Blot (WB) of anti-ANAPC10 antibody
Anti-APC10 Antibody - Western Blot. Affinity Purified Rabbit anti-APC10 was used at a 1:500 dilution to detect human APC10 by western blot. Both HeLa whole cell lysate (lane 1) and nuclear lysate (lane 2) were probed using this antibody. Approximately 20 ug of each lysate was loaded onto a 10% SDS-PAGE gel. Primary antibody was reacted with the membrane at room temperature for 1 h. After subsequent washing, a 1:2000 dilution of HRP conjugated Gt-a-Rabbit IgG was used for visualization. Exposure time was 5 min. The expected molecular weight of human APC10 is 21 kD.
anti-ANAPC10 antibody Western Blot (WB) (WB) image
NCBI/Uniprot data below describe general gene information for ANAPC10. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
21,252 Da
NCBI Official Full Name
anaphase-promoting complex subunit 10 isoform 1
NCBI Official Synonym Full Names
anaphase promoting complex subunit 10
NCBI Official Symbol
ANAPC10  [Similar Products]
NCBI Official Synonym Symbols
DOC1; APC10
  [Similar Products]
NCBI Protein Information
anaphase-promoting complex subunit 10; cyclosome subunit 10
UniProt Protein Name
Anaphase-promoting complex subunit 10
UniProt Synonym Protein Names
Cyclosome subunit 10
UniProt Gene Name
ANAPC10  [Similar Products]
UniProt Synonym Gene Names
APC10; APC10  [Similar Products]
UniProt Entry Name
APC10_HUMAN
NCBI Summary for ANAPC10
ANAPC10 is a core subunit of the anaphase-promoting complex (APC), or cyclosome, a ubiquitin protein ligase that is essential for progression through the cell cycle. APC initiates sister chromatid separation by ubiquitinating the anaphase inhibitor securin (PTTG1; MIM 604147) and triggers exit from mitosis by ubiquitinating cyclin B (CCNB1; MIM 123836), the activating subunit of cyclin-dependent kinase-1 (CDK1; MIM 116940) (summary by Wendt et al., 2001 [PubMed 11524682]).[supplied by OMIM, Feb 2011]
UniProt Comments for ANAPC10
APC10: Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains. Belongs to the APC10 family.

Protein type: Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 4q31

Cellular Component: nucleoplasm; anaphase-promoting complex; cytosol

Biological Process: positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; mitosis; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; cell division; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; mitotic cell cycle spindle assembly checkpoint; mitotic cell cycle
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Pathways associated with anti-ANAPC10 antibody
 Products by Pathway  Pathway Diagram
 APC-Cdc20 Mediated Degradation Of Nek2A Pathway antibodies  APC-Cdc20 Mediated Degradation Of Nek2A Pathway Diagram
 APC/C Complex Pathway antibodies  APC/C Complex Pathway Diagram
 APC/C Complex Pathway antibodies  APC/C Complex Pathway Diagram
 APC/C-mediated Degradation Of Cell Cycle Proteins Pathway antibodies  APC/C-mediated Degradation Of Cell Cycle Proteins Pathway Diagram
 APC/C:Cdc20 Mediated Degradation Of Cyclin B Pathway antibodies  APC/C:Cdc20 Mediated Degradation Of Cyclin B Pathway Diagram
 APC/C:Cdc20 Mediated Degradation Of Securin Pathway antibodies  APC/C:Cdc20 Mediated Degradation Of Securin Pathway Diagram
 APC/C:Cdc20 Mediated Degradation Of Mitotic Proteins Pathway antibodies  APC/C:Cdc20 Mediated Degradation Of Mitotic Proteins Pathway Diagram
 APC/C:Cdh1 Mediated Degradation Of Cdc20 And Other APC/C:Cdh1 Targeted Proteins In Late Mitosis/early G1 Pathway antibodies  APC/C:Cdh1 Mediated Degradation Of Cdc20 And Other APC/C:Cdh1 Targeted Proteins In Late Mitosis/early G1 Pathway Diagram
 APC:Cdc20 Mediated Degradation Of Cell Cycle Proteins Prior To Satisfation Of The Cell Cycle Checkpoint Pathway antibodies  APC:Cdc20 Mediated Degradation Of Cell Cycle Proteins Prior To Satisfation Of The Cell Cycle Checkpoint Pathway Diagram
 Activation Of APC/C And APC/C:Cdc20 Mediated Degradation Of Mitotic Proteins Pathway antibodies  Activation Of APC/C And APC/C:Cdc20 Mediated Degradation Of Mitotic Proteins Pathway Diagram
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