• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

anti-Apaf-1 antibody :: Rabbit anti-Human Apaf-1 Polyclonal Antibody

Scan QR to view Datasheet
Catalog # MBS440041
Unit / Price
  0.2 mg  /  $240 +1 FREE 8GB USB
anti-Apaf-1 antibody
Product Name

Apaf-1, Polyclonal Antibody

Also Known As

Apaf-1 (A15) Antibody

Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
1248
OMIM
602233
3D Structure
ModBase 3D Structure for O14727
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human
Specificity
Human Apaf-1
Form/Format
200 ug/ml rabbit polyclonal IgG in 1 ml PBS containing 0.1 % sodium azide and 0.2% gelatin.
Concentration
0.200 mg/ml (lot specific)
Origin
Apaf-1 (A15) is provided as an affinity purified rabbit polyclonal antibody, raised against a peptide mapping to the amino terminus of human Apaf-1.
Immunogen
Synthetic peptide mapping at the amino terminal domain of human PCNA
Preparation and Storage
Store this product at 4 degree C, do not freeze. The product is stable for one year from the date of shipment.
Other Notes
Small volumes of anti-Apaf-1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-Apaf-1 antibody
Large multi-protein complexes known as the DISC (death inducing signalling complex) and the apoptosome activate the normally inactive caspases through proteolytic processing (1). The formation of these complexes leads to the activation of caspases and the execution of apoptosis (1,2). The apoptosome is a large 700 or 1400 kDa protein complex comprised of Apaf-1 (apoptotic protease activating factor-1), cytochrome c, and dATP (1). Apaf-1 and cytochrome c in the presence of dATP leads to the oligomerization of Apaf-1 and the formation of either the 700 or 1400 kDa complexes (3&4). Once formed the apoptosome activates caspase-9, which in turn activates the effector caspases (1&5). Some researchers have found the 700 kDa apoptosome to be the most effective activator of the effector caspases, when compared to the 1400 kDa apoptosome (3&4). Apaf-1 has also been shown to be a target of regulation by a variety of proteins that include p53, IAPs, heat shock proteins and Smac/Diablo (1&6).
Applications Tested/Suitable for anti-Apaf-1 antibody
Western Blot (WB)
NCBI/Uniprot data below describe general gene information for Apaf-1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
37,976 Da
NCBI Official Full Name
Apoptotic protease-activating factor 1
NCBI Official Synonym Full Names
apoptotic peptidase activating factor 1
NCBI Official Symbol
APAF1  [Similar Products]
NCBI Official Synonym Symbols
CED4; APAF-1
  [Similar Products]
NCBI Protein Information
apoptotic protease-activating factor 1
UniProt Protein Name
Apoptotic protease-activating factor 1
UniProt Gene Name
APAF1  [Similar Products]
UniProt Synonym Gene Names
KIAA0413; APAF-1  [Similar Products]
UniProt Entry Name
APAF_HUMAN
NCBI Summary for Apaf-1
This gene encodes a cytoplasmic protein that initiates apoptosis. This protein contains several copies of the WD-40 domain, a caspase recruitment domain (CARD), and an ATPase domain (NB-ARC). Upon binding cytochrome c and dATP, this protein forms an oligomeric apoptosome. The apoptosome binds and cleaves caspase 9 preproprotein, releasing its mature, activated form. Activated caspase 9 stimulates the subsequent caspase cascade that commits the cell to apoptosis. Alternative splicing results in several transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]
UniProt Comments for Apaf-1
APAF: Oligomeric Apaf-1 mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis. This activation requires ATP. Isoform 6 is less effective in inducing apoptosis. Monomer. Oligomerizes upon binding of cytochrome c and dATP. Oligomeric Apaf-1 and pro-caspase-9 bind to each other via their respective NH2-terminal CARD domains and consecutively mature caspase-9 is released from the complex. Pro-caspase-3 is recruited into the Apaf-1-pro-caspase-9 complex via interaction with pro-caspase-9. Interacts with APIP. Interacts (via CARD and NACHT domains) with NAIP/BIRC1 (via NACHT domain). By E2F and p53/TP53 in apoptotic neurons. Ubiquitous. Highest levels of expression in adult spleen and peripheral blood leukocytes, and in fetal brain, kidney and lung. Isoform 1 is expressed in heart, kidney and liver. 6 isoforms of the human protein are produced by alternative splicing.

Protein type: Apoptosis

Chromosomal Location of Human Ortholog: 12q23

Cellular Component: cytosol; nucleus; apoptosome

Molecular Function: identical protein binding; protein binding; nucleotide binding; caspase activator activity; ADP binding; ATP binding

Biological Process: caspase activation; regulation of apoptosis; nervous system development; neuron apoptosis; apoptosis; positive regulation of apoptosis; neural tube closure; forebrain development; caspase activation via cytochrome c
Product References and Citations for anti-Apaf-1 antibody
1. Cain K., Bratton S.B., Cohen G.M. 2002. The Apaf-1 apoptosome: a large caspase-activating complex. Biochimie 84 (2-3):203-214.2. Bratton S.B., Lewis J., Butterworth M., Duckett C.S., Cohen G.M. 2002. XIAP inhibition of caspase-3 preserves its association with the Apaf-1 apoptosome and prevents CD95- and Bax-induced apoptosis. Cell Death Differ 9(9):881-892.3. Bratton S.B., Walker G., Roberts D.L., Cain K., Cohen G.M. 2001. Caspase-3 cleaves Apaf-1 into an ~30kDa fragment that associates with an inappropriately oligomerized and biologically inactive ~1.4 Mda apoptosome complex. Cell Death Differ 8(4):425-433.4. Cain K., Bratton S.B., Langlais C., Walker G., Brown D.G., Sun X.M., Cohen G.M. 2000. Apaf-1 oligomerizes into biologically active approximately 700-kDa and inactive approximately 1.4-Mda apoptosome complex. J Biol Chem 275(9):6067-6070.5. Zou H., Li Y., Liu X., Wang X. 1999. An Apaf-1.cytochrome c multimeric complex is a functional apoptosome that activates procaspase-9. J Biol Chem 274(17):11549-11556.6. Robles A.I., Bemmels N.A., Foraker A.B., Harris C.C. 2001. Apaf-1 is a transcriptional target of p53 in DNA damage-induced apoptosis. Cancer Res 61(18):6660-6664.

Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000