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anti-ARFGAP3 antibody :: Rabbit anti-Human ARFGAP3 Polyclonal Antibody

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Catalog # MBS1493385
Unit / Price
  0.05 mL  /  $120 +1 FREE 8GB USB
  0.1 mL  /  $160 +1 FREE 8GB USB
Product Name

ARFGAP3, Polyclonal Antibody

Popular Item
Also Known As

ARFGAP3 Antibody

Product Synonym Names
ARF GAP 3; ARFGAP3; ARFGAP1
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
472
OMIM
612439
3D Structure
ModBase 3D Structure for Q9NP61
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Purity/Purification
Antigen Affinity Purified
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Conjugate
Non-conjugated
Immunogen
Recombinant human ADP-ribosylation factor GTPase-activating protein 3 protein (217-516aa)
Santa Cruz Alternative
Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-46784 / sc-46779 / sc-292277
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Other Notes
Small volumes of anti-ARFGAP3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-ARFGAP3 antibody
GTPase-activating protein (GAP) for ADP ribosylation factor 1 (ARF1). Hydrolysis of ARF1-bound GTP may lead to dissociation of coatomer from Golgi-derived membranes to allow fusion with target membranes.
Applications Tested/Suitable for anti-ARFGAP3 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP)
Application Notes for anti-ARFGAP3 antibody
Recommended dilution: WB:1:500-2000
IHC:1:20-1:200

Western Blot (WB) of anti-ARFGAP3 antibody
Western blot
All lanes: ARFGAP3 antibody at 4ug/ml
Lane 1:HepG2 whole cell lysate
Lane 2:Jurkat whole cell lysate
Lane 3:A549 whole cell lysate
Secondary
Goat polyclonal to rabbit at 1/10000 dilution
Predicted band size: 57,52 kDa
Observed band size: 57 kDa

anti-ARFGAP3 antibody Western Blot (WB) (WB) image
Immunohistochemistry (IHC) of anti-ARFGAP3 antibody
Immunohistochemistry of paraffin-embedded human breast cancer using MBS1493385 at dilution 1:100
anti-ARFGAP3 antibody Immunohistochemistry (IHC) (IHC) image
Immunohistochemistry (IHC) of anti-ARFGAP3 antibody
Immunohistochemistry of paraffin-embedded human liver cancer using MBS1493385 at dilution 1:100
anti-ARFGAP3 antibody Immunohistochemistry (IHC) (IHC) image
Immunoprecipitation (IP) of anti-ARFGAP3 antibody
Immunoprecipitating ARFGAP3 in HepG2 whole cell lysate
Lane 1: Rabbit monoclonal IgG(1ug)instead of MBS1493385 in HepG2 whole cell lysate. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the Secondary antibody (1/50000)
Lane 2:MBS1493385(4ug)+ HepG2 whole cell lysate(500ug)
Lane 3: HepG2 whole cell lysate (20ug)

anti-ARFGAP3 antibody Immunoprecipitation (IP) (IP) image
NCBI/Uniprot data below describe general gene information for ARFGAP3. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
51,990 Da
NCBI Official Full Name
ADP-ribosylation factor GTPase-activating protein 3 isoform 2
NCBI Official Synonym Full Names
ADP ribosylation factor GTPase activating protein 3
NCBI Official Symbol
ARFGAP3  [Similar Products]
NCBI Official Synonym Symbols
ARFGAP1
  [Similar Products]
NCBI Protein Information
ADP-ribosylation factor GTPase-activating protein 3
UniProt Protein Name
ADP-ribosylation factor GTPase-activating protein 3
UniProt Gene Name
ARFGAP3  [Similar Products]
UniProt Synonym Gene Names
ARFGAP1; ARF GAP 3  [Similar Products]
UniProt Entry Name
ARFG3_HUMAN
NCBI Summary for ARFGAP3
The protein encoded by this gene is a GTPase-activating protein (GAP) that associates with the Golgi apparatus and regulates the early secretory pathway of proteins. The encoded protein promotes hydrolysis of ADP-ribosylation factor 1 (ARF1)-bound GTP, which is required for the dissociation of coat proteins from Golgi-derived membranes and vesicles. Dissociation of the coat proteins is a prerequisite for the fusion of these vesicles with target compartments. The activity of this protein is sensitive to phospholipids. Multiple transcript variants encoding different isoforms have been found for this gene. This gene was originally known as ARFGAP1, but that is now the name of a related but different gene. [provided by RefSeq, Nov 2008]
UniProt Comments for ARFGAP3
GTPase-activating protein (GAP) for ADP ribosylation factor 1 (ARF1). Hydrolysis of ARF1-bound GTP may lead to dissociation of coatomer from Golgi-derived membranes to allow fusion with target membranes.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Products associated withanti-ARFGAP3 antibody
 Reference Product  PubMed Publications
 ARF1 antibody  >10 publications with ARFGAP3 and ARF1
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