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anti-ASNS antibody :: Rabbit anti-Human Asparagine Synthetase Polyclonal Antibody

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Catalog # MBS2027582 (SPECIAL PROMOTION: Get FREE $5 Starbucks gift card (Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Unit / Price
  0.01 mg  /  $110 +1 FREE 8GB USB
  0.02 mg  /  $130 +1 FREE 8GB USB
  0.05 mg  /  $175 +1 FREE 8GB USB
  0.1 mg  /  $225 +1 FREE 8GB USB
  0.2 mg  /  $340 +1 FREE 8GB USB
  1 mg  /  $800 +1 FREE 8GB USB
Product Name

Asparagine Synthetase (ASNS), Polyclonal Antibody

Popular Item
Also Known As

Polyclonal Antibody to Asparagine Synthetase (ASNS)

Matching Pairs
Matching Pairs
Unconjugated Antibody: Asparagine Synthetase (MBS2027582)
APC-CY7 Conjugated Antibody: Asparagine Synthetase (ASNS) (MBS2072787)
Matching Pairs
Matching Pairs
Matching Pairs
Matching Pairs
Matching Pairs
Matching Pairs
Unconjugated Antibody: Asparagine Synthetase (MBS2027582)
APC-CY7 Conjugated Secondary Antibody: Immunoglobulin G (MBS2090675)
Matching Pairs
Unconjugated Antibody: Asparagine Synthetase (MBS2027582)
Unconjugated Secondary Antibody: Immunoglobulin G (MBS2090678)
Matching Pairs
Unconjugated Antibody: Asparagine Synthetase (MBS2027582)
Biotin Conjugated Antibody: Asparagine Synthetase (ASNS) (MBS2095488)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
OMIM
M27396 mRNA
3D Structure
ModBase 3D Structure for P08243
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Specificity
The antibody is a rabbit polyclonal antibody raised against ASNS. It has beenselected for its ability to recognize ASNS in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Liquid
Concentration
200ug/ml (lot specific)
Fragment
ASNS (His213~Tyr536)
Organism Species
Homo sapiens (Human)
Conjugate
No Conjugate
Immunogen
Recombinant ASNS (His213~Tyr536) expressed in E Coli.
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2072789
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Other Notes
Small volumes of anti-ASNS antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Product Categories/Family for anti-ASNS antibody
Applications Tested/Suitable for anti-ASNS antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA)
Application Notes for anti-ASNS antibody
Western blotting: 1-5ug/mL
Immunocytochemistry in formalin fixed cells: 5-20ug/mL
Immunohistochemistry in formalin fixed frozen section: 5-20ug/mL
Immunohistochemistry in paraffin section: 5-20ug/mL
Enzyme-linked Immunosorbent Assay: 0.05-2ug/mL
Optimal working dilutions must be determined by end user.

Western Blot (WB) of anti-ASNS antibody
Western Blot: Sample: Lane1: Human Jurkat Cells; Lane2: Human K-562 Cells.
anti-ASNS antibody Western Blot (WB) (WB) image
Western Blot (WB) of anti-ASNS antibody
Western Blot: Sample: Recombinant ASNS, Human.
anti-ASNS antibody Western Blot (WB) (WB) image
Immunohistochemistry (IHC) of anti-ASNS antibody
DAB staining on IHC-P. Samples: Human Tissue)
anti-ASNS antibody Immunohistochemistry (IHC) (IHC) image
Knockout Validation of anti-ASNS antibody
Knockout Validation: Lane 1: Wild-type K562 cell lysate; Lane 2: ASNS knockout K562 cell lysate; Predicted MW: 64,62,54kd Observed MW: 62kd Primary Ab: 3ug/ml Rabbit Anti-Human ASNS Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (#MBS2086047)
anti-ASNS antibody Knockout Validation image
NCBI/Uniprot data below describe general gene information for ASNS. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
54,818 Da
NCBI Official Full Name
asparagine synthetase
NCBI Official Synonym Full Names
asparagine synthetase (glutamine-hydrolyzing)
NCBI Official Symbol
NCBI Official Synonym Symbols
TS11; ASNSD
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NCBI Protein Information
asparagine synthetase [glutamine-hydrolyzing]
UniProt Protein Name
Asparagine synthetase [glutamine-hydrolyzing]
UniProt Synonym Protein Names
Cell cycle control protein TS11; Glutamine-dependent asparagine synthetase
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
NCBI Summary for ASNS
The protein encoded by this gene is involved in the synthesis of asparagine. This gene complements a mutation in the temperature-sensitive hamster mutant ts11, which blocks progression through the G1 phase of the cell cycle at nonpermissive temperature. Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, May 2010]
UniProt Comments for ASNS
ASNS: asparagine synthetase, an ubiquitous enzyme in mammalian organs, is involved in the first step of the synthesis of L-asparagine from L-glutamine and L-aspartate. Highly regulated in response to cell stress. Induced by dietary protein deficiency, imbalanced amino acid intake via the amino acid response, or ER stress via the EIF2AK3-eIF2-ATF4 branch of the unfolded protein response (UPR). Expression induced by nutrient deprivation or hypoxia in solid tumors. Essential for brain development but apparently not for that of other organs. Deficiency impairs brain development resulting in microcephaly and progressive cerebral atrophy, as well as deficiencies in learning and memory. The mechanism through which ASNS induces neuronal damage may be via damage induced by excessive excitability induced by elevated levels of aspartate/glutamate, via asparagine deficiency, or both. Three isoforms of the human protein are produced by alternative splicing.

Protein type: Amino Acid Metabolism - alanine, aspartate and glutamate; Cell cycle regulation; Cell development/differentiation; EC 6.3.5.4; Energy Metabolism - nitrogen; Ligase

Chromosomal Location of Human Ortholog: 7q21.3

Cellular Component: cytosol

Molecular Function: asparagine synthase (glutamine-hydrolyzing) activity; ATP binding; cofactor binding; protein binding; protein homodimerization activity

Biological Process: amino acid biosynthetic process; asparagine biosynthetic process; cellular response to glucose starvation; cellular response to hormone stimulus; glutamine metabolic process; liver development; negative regulation of apoptosis; positive regulation of mitotic cell cycle; response to amino acid; response to follicle-stimulating hormone stimulus; response to light stimulus; response to mechanical stimulus; response to methotrexate; response to toxin

Disease: Asparagine Synthetase Deficiency
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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