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anti-GOT1 antibody :: Rabbit anti-Human Aspartate aminotransferase Polyclonal Antibody

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Catalog # MBS1488479
Unit / Price
Scan QR to view Datasheet
  0.05 mg  /  $160 +1 FREE 8GB USB
  0.1 mg  /  $235 +1 FREE 8GB USB
Product Name

Aspartate aminotransferase (GOT1), Polyclonal Antibody

Popular Item
Full Product Name

Rabbit anti-human Aspartate aminotransferase, cytoplasmic polyclonal Antibody

Product Synonym Names
Glutamate oxaloacetate transaminase 1; Transaminase; GOT1
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
OMIM
138180
3D Structure
ModBase 3D Structure for P17174
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Purity/Purification
Caprylic Acid Ammonium Sulfate Precipitation Purified
Storage Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Conjugate
Non-conjugated
Immunogen
Recombinant human Aspartate aminotransferase, cytoplasmic protein
Santa Cruz Alternative
Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-46283 / sc-46281
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Other Notes
Small volumes of anti-GOT1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-GOT1 antibody
Plays a key role in amino acid metabolism.
Applications Tested/Suitable for anti-GOT1 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)

Western Blot (WB) of anti-GOT1 antibody
All lanes: Aspartate aminotransferase, cytoplasmic antibody at 2ug/ml+mouse brain tissue
Secondary
Goat polyclonal to Rabbit IgG at 1/10000 dilution
Predicted band size: 47,45 kDa
Observed band size: 46 kDa

anti-GOT1 antibody Western Blot (WB) (WB) image
Western Blot (WB) of anti-GOT1 antibody
Western Blot
Positive WB detected in: hepG2 cell,mouse liver,mouse skeletal muscle
All lanes: GOT1 antibody at 4.5ug/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 47,45 kDa
Observed band size: 46 kDa

anti-GOT1 antibody Western Blot (WB) (WB) image
Immunohistochemistry (IHC) of anti-GOT1 antibody
Immunohistochemistry of paraffin-embedded mouse brain using MBS1488479 at dilution of 1:50
anti-GOT1 antibody Immunohistochemistry (IHC) (IHC) image
Immunofluorescence (IF) of anti-GOT1 antibody
Immunofluorescent analysis of HepG2 cells using MBS1488479 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
anti-GOT1 antibody Immunofluorescence (IF) image
NCBI/Uniprot data below describe general gene information for GOT1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
44,147 Da[Similar Products]
NCBI Official Full Name
aspartate aminotransferase, cytoplasmic
NCBI Official Synonym Full Names
glutamic-oxaloacetic transaminase 1, soluble
NCBI Official Symbol
NCBI Official Synonym Symbols
AST1; cCAT; GIG18; cAspAT; ASTQTL1
  [Similar Products]
NCBI Protein Information
aspartate aminotransferase, cytoplasmic
UniProt Protein Name
Aspartate aminotransferase, cytoplasmic
UniProt Synonym Protein Names
Cysteine aminotransferase, cytoplasmic; Cysteine transaminase, cytoplasmic; cCAT; Glutamate oxaloacetate transaminase 1; Transaminase A
UniProt Gene Name
UniProt Synonym Gene Names
cAspAT; cCAT  [Similar Products]
UniProt Entry Name
AATC_HUMAN
NCBI Summary for GOT1
Glutamic-oxaloacetic transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology. [provided by RefSeq, Jul 2008]
UniProt Comments for GOT1
GOT1: Plays a key role in amino acid metabolism. Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.

Protein type: Transferase; Amino Acid Metabolism - tyrosine; Amino Acid Metabolism - cysteine and methionine; Amino Acid Metabolism - arginine and proline; Amino Acid Metabolism - phenylalanine; Amino Acid Metabolism - phenylalanine, tyrosine and tryptophan biosynthesis; EC 2.6.1.3; EC 2.6.1.1; Amino Acid Metabolism - alanine, aspartate and glutamate

Chromosomal Location of Human Ortholog: 10q24.1-q25.1

Cellular Component: cytoplasm; cytosol; lysosome; mitochondrion; nerve terminal; nucleus

Molecular Function: aspartate transaminase activity; carboxylic acid binding; cysteine transaminase activity; identical protein binding; phosphatidylserine decarboxylase activity; pyridoxal phosphate binding

Biological Process: 2-oxoglutarate metabolic process; amino acid biosynthetic process; aspartate biosynthetic process; aspartate catabolic process; aspartate metabolic process; carbohydrate metabolic process; cellular response to insulin stimulus; fatty acid homeostasis; gluconeogenesis; glucose metabolic process; glutamate catabolic process to 2-oxoglutarate; glutamate catabolic process to aspartate; glutamate metabolic process; glycerol biosynthetic process; methionine salvage; Notch signaling pathway; oxaloacetate metabolic process; polyamine metabolic process; response to glucocorticoid stimulus; sulfur amino acid metabolic process

Disease: Aspartate Aminotransferase, Serum Level Of, Quantitative Trait Locus 1
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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