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anti-AGA antibody :: Rabbit anti-Human Aspartylglucosaminidase Polyclonal Antibody

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Catalog # MBS2032278 (SPECIAL PROMOTION: Get FREE $5 Starbucks gift card (Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Unit / Price
Scan QR to view Datasheet
  0.01 mg  /  $110 +1 FREE 8GB USB
  0.02 mg  /  $130 +1 FREE 8GB USB
  0.05 mg  /  $175 +1 FREE 8GB USB
  0.1 mg  /  $225 +1 FREE 8GB USB
  0.2 mg  /  $340 +1 FREE 8GB USB
  1 mg  /  $800 +1 FREE 8GB USB
Product Name

Aspartylglucosaminidase (AGA), Polyclonal Antibody

Full Product Name

Aspartylglucosaminidase (AGA) Polyclonal Antibody

Product Synonym Names
AGU; ASRG; GA; N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase; Glycosylasparaginase; N4-(N-acetyl-beta-glucosaminyl)-L-asparagine amidase
Product Gene Name
Matching Pairs
Matching Pairs
Unconjugated Antibody: Aspartylglucosaminidase (MBS2032278)
APC-CY7 Conjugated Antibody: Aspartylglucosaminidase (AGA) (MBS2064883)
Matching Pairs
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
OMIM
X55762 mRNA
3D Structure
ModBase 3D Structure for P20933
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Specificity
The antibody is a rabbit polyclonal antibody raised against AGA. It has been selected for its ability to recognize AGA in immunohistochemical staining and western blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4 containing 0.02% NaN3, 50% glycerol.
Concentration
200ug/ml (lot specific)
Immunoglobulin Type
IgG
Conjugate
No Conjugate
Organism Species
Human
Fragment
AGA (Pro28~Ala245)
Quality Control
Content: The quality control contains recombinant AGA (Pro28~Ala245) disposed in loading buffer.
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
5uL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol,BPB 0.01%, NaN3 0.02%.
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2064885
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
ISO Certification
Manufactured in an ISO 9001:2008 and ISO 13485:2003 Certified Laboratory.
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.
Other Notes
Small volumes of anti-AGA antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-AGA antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA)
Application Notes for anti-AGA antibody
Western Blot: 1:50-400
Immunohistochemistry in formalin fixed frozen section: 1:50-500
Enzyme-linked Immunosorbent Assay: 1:100-200

Western Blot (WB) of anti-AGA antibody
Western Blot: Sample: Human 293T Cells.
anti-AGA antibody Western Blot (WB) (WB) image
Western Blot (WB) of anti-AGA antibody
Western Blot: Sample: Recombinant AGA, Human.
anti-AGA antibody Western Blot (WB) (WB) image
Immunohistochemistry (IHC) of anti-AGA antibody
DAB staining on IHC-P; Samples: Human Prostate Gland Tissue.
anti-AGA antibody Immunohistochemistry (IHC) (IHC) image
Immunohistochemistry (IHC) of anti-AGA antibody
DAB staining on IHC-P; Samples: Human Brain Tissue
anti-AGA antibody Immunohistochemistry (IHC) (IHC) image
Immunohistochemistry (IHC) of anti-AGA antibody
DAB staining on IHC-P; Samples: Human Liver Tissue
anti-AGA antibody Immunohistochemistry (IHC) (IHC) image
NCBI/Uniprot data below describe general gene information for AGA. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
37,208 Da
NCBI Official Full Name
N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase isoform 1 preproprotein
NCBI Official Synonym Full Names
aspartylglucosaminidase
NCBI Official Symbol
NCBI Official Synonym Symbols
GA; AGU; ASRG
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NCBI Protein Information
N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase
UniProt Protein Name
N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase
UniProt Synonym Protein Names
Aspartylglucosaminidase; Glycosylasparaginase; N4-(N-acetyl-beta-glucosaminyl)-L-asparagine amidase
UniProt Gene Name
NCBI Summary for AGA
This gene encodes a member of the N-terminal nucleophile (Ntn) hydrolase family of proteins. The encoded preproprotein is proteolytically processed to generate alpha and beta chains that comprise the mature enzyme. This enzyme is involved in the catabolism of N-linked oligosaccharides of glycoproteins. It cleaves asparagine from N-acetylglucosamines as one of the final steps in the lysosomal breakdown of glycoproteins. Mutations in this gene are associated with the lysosomal storage disease aspartylglycosaminuria that results in progressive neurodegeneration. Alternative splicing results in multiple transcript variants, at least one of which encodes an isoform that is subject to proteolytic processing. [provided by RefSeq, Nov 2015]
UniProt Comments for AGA
AGA: a catabolic enzyme, aspartylglucosaminidase is involved in the catabolism of N-linked oligosaccharides of glycoproteins. It cleaves asparagine from N-acetylglucosamines as one of the final steps in the lysosomal breakdown of glycoproteins. The lysosomal storage disease aspartylglycosaminuria is caused by a deficiency in the AGA enzyme.

Protein type: EC 3.5.1.26; Glycan Metabolism - other glycan degradation; Hydrolase

Chromosomal Location of Human Ortholog: 4q34.3

Cellular Component: endoplasmic reticulum; extracellular exosome; extracellular region; extracellular space; lysosome

Molecular Function: N4-(beta-N-acetylglucosaminyl)-L-asparaginase activity; peptidase activity; protein self-association

Biological Process: neutrophil degranulation; protein deglycosylation; protein maturation; proteolysis

Disease: Aspartylglucosaminuria
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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