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anti-ATF3 antibody :: Rabbit anti-Human ATF3 Polyclonal Antibody

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Catalog # MBS240617
Unit / Price
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  0.05 mg  /  $530 +1 FREE 8GB USB
Product Name

ATF3, Polyclonal Antibody

Full Product Name

Rabbit Polyclonal to Human ATF3

Product Synonym Names
Anti-ATF3 Antibody (aa113-130) IHC-plus; ATF3; Human ATF3
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
OMIM
603148
3D Structure
ModBase 3D Structure for P18847
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human
Specificity
This antibody was produced from a aa 113-130 of human ATF3.
Purity/Purification
Affinity Purified
Form/Format
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01% (w/v) Sodium Azide
Concentration
1 mg/ml (lot specific)
Target Species
Human
Immunogen Description
Synthetic peptide from human ATF3.
Immunogen Type
Synthetic peptide
Immunogen
ATF3 antibody was raised against synthetic peptide from human ATF3.
Antigen Modification
aa113-130
Preparation and Storage
+4 degree C or -20 degree C, Avoid repeated freezing and thawing.
Other Notes
Small volumes of anti-ATF3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-ATF3 antibody
Activating transcription factor 3 (ATF3)is a member of the mammalian activation transcription factor/cAMP responsive element-binding (CREB) protein family of transcription factors. It encodes a protein with a calculated molecular mass of 22 kD. ATF3 represses rather than activates transcription from promoters with ATF binding elements.
Product Categories/Family for anti-ATF3 antibody
Applications Tested/Suitable for anti-ATF3 antibody
Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
Application Notes for anti-ATF3 antibody
ELISA, IHC-P (5 ug/ml), WB (1:200)
Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antib...

Immunohistochemistry (IHC) of anti-ATF3 antibody
Anti-ATF-3 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
anti-ATF3 antibody Immunohistochemistry (IHC) (IHC) image
Western Blot (WB) of anti-ATF3 antibody
Anti-ATF3 Antibody - Western Blot. Western blot of mammalian whole cell extract transfected with HA epitope tagged human ATF3. Affinity purified anti-ATF3 detects a band ~31 kD corresponding to recombinant human ATF3. Immunostaining using anti-HA epitope tag antibody confirms the composition of the recombinant band (not shown). The protein was transferred to nitrocellulose in 30 minutes using standard methods. After blocking with 5% goat serum and 0.5% non-fat milk in PBS, the membrane was probed with the primary antibody diluted 1:200 in 0.2X blocking buffer in PBS overnight at 4C. Reaction was followed by washes and reaction with a 1:5000 dilution of IRDye800 conjugated Gt-a-Rabbit IgG [H&L] (code for 30 min at room temperature. LICORs Odyssey Infrared Imaging System was used to scan and process the image. Other detection systems will yield similar results.
anti-ATF3 antibody Western Blot (WB) (WB) image
Western Blot (WB) of anti-ATF3 antibody
Anti-ATF3 Antibody - Western Blot. Western blot of E Coli whole cell extract transfected with GST epitope tagged human ATF3. Affinity purified anti-ATF3 detects a band ~48 kD corresponding to recombinant human ATF3. Immunostaining using anti-GST epitope tag antibody confirms the composition of the recombinant band (not shown). The protein was transferred to nitrocellulose using standard methods. After blocking with 5% goat serum and 0.5% non fat milk in PBS, the membrane was probed with the primary antibody diluted 1:200 in 0.2X blocking buffer in PBS overnight at 4°C. Reaction was followed by washes and reaction with a 1:5000 dilution of IRDye800 conjugated Gt-a-Rabbit IgG [H&L] (code for 30 min at room temperature. LICORs Odyssey Infrared Imaging System was used to scan and process the image. Other detection systems will yield similar results.
anti-ATF3 antibody Western Blot (WB) (WB) image
NCBI/Uniprot data below describe general gene information for ATF3. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
14,314 Da
NCBI Official Synonym Full Names
activating transcription factor 3
NCBI Official Symbol
NCBI Protein Information
cyclic AMP-dependent transcription factor ATF-3
UniProt Protein Name
Cyclic AMP-dependent transcription factor ATF-3
UniProt Synonym Protein Names
Activating transcription factor 3
UniProt Gene Name
UniProt Synonym Gene Names
cAMP-dependent transcription factor ATF-3  [Similar Products]
UniProt Entry Name
ATF3_HUMAN
NCBI Summary for ATF3
This gene encodes a member of the mammalian activation transcription factor/cAMP responsive element-binding (CREB) protein family of transcription factors. This gene is induced by a variety of signals, including many of those encountered by cancer cells, and is involved in the complex process of cellular stress response. Multiple transcript variants encoding different isoforms have been found for this gene. It is possible that alternative splicing of this gene may be physiologically important in the regulation of target genes. [provided by RefSeq, Apr 2011]
UniProt Comments for ATF3
ATF-3: This protein binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Represses transcription from promoters with ATF sites. It may repress transcription by stabilizing the binding of inhibitory cofactors at the promoter. Isoform 2 activates transcription presumably by sequestering inhibitory cofactors away from the promoters. Belongs to the bZIP family. ATF subfamily. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription factor

Chromosomal Location of Human Ortholog: 1q32.3

Cellular Component: nucleoplasm; nucleolus; nucleus

Molecular Function: identical protein binding; protein binding; protein homodimerization activity; protein heterodimerization activity; transcription corepressor activity; transcription factor activity

Biological Process: unfolded protein response, activation of signaling protein activity; cellular protein metabolic process; transcription, DNA-dependent; unfolded protein response; positive regulation of cell proliferation; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription from RNA polymerase II promoter; gluconeogenesis
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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