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anti-BLNK antibody :: Rabbit anti-Human B Cell Linker Protein Polyclonal Antibody

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Catalog # MBS627881
Unit / Price
  0.1 mL  /  $595 +1 FREE 8GB USB
anti-BLNK antibody
Product Name

B Cell Linker Protein (BLNK), Polyclonal Antibody

Full Product Name

B Cell Linker Protein, phosphorylated (Tyr96) (BLNK, BASH, BLNK-s, Ly57, MGC111051, SLP-65, SLP65)

Product Synonym Names
Anti -B Cell Linker Protein, phosphorylated (Tyr96) (BLNK, BASH, BLNK-s, Ly57, MGC111051, SLP-65, SLP65)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Chromosome Location
Chromosome: 10; NC_000010.10 (97951455..98031333, complement). Location: 10q23.2-q23.33
3D Structure
ModBase 3D Structure for Q2MD52
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Specificity
Detects endogenous levels of Tyr96 phosphorylated BLNK.
Purity/Purification
Affinity Purified
Purified by protein A and peptide affinity chromatography.
Form/Format
Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, BSA, 50% glycerol.
Immunogen
Synthetic phosphopeptide (KLH coupled) corresponding to residues around Tyr96 of human BLNK.
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Other Notes
Small volumes of anti-BLNK antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-BLNK antibody
BLNK (B cell linker protein) is an adaptor molecule that plays key roles from B cell activation through B cell antigen receptor (BCR) engagent. BLNK acts on the inferface between BCR-associated Syk and the downstream signaling cascade (1,2). BLNK has multiple SH2 binding motifs (YXXP) at its N-terminus and an SH2 domain at its C-terminus. After BCR ligation, BLNK is phosphorylated by Syk on its multiple YXXP motifs including Tyr72, Tyr84, Tyr96 and Tyr178 (1). The phosphorylation on these motifs provides docking sites for signaling molecules such as Btk, PLCgamma and Vav. They bind to BLNK through their SH2 domains and cooperatively activate downstream signaling (3,4). BLNK can also interact with phosphorylated targets such as HPK1 through its SH2 domain to activate the pathway (5).
Product Categories/Family for anti-BLNK antibody
Applications Tested/Suitable for anti-BLNK antibody
Western Blot (WB), Immunoprecipitation (IP)
Application Notes for anti-BLNK antibody
Suitable for use in Western Blotting, Immunoprecipitation.
Dilution: Western Blot: 1:1000
Immunoprecipitation: 1:100
NCBI/Uniprot data below describe general gene information for BLNK. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Related Accession #
Molecular Weight
48,201 Da[Similar Products]
NCBI Official Full Name
B-cell linker protein isoform 2
NCBI Official Synonym Full Names
B-cell linker
NCBI Official Symbol
NCBI Official Synonym Symbols
AGM4; BASH; LY57; SLP65; BLNK-S; SLP-65; MGC111051
  [Similar Products]
NCBI Protein Information
B-cell linker protein; OTTHUMP00000020167; OTTHUMP00000020168; B cell linker protein; cytoplasmic adapter protein; B-cell adapter containing a SH2 domain protein; B-cell adapter containing a Src homology 2 domain protein; Src homology 2 domain-containing leukocyte protein of 65 kDa
UniProt Protein Name
B-cell linker protein
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
UniProt Entry Name
Q2MD52_HUMAN
NCBI Summary for BLNK
This gene encodes a cytoplasmic linker or adaptor protein that plays a critical role in B cell development. This protein bridges B cell receptor-associated kinase activation with downstream signaling pathways, thereby affecting various biological functions. The phosphorylation of five tyrosine residues is necessary for this protein to nucleate distinct signaling effectors following B cell receptor activation. Mutations in this gene cause hypoglobulinemia and absent B cells, a disease in which the pro- to pre-B-cell transition is developmentally blocked. Deficiency in this protein has also been shown in some cases of pre-B acute lymphoblastic leukemia. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]
UniProt Comments for BLNK
BLNK iso3: an adaptor protein that bridges the B-cell receptor-associated kinases (BCR) with a multitude of signaling pathways, regulating biologic outcomes of B-cell function and development. Plays an important role in BCR-mediated PLCG1 activation and Ca(2 ) mobilization. Does not seem to be not required for pre-BCR-mediated activation of MAP kinase and phosphatidyl-inositol 3 (PI3) kinase signaling. Plays a critical role in orchestrating the pro-B cell to pre-B cell transition. Following BCR activation, phosphorylated on tyrosine residues by SYK and LYN. When phosphorylated, serves as a scaffold to assemble downstream targets of antigen activation, including PLCG1, VAV1, GRB2 and NCK1. Its phosphorylation is required for both Ca(2 ) and MAPK signaling pathways. Defects in BLNK are the cause of hypoglobulinemia and absent B-cells.It has tumor supressor activity that is lost in many cases of childhood pre-B acute lymphoblastic leukemia (ALL). Two alternatively spliced isoforms have been described; these are differentially involved in activation and apoptosis of B lymphocytes.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 10q23.2-q23.33

Cellular Component: plasma membrane; intracellular; cytosol

Molecular Function: protein binding; SH3/SH2 adaptor activity; transmembrane receptor protein tyrosine kinase adaptor protein activity

Biological Process: positive regulation of signal transduction; B cell differentiation; inflammatory response; humoral immune response; transmembrane receptor protein tyrosine kinase signaling pathway
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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