• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

anti-BTRC antibody :: Rabbit anti-Human BTRC Polyclonal Antibody

Scan QR to view Datasheet
Catalog # MBS7051680
Unit / Price
Scan QR to view Datasheet
  0.05 mg  /  $160 +1 FREE 8GB USB
  0.1 mg  /  $235 +1 FREE 8GB USB
Product Name

BTRC, Polyclonal Antibody

Popular Item
Full Product Name

BTRC Antibody

Product Synonym Names
F-box/WD repeat-containing protein 1A; E3RSIkappaB; Epididymis tissue protein Li 2a; F-box and WD repeats protein beta-TrCP; pIkappaBalpha-E3 receptor subuni; BTRC; BTRCP; FBW1A; FBXW1A
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
OMIM
AF101784 mRNA
3D Structure
ModBase 3D Structure for Q9Y297
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Purity/Purification
>95%, Protein G purified
Form/Format
Liquid
Immunogen
Recombinant Human F-box/WD repeat-containing protein 1A protein (397-448AA)
Preservative
0.03% Proclin 300
Constituents
50% Glycerol, 0.01M PBS, PH 7.4
Conjugation
Non-conjugated
Santa Cruz Alternative
Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-15354 / sc-33213 / sc-8862 / sc-8863
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Other Notes
Small volumes of anti-BTRC antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-BTRC antibody
Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Recognizes and binds to phosphorylated target proteins. SCF(BTRC) mediates the ubiquitination of CTNNB1 and participates in Wnt signaling. SCF(BTRC) mediates the ubiquitination of NFKBIA, NFKBIB and NFKBIE; the degradation frees the associated NFKB1 to translocate into the nucleus and to activate transcription. Ubiquitination of NFKBIA occurs at 'Lys-21' and 'Lys-22'. SCF(BTRC) mediates the ubiquitination of CEP68; this is required for centriole separation during mitosis (PubMed: 25704143, PubMed: 25503564). SCF(BTRC) mediates the ubiquitination of phosphorylated NFKB1/nuclear factor NF-kappa-B p105 subunit, ATF4, CDC25A, DLG1, FBXO5, PER1, SMAD3, SMAD4, SNAI1 and probably NFKB2. Has an essential role in the control of the clock-dependent transcription via degradation of phosphorylated PER1 and PER2. May be involved in ubiquitination and subsequent proteasomal degradation through a DBB1-CUL4 E3 ubiquitin-protein ligase. Required for activation of NFKB-mediated transcription by IL1B, MAP3K14, MAP3K1, IKBKB and TNF. Required for proteolytic processing of GLI3.
Applications Tested/Suitable for anti-BTRC antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes for anti-BTRC antibody
WB: 1: 2000-1: 10000
IHC: 1: 20-1: 200
IF: 1: 50-1: 200

Western Blot (WB) of anti-BTRC antibody
Western Blot
Positive WB detected in:K562 whole cell lysate
All lanes: BTRC antibody at 2.8ug/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 69,66 kDa
Observed band size: 69 kDa

anti-BTRC antibody Western Blot (WB) (WB) image
Immunohistochemistry (IHC) of anti-BTRC antibody
Immunohistochemistry of paraffin-embedded human pancreatic tissue using MBS7051680 at dilution of 1:100
anti-BTRC antibody Immunohistochemistry (IHC) (IHC) image
Immunohistochemistry (IHC) of anti-BTRC antibody
Immunofluorescent analysis of HepG2 cells using MBS7051680 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
anti-BTRC antibody Immunohistochemistry (IHC) (IHC) image
NCBI/Uniprot data below describe general gene information for BTRC. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
65,049 Da
NCBI Official Full Name
F-box/WD repeat-containing protein 1A isoform 2
NCBI Official Synonym Full Names
beta-transducin repeat containing E3 ubiquitin protein ligase
NCBI Official Symbol
NCBI Official Synonym Symbols
FWD1; FBW1A; FBXW1; bTrCP; FBXW1A; bTrCP1; betaTrCP; BETA-TRCP
  [Similar Products]
NCBI Protein Information
F-box/WD repeat-containing protein 1A
UniProt Protein Name
F-box/WD repeat-containing protein 1A
UniProt Synonym Protein Names
E3RSIkappaB; Epididymis tissue protein Li 2a; F-box and WD repeats protein beta-TrCP; pIkappaBalpha-E3 receptor subunit
UniProt Gene Name
UniProt Synonym Gene Names
BTRCP; FBW1A; FBXW1A  [Similar Products]
UniProt Entry Name
FBW1A_HUMAN
NCBI Summary for BTRC
This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbws class; in addition to an F-box, this protein contains multiple WD-40 repeats. The encoded protein mediates degradation of CD4 via its interaction with HIV-1 Vpu. It has also been shown to ubiquitinate phosphorylated NFKBIA (nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha), targeting it for degradation and thus activating nuclear factor kappa-B. Alternatively spliced transcript variants have been described. A related pseudogene exists in chromosome 6. [provided by RefSeq, Mar 2012]
UniProt Comments for BTRC
BTRC: a substrate-recognition component of the SCF (SKP1-CUL1-F-box protein) ubiquitin ligase complex, which mediates the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription. Regulates the stability of CTNNB1 and participates in Wnt signaling. Interacts directly with SKP1 in the SCF complex. Interacts specifically with phosphorylated CTNNB1 and NFKBIA, ubiquitination substrates. Two alternatively spliced isoforms have been described.

Protein type: Motility/polarity/chemotaxis; Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 10q24.32

Cellular Component: cytosol; nucleoplasm; SCF ubiquitin ligase complex

Molecular Function: beta-catenin binding; protein binding; ubiquitin-protein ligase activity

Biological Process: G2/M transition of mitotic cell cycle; negative regulation of smoothened signaling pathway; negative regulation of transcription factor activity; negative regulation of transcription, DNA-dependent; positive regulation of circadian rhythm; positive regulation of proteolysis; positive regulation of transcription, DNA-dependent; positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; proteasomal ubiquitin-dependent protein catabolic process; protein amino acid dephosphorylation; protein destabilization; protein polyubiquitination; protein ubiquitination; regulation of circadian rhythm; SCF-dependent proteasomal ubiquitin-dependent protein catabolic process; signal transduction; stimulatory C-type lectin receptor signaling pathway; stress-activated MAPK cascade; T cell receptor signaling pathway; ubiquitin-dependent protein catabolic process; Wnt receptor signaling pathway
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000