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anti-CBL antibody :: Rabbit anti-Human c-Cbl Polyclonal Antibody

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Catalog # MBS624327
Unit / Price
  0.1 mL  /  $590 +1 FREE 8GB USB
anti-CBL antibody
Product Name

c-Cbl, Polyclonal Antibody

Also Known As

c-Cbl, phosphorylated (Tyr731)

Product Synonym Names
Anti -c-Cbl, phosphorylated (Tyr731)
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Chromosome Location
Chromosome: 11; NC_000011.9 (119076990..119178859). Location: 11q23.3
OMIM
165360
3D Structure
ModBase 3D Structure for P22681
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Specificity
Detects overexpressed levels of Tyr731 phosphorylated c-Cbl. The antibody does not crossreact with other related tyrosine-phosphorylated proteins.
Purity/Purification
Affinity Purified
Purified by protein A and peptide affinity chromatography.
Form/Format
Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, BSA, 50% glycerol.
Immunogen
Synthetic peptide (KLH coupled) corresponding to residues surrounding Tyr731 of human c-Cbl.
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Other Notes
Small volumes of anti-CBL antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-CBL antibody
C-Cbl proto-oncogene is a ubiquitously expressed cytoplasmic adaptor protein that is especially predominant in hematopoietic cells (1,2). c-Cbl is rapidly tyrosine phosphorylated in response to stimulation of a variety of cell-surface receptors and becomes associated with a number of intracellular signaling molecules such as protein tyrosine kinases, phosphatidylinositol 3 kinase, Crk and 14-3-3 proteins (3,4). c-Cbl possesses a highly conserved N-terminal phosphotyrosine binding domain (TKB), a C3HC4 RING finger motif. TKB recognizes phosphorylated tyrosines on activated receptor tyrosine kinases (RTKs) as well as other nonreceptor tyrosine kinases. Its RING finger domain recruits ubiquitin-conjugating enzymes. These two domains are primarily responsible for c-Cbl proteins functioning as ubiquitin protein ligases and downregulators of RTKs (3). c-Cbl protein in human cancer tissues is frequently tyrosine phosphorylated in a tumor-specific manner (5). Phosphorylation of Tyr731 of c-Cbl provides a docking site for downstream signaling components such as p85 and Fyn (6).
Product Categories/Family for anti-CBL antibody
Applications Tested/Suitable for anti-CBL antibody
Western Blot (WB)
Application Notes for anti-CBL antibody
Suitable for use in Western Blotting.
Dilution: Western Blot: 1:1000
NCBI/Uniprot data below describe general gene information for CBL. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
99,633 Da[Similar Products]
NCBI Official Full Name
c-cbl
NCBI Official Synonym Full Names
Cas-Br-M (murine) ecotropic retroviral transforming sequence
NCBI Official Symbol
NCBI Official Synonym Symbols
CBL2; NSLL; C-CBL; RNF55
  [Similar Products]
NCBI Protein Information
E3 ubiquitin-protein ligase CBL; oncogene CBL2; OTTHUMP00000232087; proto-oncogene c-Cbl; RING finger protein 55; signal transduction protein CBL; casitas B-lineage lymphoma proto-oncogene
UniProt Protein Name
E3 ubiquitin-protein ligase CBL
UniProt Synonym Protein Names
Casitas B-lineage lymphoma proto-oncogene; Proto-oncogene c-Cbl; RING finger protein 55; Signal transduction protein CBL
UniProt Gene Name
UniProt Synonym Gene Names
CBL2; RNF55  [Similar Products]
UniProt Entry Name
CBL_HUMAN
NCBI Summary for CBL
The cbl oncogene was first identified as part of a transforming retrovirus which induces mouse pre-B and pro-B cell lymphomas. As an adaptor protein for receptor protein-tyrosine kinases, it positively regulates receptor protein-tyrosine kinase ubiquitination in a manner dependent upon its variant SH2 and RING finger domains. Ubiquitination of receptor protein-tyrosine kinases terminates signaling by marking active receptors for degradation. [provided by RefSeq]
UniProt Comments for CBL
CBL: an adapter protein that functions as a negative regulator of many signaling pathways that start from receptors at the cell surface. Acts as an E3 ubiquitin-protein ligase, which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, and then transfers it to substrates promoting their degradation by the proteasome. Recognizes activated receptor tyrosine kinases, including PDGFA, EGF and CSF1, and terminates signaling. Participates in signal transduction in hematopoietic cells.

Protein type: EC 6.3.2.-; Ubiquitin ligase; Motility/polarity/chemotaxis; Ligase; Oncoprotein; Ubiquitin conjugating system; Adaptor/scaffold; EC 6.3.2.19

Chromosomal Location of Human Ortholog: 11q23.3

Cellular Component: cytoplasm; plasma membrane; flotillin complex; cytosol; nucleus

Molecular Function: signal transducer activity; protein binding; ephrin receptor binding; zinc ion binding; phosphotyrosine binding; ubiquitin-protein ligase activity; calcium ion binding; transcription factor activity; SH3 domain binding; ligase activity

Biological Process: negative regulation of epidermal growth factor receptor signaling pathway; epidermal growth factor receptor signaling pathway; positive regulation of phosphoinositide 3-kinase cascade; cell surface receptor linked signal transduction; fibroblast growth factor receptor signaling pathway; regulation of transcription, DNA-dependent; transforming growth factor beta receptor signaling pathway; protein ubiquitination; positive regulation of receptor-mediated endocytosis; negative regulation of apoptosis

Disease: Noonan Syndrome-like Disorder With Or Without Juvenile Myelomonocytic Leukemia
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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