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anti-CASP8 antibody :: Rabbit anti-Human Caspase 8 Polyclonal Antibody

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Catalog # MBS2032312 (SPECIAL PROMOTION: Get FREE $5 Starbucks gift card (Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Unit / Price
Scan QR to view Datasheet
  0.01 mg  /  $100 +1 FREE 8GB USB
  0.02 mg  /  $110 +1 FREE 8GB USB
  0.05 mg  /  $150 +1 FREE 8GB USB
  0.1 mg  /  $195 +1 FREE 8GB USB
  0.2 mg  /  $270 +1 FREE 8GB USB
  1 mg  /  $645 +1 FREE 8GB USB
Product Name

Caspase 8 (CASP8), Polyclonal Antibody

Popular Item
Full Product Name

Caspase 8 (CASP8) Polyclonal Antibody

Product Synonym Names
CAP4; FLICE; MACH; MCH5; Cysteinyl Aspartate Specific Proteinases 8; Apoptotic cysteine protease; FADD-homologous ICE/ced-3-like protease; MORT1-associated ced-3 homolog
Matching Pairs
Unconjugated Antibody: Caspase 8 (MBS2032312)
Immunogen: Caspase 8 (MBS2031598)
Matching Pairs
Unconjugated Antibody: Caspase 8 (MBS2032312)
APC-CY7 Conjugated Antibody: Caspase 8 (CASP8) (MBS2048666)
Matching Pairs
Unconjugated Antibody: Caspase 8 (MBS2032312)
PE Conjugated Antibody: Caspase 8 (CASP8) (MBS2048669)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
OMIM
X98172 mRNA
3D Structure
ModBase 3D Structure for Q14790
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Specificity
The antibody is a rabbit polyclonal antibody raised against CASP8. It has been selected for its ability to recognize CASP8 in immunohistochemical staining and western blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4 containing 0.02% NaN3, 50% glycerol.
Concentration
200ug/ml (lot specific)
Immunoglobulin Type
IgG
Conjugate
No Conjugate
Organism Species
Human
Fragment
CASP8 (Leu7~Asp216)
Quality Control
Content: The quality control contains recombinant CASP8 (Leu7~Asp216) disposed in loading buffer.
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
5uL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol,BPB 0.01%, NaN3 0.02%.
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2048672
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
ISO Certification
Manufactured in an ISO 9001:2008 and ISO 13485:2003 Certified Laboratory.
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.
Other Notes
Small volumes of anti-CASP8 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Product Categories/Family for anti-CASP8 antibody
Applications Tested/Suitable for anti-CASP8 antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA)
Application Notes for anti-CASP8 antibody
Western Blot: 1:50-400
Immunohistochemistry in formalin fixed frozen section: 1:50-500
Enzyme-linked Immunosorbent Assay: 1:100-200

Western Blot (WB) of anti-CASP8 antibody
Western Blot: Sample: Lane1: Human Liver Tissue; Lane2: Human HepG2 Cells; Lane3: Human Hela Cells.
anti-CASP8 antibody Western Blot (WB) (WB) image
Western Blot (WB) of anti-CASP8 antibody
Western Blot: Sample: Recombinant CASP8, Human.
anti-CASP8 antibody Western Blot (WB) (WB) image
Immunohistochemistry (IHC) of anti-CASP8 antibody
FITC staining on IHC-P Simple: Hela cells)
anti-CASP8 antibody Immunohistochemistry (IHC) (IHC) image
Immunohistochemistry (IHC) of anti-CASP8 antibody
DAB staining on IHC-P; Samples: Human Pancreas Cancer Tissue
anti-CASP8 antibody Immunohistochemistry (IHC) (IHC) image
Immunohistochemistry (IHC) of anti-CASP8 antibody
DAB staining on IHC-P; Samples: Human Rectum Cancer Tissue
anti-CASP8 antibody Immunohistochemistry (IHC) (IHC) image
Immunohistochemistry (IHC) of anti-CASP8 antibody
DAB staining on IHC-P; Samples: Human Liver Cancer Tissue
anti-CASP8 antibody Immunohistochemistry (IHC) (IHC) image
Immunohistochemistry (IHC) of anti-CASP8 antibody
DAB staining on IHC-P; Samples: Human Breast Cancer Tissue.
anti-CASP8 antibody Immunohistochemistry (IHC) (IHC) image
Knockout Validation of anti-CASP8 antibody
Knockout Validation: Lane 1: Wild-type Hela cell lysate;;Lane 2: CASP8 knockout Hela cell lysate;;Predicted MW: 26-32,46,54-62kDa ;Observed MW: 60kDa;Primary Ab: 3ug/ml Rabbit Anti-Human CASP8 Antibody;Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody;(#MBS2086047)
anti-CASP8 antibody Knockout Validation image
NCBI/Uniprot data below describe general gene information for CASP8. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
61,836 Da
NCBI Official Full Name
caspase-8 isoform C
NCBI Official Synonym Full Names
caspase 8
NCBI Official Symbol
CASP8  [Similar Products]
NCBI Official Synonym Symbols
CAP4; MACH; MCH5; FLICE; ALPS2B; Casp-8
  [Similar Products]
NCBI Protein Information
caspase-8
UniProt Protein Name
Caspase-8
UniProt Synonym Protein Names
Apoptotic cysteine protease; Apoptotic protease Mch-5; CAP4; FADD-homologous ICE/ced-3-like protease; FADD-like ICE; FLICE
Protein Family
UniProt Gene Name
CASP8  [Similar Products]
UniProt Synonym Gene Names
MCH5; CASP-8; FLICE; MACH  [Similar Products]
NCBI Summary for CASP8
This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes composed of a prodomain, a large protease subunit, and a small protease subunit. Activation of caspases requires proteolytic processing at conserved internal aspartic residues to generate a heterodimeric enzyme consisting of the large and small subunits. This protein is involved in the programmed cell death induced by Fas and various apoptotic stimuli. The N-terminal FADD-like death effector domain of this protein suggests that it may interact with Fas-interacting protein FADD. This protein was detected in the insoluble fraction of the affected brain region from Huntington disease patients but not in those from normal controls, which implicated the role in neurodegenerative diseases. Many alternatively spliced transcript variants encoding different isoforms have been described, although not all variants have had their full-length sequences determined. [provided by RefSeq, Jul 2008]
UniProt Comments for CASP8
CASP8: Most upstream protease of the activation cascade of caspases responsible for the TNFRSF6/FAS mediated and TNFRSF1A induced cell death. Binding to the adapter molecule FADD recruits it to either receptor. The resulting aggregate called death- inducing signaling complex (DISC) performs CASP8 proteolytic activation. The active dimeric enzyme is then liberated from the DISC and free to activate downstream apoptotic proteases. Proteolytic fragments of the N-terminal propeptide (termed CAP3, CAP5 and CAP6) are likely retained in the DISC. Cleaves and activates CASP3, CASP4, CASP6, CASP7, CASP9 and CASP10. May participate in the GZMB apoptotic pathways. Cleaves ADPRT. Hydrolyzes the small-molecule substrate, Ac-Asp-Glu-Val-Asp-|-AMC. Likely target for the cowpox virus CRMA death inhibitory protein. Isoform 5, isoform 6, isoform 7 and isoform 8 lack the catalytic site and may interfere with the pro-apoptotic activity of the complex. Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 18 kDa (p18) and a 10 kDa (p10) subunit. Interacts with FADD, CFLAR and PEA15. Isoform 9 interacts at the endoplasmic reticulum with a complex containing BCAP31, BAP29, BCL2 and/or BCL2L1. Interacts with TNFAIP8L2. Interacts with human cytomegalovirus/HHV-5 protein vICA/UL36; this interaction inhibits CASP8 activation. Isoform 1, isoform 5 and isoform 7 are expressed in a wide variety of tissues. Highest expression in peripheral blood leukocytes, spleen, thymus and liver. Barely detectable in brain, testis and skeletal muscle. Belongs to the peptidase C14A family. 9 isoforms of the human protein are produced by alternative splicing.

Protein type: Apoptosis; EC 3.4.22.61; Protease

Chromosomal Location of Human Ortholog: 2q33.1

Cellular Component: CD95 death-inducing signaling complex; cell body; cytoskeleton; cytosol; membrane raft; mitochondrial outer membrane; mitochondrion; neuron projection; nucleoplasm

Molecular Function: cysteine-type endopeptidase activity; cysteine-type peptidase activity; death receptor binding; identical protein binding; peptidase activity; protein binding; protein complex binding; tumor necrosis factor receptor binding; ubiquitin protein ligase binding

Biological Process: activation of cysteine-type endopeptidase activity involved in apoptotic process; activation of cysteine-type endopeptidase activity involved in apoptotic signaling pathway; apoptosis; B cell activation; cell surface receptor signaling pathway; cellular response to organic cyclic compound; death-inducing signaling complex assembly; macrophage differentiation; natural killer cell activation; negative regulation of extrinsic apoptotic signaling pathway via death domain receptors; negative regulation of I-kappaB kinase/NF-kappaB signaling; nucleotide-binding oligomerization domain containing signaling pathway; positive regulation of I-kappaB kinase/NF-kappaB signaling; positive regulation of macrophage differentiation; positive regulation of neuron death; positive regulation of protein insertion into mitochondrial membrane involved in apoptotic signaling pathway; positive regulation of proteolysis; proteolysis; proteolysis involved in cellular protein catabolic process; regulation of extrinsic apoptotic signaling pathway via death domain receptors; regulation of tumor necrosis factor-mediated signaling pathway; response to antibiotic; response to cobalt ion; response to cold; response to estradiol; response to ethanol; response to lipopolysaccharide; response to tumor necrosis factor; T cell activation; toll-like receptor 3 signaling pathway; TRAIL-activated apoptotic signaling pathway

Disease: Breast Cancer; Caspase 8 Deficiency; Hepatocellular Carcinoma; Lung Cancer
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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