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anti-PTPRC antibody :: Rabbit anti-Human CD45 Polyclonal Antibody

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Catalog # MBS175628
Unit / Price
  0.1 mg  /  $315 +1 FREE 8GB USB
Product Name

CD45 (PTPRC), Polyclonal Antibody

Full Product Name

Anti-CD45 antibody

Product Synonym Names
Receptor-type tyrosine-protein phosphatase C; protein tyrosine phosphatase, receptor type, C; B220 antibody; CD 45 antibody; CD45 antibody; CD45 antigen antibody; CD45R antibody; GP180 antibody; L CA antibody; L-CA antibody; LCA antibody; Leukocyte common antigen antibody; loc antibody; Ly-5 antibody; LY5 antibody; Lyt-4 antibody; OTTHUMP00000033813 antibody; OTTHUMP00000033816 antibody; OTTHUMP00000033817 antibody; OTTHUMP00000038574 antibody; Protein tyrosine phosphatase receptor type C antibody; Protein tyrosine phosphatase receptor type c polypeptide antibody; protein tyrosine phosphatase, receptor type, C antibody; PTPRC antibody; PTPRC_HUMAN antibody; Receptor-type tyrosine-protein phosphatase C antibody; SCID due to PTPRC deficiency antibody; T200 antibody; T200 glycoprotein antibody; T200 leukocyte common antigen antibody
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
OMIM
gene 608971
3D Structure
ModBase 3D Structure for P08575
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Purity/Purification
Immunogen affinity purified.
Form/Format
Lyophilized
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human CD45 (1209-1227aa MVSTFEQYQFLYDVIASTY).
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Preparation and Storage
At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Other Notes
Small volumes of anti-PTPRC antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-PTPRC antibody
Description: Rabbit IgG polyclonal antibody for Receptor-type tyrosine-protein phosphatase C(PTPRC) detection. Tested with WB, IHC-P, ICC in Human.
Background: CD45(Cluster of Differentiation 45), also known as PTPRC, LCA or CD45R, is an enzyme that, in humans, is encoded by the PTPRC gene.CD45 is a member of the protein tyrosine phosphatase(PTP) family. CD45 is a major high molecular mass leukocyte cell surface molecule which is also an integral membrane protein tyrosine phosphatase.The cytogenetic location of CD45 is 1q31.3-q32.1. CD45 is especially a prototype for transmembrane protein-tyrosine phosphatase(PTP). Targeted disruption of the CD45 gene leads to enhanced cytokine and interferon receptor-mediated activation of JAKs and STAT proteins. In vitro, CD45 directly dephosphorylates and binds to JAKs. Functionally, CD45 negatively regulates interleukin-3-mediated cellular proliferation, erythropoietin-dependent hematopoiesis, and antiviral responses in vitro and in vivo. CD45 has been best studied in T cells, where it determines T cell receptor signaling thresholds. CD45 is moved into or out of the immunological synapse(IS) membrane microdomain depending on the relative influence of interaction with the extracellular galectin lattice or the intracellular actin cytoskeleton. Galectin interaction can be finetuned by varying usage of the heavily Oglycosylated spliced regions and sialylation of Nlinked carbohydrates.
Applications Tested/Suitable for anti-PTPRC antibody
Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Immunocytochemistry (ICC)

Western Blot (WB) of anti-PTPRC antibody
Anti-CD45 antibody, MBS175628, Western blotting
All lanes: Anti CD45 (MBS175628) at 0.5ug/ml
Lane 1: JURKAT Whole Cell Lysate at 40ug
Lane 2: CEM Whole Cell Lysate at 40ug
Predicted bind size: 147KD
Observed bind size: 147KD
anti-PTPRC antibody Western Blot (WB) (WB) image
Immunohistochemistry (IHC) of anti-PTPRC antibody
Anti-CD45 antibody, MBS175628, IHC(P)
IHC(P): Human Tonsil Tissue
anti-PTPRC antibody Immunohistochemistry (IHC) (IHC) image
Immunocytochemistry (ICC) of anti-PTPRC antibody
Anti-CD45 antibody, MBS175628, ICC
ICC: JURKAT Cell
anti-PTPRC antibody Immunocytochemistry (ICC) (ICC) image
NCBI/Uniprot data below describe general gene information for PTPRC. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
130,898 Da
NCBI Official Full Name
Receptor-type tyrosine-protein phosphatase C
NCBI Official Synonym Full Names
protein tyrosine phosphatase, receptor type, C
NCBI Official Symbol
PTPRC  [Similar Products]
NCBI Official Synonym Symbols
LCA; LY5; B220; CD45; L-CA; T200; CD45R; GP180
  [Similar Products]
NCBI Protein Information
receptor-type tyrosine-protein phosphatase C; CD45 antigen; T200 glycoprotein; T200 leukocyte common antigen; protein tyrosine phosphatase, receptor type, c polypeptide
UniProt Protein Name
Receptor-type tyrosine-protein phosphatase C
UniProt Synonym Protein Names
Leukocyte common antigen; L-CA; T200; CD_antigen: CD45
UniProt Gene Name
PTPRC  [Similar Products]
UniProt Synonym Gene Names
CD45; L-CA  [Similar Products]
UniProt Entry Name
PTPRC_HUMAN
NCBI Summary for PTPRC
The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitosis, and oncogenic transformation. This PTP contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains, and thus is classified as a receptor type PTP. This PTP has been shown to be an essential regulator of T- and B-cell antigen receptor signaling. It functions through either direct interaction with components of the antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signaling. This PTP also suppresses JAK kinases, and thus functions as a regulator of cytokine receptor signaling. Alternatively spliced transcripts variants of this gene, which encode distinct isoforms, have been reported. [provided by RefSeq, Jun 2012]
UniProt Comments for PTPRC
CD45: a receptor type protein tyrosine phosphatase protein. Contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains. The first PTPAse domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Specifically expressed in hematopoietic cells. An essential regulator of T- and B-cell antigen receptor signaling. Functions through either direct interaction with components of the antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signaling. Suppresses JAK kinases, and thus functions as a regulator of cytokine receptor signaling. Four alternatively spliced isoforms have been described.

Protein type: Motility/polarity/chemotaxis; Membrane protein, integral; EC 3.1.3.48; Receptor protein phosphatase, tyrosine

Chromosomal Location of Human Ortholog: 1q31-q32

Cellular Component: focal adhesion; membrane; integral to plasma membrane; plasma membrane; external side of plasma membrane; lipid raft

Molecular Function: protein binding; transmembrane receptor protein tyrosine phosphatase activity; protein tyrosine phosphatase activity; protein kinase binding

Biological Process: B cell proliferation; axon guidance; substrate-bound cell migration, cell release from substrate; regulation of cell cycle; protein amino acid dephosphorylation; T cell receptor signaling pathway; positive regulation of antigen receptor-mediated signaling pathway; bone marrow development; stem cell development; B cell receptor signaling pathway; cell surface receptor linked signal transduction; dephosphorylation; release of sequestered calcium ion into cytosol; positive regulation of protein kinase activity; negative regulation of T cell mediated cytotoxicity; positive regulation of B cell proliferation; negative regulation of protein kinase activity; hemopoietic progenitor cell differentiation; negative regulation of cytokine and chemokine mediated signaling pathway; positive regulation of T cell proliferation; immunoglobulin biosynthetic process; defense response to virus; T cell differentiation

Disease: Hepatitis C Virus, Susceptibility To; Severe Combined Immunodeficiency, Autosomal Recessive, T Cell-negative, B Cell-positive, Nk Cell-positive
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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