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anti-CHD4 antibody :: Rabbit anti-Human CHD4 Polyclonal Antibody

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Catalog # MBS240754
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Immunohistochemistry (IHC)
Product Name

CHD4, Polyclonal Antibody

Also Known As

Rabbit Polyclonal to Human CHD4

Product Synonym Names
Anti-CHD4 Antibody IHC-plus; CHD4; ATP-dependent helicase CHD4; CHD-4; Mi-2b; Mi2-BETA; Human CHD4
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
1912
OMIM
603277
3D Structure
ModBase 3D Structure for Q14839
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human
Purity/Purification
Protein G Purified
Form/Format
PBS, 0.09% sodium azide.
Target Species
Human
Immunogen Description
Synthetic peptide from human CHD4.
Immunogen Type
Synthetic peptide
Immunogen
CHD4 antibody was raised against synthetic peptide from human CHD4.
Preparation and Storage
Long term: -20 degree C; Short term: +4 degree C. Avoid repeat freeze-thaw cycles.
Other Notes
Small volumes of anti-CHD4 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-CHD4 antibody
The CHD family of proteins is characterized by the presence of chromo (chromatin organization modifier) domains and SNF2-related helicase/ATPase domains. Patients with dermatomyositis develop antibodies against the nuclear antigen chromodomain helicase DNA binding protein 4. The protein exists in a complex containing histone deacetylase and nucleosome remodeling activities, suggesting a role for chromatin reorganization in cancer metastasis.
Applications Tested/Suitable for anti-CHD4 antibody
Immunohistochemistry (IHC - Paraffin), ELISA (EIA)
Application Notes for anti-CHD4 antibody
ELISA (1:000 - 1:1000), IHC-P (20 ug/ml)
Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antib...

Immunohistochemistry (IHC) of anti-CHD4 antibody
Anti-CHD4 antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 20 ug/ml.
anti-CHD4 antibody Immunohistochemistry (IHC) (IHC) image
NCBI/Uniprot data below describe general gene information for CHD4. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
220,848 Da
NCBI Official Full Name
chromodomain-helicase-DNA-binding protein 4 isoform 1
NCBI Official Synonym Full Names
chromodomain helicase DNA binding protein 4
NCBI Official Symbol
NCBI Official Synonym Symbols
CHD-4; Mi-2b; Mi2-BETA
  [Similar Products]
NCBI Protein Information
chromodomain-helicase-DNA-binding protein 4; ATP-dependent helicase CHD4; Mi-2 autoantigen 218 kDa protein
UniProt Protein Name
Chromodomain-helicase-DNA-binding protein 4
UniProt Synonym Protein Names
ATP-dependent helicase CHD4; Mi-2 autoantigen 218 kDa protein; Mi2-beta
UniProt Gene Name
UniProt Synonym Gene Names
CHD-4  [Similar Products]
UniProt Entry Name
CHD4_HUMAN
NCBI Summary for CHD4
The product of this gene belongs to the SNF2/RAD54 helicase family. It represents the main component of the nucleosome remodeling and deacetylase complex and plays an important role in epigenetic transcriptional repression. Patients with dermatomyositis develop antibodies against this protein. Somatic mutations in this gene are associated with serous endometrial tumors. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2014]
UniProt Comments for CHD4
CHD-4: is an ATP-dependent helicase and a central component of the HDAC-containing nucleosome remodeling and histone deacetylase (NuRD) transcriptional complex. Interacts with MTA1/2, HDAC1, and RbAp46 in the NuRD complex. CHD-4 belongs to the SNF2/RAD54 helicase family of proteins. The NuRD complex, whose subunit composition varies by cell type and in response to physiologic signals, plays central roles in many processes including oncogenesis and metastasis, B cell differentiation, invasive growth of breast cancer, and maintaining the balance between the undifferentiated state and the process of differentiation in embryonic stem cells.

Protein type: DNA-binding; Helicase; EC 3.6.4.12

Chromosomal Location of Human Ortholog: 12p13

Cellular Component: nucleoplasm; centrosome; protein complex; membrane; nuclear chromatin; cytoplasm; NuRD complex; nucleus

Molecular Function: ATP-dependent DNA helicase activity; protein binding; nucleosomal DNA binding; DNA binding; zinc ion binding; microtubule binding; ATP binding

Biological Process: regulation of transcription from RNA polymerase II promoter; establishment and/or maintenance of chromatin architecture; transcription, DNA-dependent; ATP-dependent chromatin remodeling; spindle assembly; DNA duplex unwinding
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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