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anti-CHRNE antibody :: Rabbit anti-Human CHRNE Polyclonal Antibody

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Catalog # MBS7045408
Unit / Price
  0.05 mL  /  $120 +1 FREE 8GB USB
  0.1 mL  /  $160 +1 FREE 8GB USB
Product Name

CHRNE, Polyclonal Antibody

Popular Item
Also Known As

CHRNE Antibody

Product Synonym Names
Acetylcholine receptor subunit epsilon; CHRNE; ACHRE
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
493
OMIM
100725
3D Structure
ModBase 3D Structure for Q04844
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid
Species
Human
Immunogen
Recombinant human Acetylcholine receptor subunit epsilon protein (21-239AA)
Conjugate
Non-conjugated
Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Santa Cruz Alternative
Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-1454 / sc-1455 / sc-13999
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Other Notes
Small volumes of anti-CHRNE antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-CHRNE antibody
After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane.
Applications Tested/Suitable for anti-CHRNE antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)

Western Blot (WB) of anti-CHRNE antibody
Western blot
All lanes: CHRNE antibody at 1.87ug/ml
Lane 1: A549 whole cell lysate
Lane 2: Jurkat whole cell lysate
Lane 3: Hela whole cell lysate
Lane 4: HepG-2 whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 55 kDa
Observed band size: 55 kDa

anti-CHRNE antibody Western Blot (WB) (WB) image
Immunohistochemistry (IHC) of anti-CHRNE antibody
Immunohistochemistry of paraffin-embedded human liver tissue MBS7045408 at dilution 1:100
anti-CHRNE antibody Immunohistochemistry (IHC) (IHC) image
Immunohistochemistry (IHC) of anti-CHRNE antibody
Immunohistochemistry of paraffin-embedded human liver cancer MBS7045408 at dilution 1:100
anti-CHRNE antibody Immunohistochemistry (IHC) (IHC) image
NCBI/Uniprot data below describe general gene information for CHRNE. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
54,697 Da
NCBI Official Full Name
acetylcholine receptor subunit epsilon
NCBI Official Synonym Full Names
cholinergic receptor nicotinic epsilon subunit
NCBI Official Symbol
CHRNE  [Similar Products]
NCBI Official Synonym Symbols
ACHRE; CMS1D; CMS1E; CMS2A; CMS4A; CMS4B; CMS4C; FCCMS; SCCMS
  [Similar Products]
NCBI Protein Information
acetylcholine receptor subunit epsilon
UniProt Protein Name
Acetylcholine receptor subunit epsilon
Protein Family
UniProt Gene Name
CHRNE  [Similar Products]
UniProt Synonym Gene Names
ACHRE  [Similar Products]
UniProt Entry Name
ACHE_HUMAN
NCBI Summary for CHRNE
Acetylcholine receptors at mature mammalian neuromuscular junctions are pentameric protein complexes composed of four subunits in the ratio of two alpha subunits to one beta, one epsilon, and one delta subunit. The acetylcholine receptor changes subunit composition shortly after birth when the epsilon subunit replaces the gamma subunit seen in embryonic receptors. Mutations in the epsilon subunit are associated with congenital myasthenic syndrome. [provided by RefSeq, Sep 2009]
UniProt Comments for CHRNE
nAChRE: After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane. The muscle AChR is the major target antigen in the autoimmune disease myasthenia gravis. Myasthenia gravis is characterized by sporadic muscular fatigability and weakness, occurring chiefly in muscles innervated by cranial nerves, and characteristically improved by cholinesterase-inhibiting drugs. Defects in CHRNE are a cause of congenital myasthenic syndrome slow-channel type (SCCMS). SCCMS is the most common congenital myasthenic syndrome. Congenital myasthenic syndromes are characterized by muscle weakness affecting the axial and limb muscles (with hypotonia in early-onset forms), the ocular muscles (leading to ptosis and ophthalmoplegia), and the facial and bulbar musculature (affecting sucking and swallowing, and leading to dysphonia). The symptoms fluctuate and worsen with physical effort. SCCMS is caused by kinetic abnormalities of the AChR, resulting in prolonged endplate currents and prolonged AChR channel opening episodes. Defects in CHRNE are a cause of congenital myasthenic syndrome fast-channel type (FCCMS). FCCMS is a congenital myasthenic syndrome characterized by kinetic abnormalities of the AChR. In most cases, FCCMS is due to mutations that decrease activity of the AChR by slowing the rate of opening of the receptor channel, speeding the rate of closure of the channel, or decreasing the number of openings of the channel during ACh occupancy. The result is failure to achieve threshold depolarization of the endplate and consequent failure to fire an action potential. Defects in CHRNE are a cause of congenital myasthenic syndrome with acetylcholine receptor deficiency (CMS-ACHRD). CMS-ACHRD is a postsynaptic congenital myasthenic syndrome. Mutations underlying AChR deficiency cause a 'loss of function' and show recessive inheritance. Belongs to the ligand-gated ion channel (TC 1.A.9) family. Acetylcholine receptor (TC 1.A.9.1) subfamily. Epsilon/CHRNE sub-subfamily.

Protein type: Channel, ligand-gated; Membrane protein, integral; Membrane protein, multi-pass; Channel, cation

Chromosomal Location of Human Ortholog: 17p13.2

Cellular Component: integral to plasma membrane; plasma membrane

Molecular Function: acetylcholine binding; acetylcholine receptor activity; cation transmembrane transporter activity; ligand-gated ion channel activity

Biological Process: muscle contraction; neuromuscular synaptic transmission; response to nicotine; signal transduction; synaptic transmission, cholinergic; transport

Disease: Myasthenic Syndrome, Congenital, 4a, Slow-channel; Myasthenic Syndrome, Congenital, 4b, Fast-channel; Myasthenic Syndrome, Congenital, Associated With Acetylcholine Receptor Deficiency
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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Organs/Tissues associated with anti-CHRNE antibody
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