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anti-F8 antibody :: Rabbit anti-Human Coagulation Factor VIII (F8) Polyclonal Antibody

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Catalog # MBS2002060 (SPECIAL PROMOTION: Get FREE $5 Starbucks gift card (Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Unit / Price
  0.01 mg  /  $105 +1 FREE 8GB USB
  0.02 mg  /  $125 +1 FREE 8GB USB
  0.05 mg  /  $170 +1 FREE 8GB USB
  0.1 mg  /  $220 +1 FREE 8GB USB
  0.2 mg  /  $320 +1 FREE 8GB USB
  1 mg  /  $760 +1 FREE 8GB USB
Product Name

Coagulation Factor VIII (F8), Polyclonal Antibody

Popular Item
Also Known As

Polyclonal Antibody to Coagulation Factor VIII (F8)

Product Gene Name
Matching Pairs
Matching Pairs
Matching Pairs
Matching Pairs
Matching Pairs
Matching Pairs
Matching Pairs
Unconjugated Antibody: Coagulation Factor VIII (F8) (MBS2002060)
APC-CY7 Conjugated Secondary Antibody: Immunoglobulin G (MBS2090675)
Matching Pairs
Unconjugated Antibody: Coagulation Factor VIII (F8) (MBS2002060)
Unconjugated Secondary Antibody: Immunoglobulin G (MBS2090678)
Matching Pairs
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
2319
Immunogen Sequence
Antigen: The target protein is fused with N-terminal His-Tag and its sequence is listed below.
MGHHHHHHSG SEF-GRQV TVQEFALFFT IFDETKSWYF TENMERNCRA PCNIQMEDPT FKENYRFHAI NGYIMDTLPG LVMAQDQRIR WYLLSMGSNE NIHSIHFSGH VFTVRKKEEY
OMIM
M14113 mRNA
3D Structure
ModBase 3D Structure for P00451
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Specificity
The antibody is a rabbit polyclonal antibody raised against F8. It has been selected for its ability to recognize F8 in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
Concentration
200ug/ml (lot specific)
Immunogen
Recombinant F8 (Gly1887~Leu2044) expressed in E.coli.
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2060195
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Other Notes
Small volumes of anti-F8 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-F8 antibody
Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
Application Notes for anti-F8 antibody
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200

Western Blot (WB) of anti-F8 antibody
Western Blot: Sample: Recombinant protein.
anti-F8 antibody Western Blot (WB) (WB) image
Immunohistochemistry (IHC) of anti-F8 antibody
DAB staining on fromalin fixed paraffin- embedded glioma tissue)
anti-F8 antibody Immunohistochemistry (IHC) (IHC) image
Immunohistochemistry (IHC) of anti-F8 antibody
DAB staining on IHC-P; Samples: Human Cerebrum Tissue)
anti-F8 antibody Immunohistochemistry (IHC) (IHC) image
NCBI/Uniprot data below describe general gene information for F8. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
24,641 Da
NCBI Official Full Name
coagulation factor VIII isoform a preproprotein
NCBI Official Synonym Full Names
coagulation factor VIII
NCBI Official Symbol
NCBI Official Synonym Symbols
AHF; F8B; F8C; HEMA; FVIII; DXS1253E
  [Similar Products]
NCBI Protein Information
coagulation factor VIII
UniProt Protein Name
Coagulation factor VIII
UniProt Synonym Protein Names
Antihemophilic factor; AHF
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
F8C; AHF  [Similar Products]
NCBI Summary for F8
This gene encodes coagulation factor VIII, which participates in the intrinsic pathway of blood coagulation; factor VIII is a cofactor for factor IXa which, in the presence of Ca+2 and phospholipids, converts factor X to the activated form Xa. This gene produces two alternatively spliced transcripts. Transcript variant 1 encodes a large glycoprotein, isoform a, which circulates in plasma and associates with von Willebrand factor in a noncovalent complex. This protein undergoes multiple cleavage events. Transcript variant 2 encodes a putative small protein, isoform b, which consists primarily of the phospholipid binding domain of factor VIIIc. This binding domain is essential for coagulant activity. Defects in this gene results in hemophilia A, a common recessive X-linked coagulation disorder. [provided by RefSeq, Jul 2008]
UniProt Comments for F8
F8: Factor VIII, along with calcium and phospholipid, acts as a cofactor for factor IXa when it converts factor X to the activated form, factor Xa. Defects in F8 are the cause of hemophilia A (HEMA). A disorder of blood coagulation characterized by a permanent tendency to hemorrhage. About 50% of patients have severe hemophilia resulting in frequent spontaneous bleeding into joints, muscles and internal organs. Less severe forms are characterized by bleeding after trauma or surgery. Of particular interest for the understanding of the function of F8 is the category of CRM (cross-reacting material) positive patients (approximately 5%) that have considerable amount of F8 in their plasma (at least 30% of normal), but the protein is non- functional; i.e. the F8 activity is much less than the plasma protein level. CRM-reduced is another category of patients in which the F8C antigen and activity are reduced to approximately the same level. Most mutations are CRM negative, and probably affect the folding and stability of the protein. Belongs to the multicopper oxidase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: Xq28

Cellular Component: COPII-coated ER to Golgi transport vesicle; endoplasmic reticulum lumen; endoplasmic reticulum-Golgi intermediate compartment membrane; extracellular region; extracellular space; Golgi membrane; plasma membrane

Molecular Function: copper ion binding; oxidoreductase activity; protein binding

Biological Process: acute-phase response; blood coagulation; blood coagulation, intrinsic pathway; COPII vesicle coating; ER to Golgi vesicle-mediated transport; platelet activation; platelet degranulation

Disease: Hemophilia A
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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