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anti-COL3A1 antibody :: Rabbit anti-Human COLLAGEN III Polyclonal Antibody

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Catalog # MBS220757
Unit / Price
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  0.5 mL  /  $550 +1 FREE 8GB USB
anti-COL3A1 antibody
Product Name

COLLAGEN III (COL3A1), Polyclonal Antibody

Full Product Name

RABBIT ANTI HUMAN COLLAGEN III

Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Chromosome Location
Chromosome: 2; NC_000002.11 (189839099..189877472). Location: 2q31
OMIM
100070
3D Structure
ModBase 3D Structure for P02461
Clonality
Polyclonal
Isotype
Polyclonal IgG
Host
Rabbit
Species Reactivity
Human
Specificity
COLLAGEN III
This item is specific for human collagen type III. It has shown the following cross-reactivities.

Human collagen type I<15%
Human collagen type IV<5%
Human collagen type II and V<1%

Human plasma proteins do not interfere with binding of the antibody to collagen.
Form/Format
Purified (Purified IgG - lyophilised)
Concentration
0.1 mg/ml after reconstitution (lot specific)
Immunogen
Collagen III from human placenta
Antiserum Preparation
Antiserum to native and denatured human collagen type III was raised by repeated immunisation of rabbits with highly purified antigen. This item has been cross-absorbed with immobilised human collagen types I, IV and V and with human blood plasma proteins.
Preservative Stabilizers
1% Dextran
1% Mannitol
Reconstitution
Reconstitute with 0.5 ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. We recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
Buffer Solution
Phosphate/Borate buffered saline.
Preparation and Storage
Prior to reconstitution store at +4 degree C.
After reconstitution store at -20 degree C.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody.
Shelf Life: 12 months from date of reconstitution.
Other Notes
Small volumes of anti-COL3A1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-COL3A1 antibody
Immunohistology - Frozen (IHC), ELISA (EIA), Immunohistology - Paraffin (IHC)
Application Notes for anti-COL3A1 antibody
Immunohistology - Paraffin: Min Dilution: 1/10
Max Dilution: 1/40

ELISA: Min Dilution: 1/2000
Max Dilution: 1/8000
NCBI/Uniprot data below describe general gene information for COL3A1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
138,564 Da[Similar Products]
NCBI Official Full Name
collagen alpha-1(III) chain preproprotein
NCBI Official Synonym Full Names
collagen, type III, alpha 1
NCBI Official Symbol
COL3A1  [Similar Products]
NCBI Official Synonym Symbols
EDS4A
  [Similar Products]
NCBI Protein Information
collagen alpha-1(III) chain; collagen alpha-1(III) chain; collagen, fetal; alpha1 (III) collagen; Ehlers-Danlos syndrome type IV, autosomal dominant
UniProt Protein Name
Collagen alpha-1(III) chain
Protein Family
UniProt Gene Name
COL3A1  [Similar Products]
UniProt Entry Name
CO3A1_HUMAN
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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