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anti-MMP13 antibody :: Rabbit anti-Human Collagenase 3 Polyclonal Antibody

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Catalog # MBS7005980
Unit / Price
  0.05 mg  /  $160 +1 FREE 8GB USB
  0.1 mg  /  $235 +1 FREE 8GB USB
Product Name

Collagenase 3 (MMP13), Polyclonal Antibody

Popular Item
Full Product Name

Rabbit anti-human Collagenase 3 polyclonal Antibody

Product Synonym Names
Matrix metalloproteinase-13; MMP-13; MMP13
Product Synonym Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
OMIM
250400
3D Structure
ModBase 3D Structure for P45452
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Purity/Purification
Caprylic Acid Ammonium Sulfate Precipitation purified
Form/Format
Liquid
Immunogen
Recombinant human Collagenase 3 protein
Conjugation
Non-conjugated
Storage Buffer
Preservative: 0.03% Proclin 300; Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Santa Cruz Alternative
Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-31811 / sc-31813 / sc-12363 / sc-30073
Preparation and Storage
Shipped at 4 degree C. Upon delivery, aliquot and store at -20 degree C or -80 degree C.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Other Notes
Small volumes of anti-MMP13 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-MMP13 antibody
Degrades collagen type I. Does not act on gelatin or casein. Could have a role in tumoral process.

Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP s are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. This protein cleaves type II collagen more efficiently than types I and III. It may be involved in articular cartilage turnover and cartilage pathophysiology associated with osteoarthritis. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3.
Product Categories/Family for anti-MMP13 antibody
Applications Tested/Suitable for anti-MMP13 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes for anti-MMP13 antibody
Recommended dilution: WB:1:500-2000

Western Blot (WB) of anti-MMP13 antibody
Western blot
All lanes: Collagenase 3 antibody at 6ug/ml+ Jurkat whole cell lysate
Goat polyclonal to rabbit at 1/10000 dilution
Predicted band size: 54 kDa
Observed band size: 54 kDa

anti-MMP13 antibody Western Blot (WB) (WB) image
Western Blot (WB) of anti-MMP13 antibody
Western Blot
Positive WB detected in:PC3 whole cell lysate,Mouse kidney tissue,Mouse lung tissue
All lanes: MMP13 antibody at 2ug/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 54 kDa
Observed band size: 54 kDa

anti-MMP13 antibody Western Blot (WB) (WB) image
Immunohistochemistry (IHC) of anti-MMP13 antibody
Immunohistochemistry of paraffin-embedded human colon cancer using MBS7005980 at dilution of 1:100
anti-MMP13 antibody Immunohistochemistry (IHC) (IHC) image
Immunofluorescence (IF) of anti-MMP13 antibody
Immunofluorescent analysis of PC3 cells using MBS7005980 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
anti-MMP13 antibody Immunofluorescence (IF) image
NCBI/Uniprot data below describe general gene information for MMP13. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
53,820 Da[Similar Products]
NCBI Official Full Name
collagenase 3 preproprotein
NCBI Official Synonym Full Names
matrix metallopeptidase 13
NCBI Official Symbol
MMP13  [Similar Products]
NCBI Official Synonym Symbols
CLG3; MANDP1; MMP-13
  [Similar Products]
NCBI Protein Information
collagenase 3
UniProt Protein Name
Collagenase 3
UniProt Synonym Protein Names
Matrix metalloproteinase-13; MMP-13
Protein Family
UniProt Gene Name
MMP13  [Similar Products]
UniProt Synonym Gene Names
MMP-13  [Similar Products]
UniProt Entry Name
MMP13_HUMAN
NCBI Summary for MMP13
This gene encodes a member of the peptidase M10 family of matrix metalloproteinases (MMPs). Proteins in this family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. The encoded preproprotein is proteolytically processed to generate the mature protease. This protease cleaves type II collagen more efficiently than types I and III. It may be involved in articular cartilage turnover and cartilage pathophysiology associated with osteoarthritis. Mutations in this gene are associated with metaphyseal anadysplasia. This gene is part of a cluster of MMP genes on chromosome 11. [provided by RefSeq, Jan 2016]
UniProt Comments for MMP13
MMP13: Degrades collagen type I. Does not act on gelatin or casein. Could have a role in tumoral process. Defects in MMP13 are the cause of spondyloepimetaphyseal dysplasia Missouri type (SEMD-MO). A bone disease characterized by moderate to severe metaphyseal changes, mild epiphyseal involvement, rhizomelic shortening of the lower limbs with bowing of the femora and/or tibiae, coxa vara, genu varum and pear-shaped vertebrae in childhood. Epimetaphyseal changes improve with age. Defects in MMP13 are the cause of metaphyseal anadysplasia type 1 (MANDP1). Metaphyseal anadysplasia consists of an abnormal bone development characterized by severe skeletal changes that, in contrast with the progressive course of most other skeletal dysplasias, resolve spontaneously with age. Clinical characteristics are evident from the first months of life and include slight shortness of stature and a mild varus deformity of the legs. Patients attain a normal stature in adolescence and show improvement or complete resolution of varus deformity of the legs and rhizomelic micromelia. Belongs to the peptidase M10A family.

Protein type: EC 3.4.24.-; Secreted; Secreted, signal peptide; Protease

Chromosomal Location of Human Ortholog: 11q22.3

Cellular Component: extracellular region; extracellular space; Golgi apparatus; intercellular canaliculus; lysosome; proteinaceous extracellular matrix

Molecular Function: calcium ion binding; calcium-dependent protein binding; collagen binding; fibronectin binding; low-density lipoprotein receptor binding; metalloendopeptidase activity; zinc ion binding

Biological Process: bone mineralization; cellular protein metabolic process; collagen catabolic process; embryonic hindlimb morphogenesis; endochondral ossification; extracellular matrix disassembly; extracellular matrix organization and biogenesis; heart development; luteolysis; osteoblast differentiation; parturition; peptide catabolic process; proteolysis; response to drug; response to estrogen stimulus; response to hypoxia; response to mechanical stimulus

Disease: Spondyloepimetaphyseal Dysplasia, Missouri Type
Precautions
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Disclaimer
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