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anti-COMT antibody :: Goat COMT Polyclonal Antibody

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Catalog # MBS421132
Unit / Price
  0.1 mg  /  $300 +1 FREE 8GB USB
Product Name

COMT, Polyclonal Antibody

Also Known As

Goat anti-COMT (internal) Antibody

Product Synonym Names
Goat Anti-COMT (N Terminus) Antibody; COMT; catechol-O-methyltransferase; COMT antibody; catechol-O-methyltransferase antibody; COMT (internal)
Matching Pairs
Unconjugated Antibody: COMT (MBS421132)
Biotin Conjugated Antibody: COMT (MBS423718)
Antibody/Peptide Pairs
COMT peptide (MBS426652) is used for blocking the activity of COMT antibody (MBS421132)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
271
Immunogen Sequence
QDIIPQLKKK YDVD
OMIM
103780
Clonality
Polyclonal
Host
Goat
Species Reactivity
Tested: Human; Expected from sequence similarity: Human
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (lot specific)
Immunogen
Peptide with sequence C-QDIIPQLKKKYDVD, from the internal region of the protein sequence according to NP_000745.1; NP_009294.1.
Epitope
Internal region
Note
This antibody is expected to recognise both reported isoforms (NP_000745.1 and NP_009294.1)
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Other Notes
Small volumes of anti-COMT antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-COMT antibody
Peptide ELISA (EIA), Western Blot (WB), ELISA (EIA)
Application Notes for anti-COMT antibody
Peptide ELISA: Antibody detection limit dilution 1: 64000.
Western Blot: Approx 28kDa band and faint 25kDa band observed in human testis lysates (calculated MW of 30. 0kDa according to NP_000745.1 and 24.5kDa according to NP_009294.1). Recommended concentration: 0. 03-0.1ug/ml.
Enzyme immunoassay: Sandwich-type ELISA with increasing amount of recombinant COMTcaptured by a rabbit antibody. Recommended reporter concentration: 0.5-2ug/ml.

Western Blot (WB) of anti-COMT antibody
Staining of human testis lysate (35ug protein in RIPA buffer) (0. 03ug/ml). Primary incubation was 1 hour. Detected by chemiluminescence.
anti-COMT antibody Western Blot (WB) (WB) image
Testing Data of anti-COMT antibody
(0.5ug/ml) as the reporter with as the capture rabbit antibody (2.5ug/ml).
anti-COMT antibody Testing Data image
NCBI/Uniprot data below describe general gene information for COMT. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI Related Accession #
Manufactured in an ISO 9001:2015 Certified Laboratory.NP_009294.1[Other Products]
NCBI GenBank Nucleotide #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
24,449 Da
NCBI Official Full Name
catechol O-methyltransferase isoform MB-COMT
NCBI Official Synonym Full Names
catechol-O-methyltransferase
NCBI Official Symbol
NCBI Official Synonym Symbols
HEL-S-98n
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NCBI Protein Information
catechol O-methyltransferase
UniProt Protein Name
Catechol O-methyltransferase
UniProt Gene Name
UniProt Entry Name
COMT_HUMAN
NCBI Summary for COMT
Catechol-O-methyltransferase catalyzes the transfer of a methyl group from S-adenosylmethionine to catecholamines, including the neurotransmitters dopamine, epinephrine, and norepinephrine. This O-methylation results in one of the major degradative pathways of the catecholamine transmitters. In addition to its role in the metabolism of endogenous substances, COMT is important in the metabolism of catechol drugs used in the treatment of hypertension, asthma, and Parkinson disease. COMT is found in two forms in tissues, a soluble form (S-COMT) and a membrane-bound form (MB-COMT). The differences between S-COMT and MB-COMT reside within the N-termini. Several transcript variants are formed through the use of alternative translation initiation sites and promoters. [provided by RefSeq, Sep 2008]
UniProt Comments for COMT
COMT: Catalyzes the O-methylation, and thereby the inactivation, of catecholamine neurotransmitters and catechol hormones. Also shortens the biological half-lives of certain neuroactive drugs, like L-DOPA, alpha-methyl DOPA and isoproterenol. Belongs to the mammalian catechol-O-methyltransferase family. 2 isoforms of the human protein are produced by alternative initiation.

Protein type: Amino Acid Metabolism - tyrosine; Methyltransferase; Membrane protein, integral; EC 2.1.1.6

Chromosomal Location of Human Ortholog: 22q11.21

Cellular Component: axon; cytosol; dendritic spine; integral to membrane; membrane; mitochondrion; plasma membrane; postsynaptic membrane

Molecular Function: catechol O-methyltransferase activity; magnesium ion binding; methyltransferase activity; O-methyltransferase activity; protein binding

Biological Process: cellular response to phosphate starvation; developmental process; dopamine catabolic process; estrogen metabolic process; female pregnancy; learning; methylation; negative regulation of dopamine metabolic process; negative regulation of smooth muscle cell proliferation; neurotransmitter catabolic process; positive regulation of homocysteine metabolic process; regulation of sensory perception of pain; reproductive process in a multicellular organism; response to drug; response to lipopolysaccharide; response to organic cyclic substance; response to pain; short-term memory

Disease: Panic Disorder 1; Schizophrenia
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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