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anti-CDKN1A antibody :: Rabbit anti-Human Cyclin Dependent Kinase Inhibitor 1A (CDKN1A) Polyclonal Antibody

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Catalog # MBS2004323 (SPECIAL PROMOTION: Get FREE $5 Starbucks gift card (Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Unit / Price
Scan QR to view Datasheet
  0.01 mg  /  $110 +1 FREE 8GB USB
  0.02 mg  /  $130 +1 FREE 8GB USB
  0.05 mg  /  $175 +1 FREE 8GB USB
  0.1 mg  /  $225 +1 FREE 8GB USB
  0.2 mg  /  $340 +1 FREE 8GB USB
  1 mg  /  $800 +1 FREE 8GB USB
Product Name

Cyclin Dependent Kinase Inhibitor 1A (CDKN1A), Polyclonal Antibody

Popular Item
Full Product Name

Polyclonal Antibody to Cyclin Dependent Kinase Inhibitor 1A (CDKN1A)

Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence
Antigen: The target protein is fused with N-terminal His-Tag and its sequence is listed below.
MGHHHHHHSG SEF-MSEPAG DVRQ NPCGSKACRR LFGPVDSEQL SRDCDALMAG CIQEARERWN FDFVTETPLE GDFAWERVRG LGLPKLYLPT GPRRGRDELG GGRRPGTSPA LLQGTAEEDH VDLSLSCTLV PRSGEQAEGS PGGPGDSQGR KRRQTSMTDF YHSKRRLIFS KRKP
OMIM
L25610 mRNA
3D Structure
ModBase 3D Structure for P38936
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Specificity
The antibody is a rabbit polyclonal antibody raised against CDKN1A. It has been selected for its ability to recognize CDKN1A in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
Concentration
200ug/ml (lot specific)
Immunogen
Recombinant CDKN1A (Met1~Pro164) expressed in E.coli.
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2074834
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
ISO Certification
Manufactured in an ISO 9001:2008 and ISO 13485:2003 Certified Laboratory.
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.
Other Notes
Small volumes of anti-CDKN1A antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-CDKN1A antibody
Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
Application Notes for anti-CDKN1A antibody
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200

Western Blot (WB) of anti-CDKN1A antibody
Western Blot: Sample: Lane1: Rat Brain Tissue; Lane2: Human Liver Tissue; Lane3: Human Hela Cells; Lane4: Rat Uterus Tissue.
anti-CDKN1A antibody Western Blot (WB) (WB) image
Western Blot (WB) of anti-CDKN1A antibody
Western Blot: Sample: Recombinant CDKN1A, Human.
anti-CDKN1A antibody Western Blot (WB) (WB) image
Immunohistochemistry (IHC) of anti-CDKN1A antibody
DAB staining on IHC-P. Samples: Human Tissue)
anti-CDKN1A antibody Immunohistochemistry (IHC) (IHC) image
NCBI/Uniprot data below describe general gene information for CDKN1A. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
18,119 Da
NCBI Official Full Name
cyclin-dependent kinase inhibitor 1 isoform 1
NCBI Official Synonym Full Names
cyclin dependent kinase inhibitor 1A
NCBI Official Symbol
CDKN1A  [Similar Products]
NCBI Official Synonym Symbols
P21; CIP1; SDI1; WAF1; CAP20; CDKN1; MDA-6; p21CIP1
  [Similar Products]
NCBI Protein Information
cyclin-dependent kinase inhibitor 1
UniProt Protein Name
Cyclin-dependent kinase inhibitor 1
UniProt Synonym Protein Names
CDK-interacting protein 1; Melanoma differentiation-associated protein 6; MDA-6; p21
UniProt Gene Name
CDKN1A  [Similar Products]
UniProt Synonym Gene Names
CAP20; CDKN1; CIP1; MDA6; PIC1; SDI1; WAF1; MDA-6  [Similar Products]
NCBI Summary for CDKN1A
This gene encodes a potent cyclin-dependent kinase inhibitor. The encoded protein binds to and inhibits the activity of cyclin-cyclin-dependent kinase2 or -cyclin-dependent kinase4 complexes, and thus functions as a regulator of cell cycle progression at G1. The expression of this gene is tightly controlled by the tumor suppressor protein p53, through which this protein mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. This protein can interact with proliferating cell nuclear antigen, a DNA polymerase accessory factor, and plays a regulatory role in S phase DNA replication and DNA damage repair. This protein was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of cyclin-dependent kinase2, and may be instrumental in the execution of apoptosis following caspase activation. Mice that lack this gene have the ability to regenerate damaged or missing tissue. Multiple alternatively spliced variants have been found for this gene. [provided by RefSeq, Sep 2015]
UniProt Comments for CDKN1A
p21Cip1: a cell-cycle regulatory protein that Interacts with cyclin-CDK2 and -CDK4, inhibiting cell cycle progression at G1. Its expression is tightly controlled by p53, through which this protein mediates the p53-dependent cell cycle arrest at G1 phase.

Protein type: Cell cycle regulation; Inhibitor

Chromosomal Location of Human Ortholog: 6p21.2

Cellular Component: cyclin-dependent protein kinase holoenzyme complex; cytosol; nuclear body; nucleolus; nucleoplasm; nucleus; perinuclear region of cytoplasm; protein complex

Molecular Function: cyclin binding; cyclin-dependent protein kinase activating kinase activity; cyclin-dependent protein kinase activity; cyclin-dependent protein serine/threonine kinase inhibitor activity; metal ion binding; protein binding; protein complex binding; protein kinase binding; protein kinase inhibitor activity; ubiquitin protein ligase binding

Biological Process: cell cycle arrest; cellular response to amino acid starvation; cellular response to DNA damage stimulus; cellular response to extracellular stimulus; cellular response to gamma radiation; cellular response to heat; cellular response to ionizing radiation; cellular response to UV-B; cellular senescence; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; DNA damage response, signal transduction by p53 class mediator resulting in transcription of p21 class mediator; G1/S transition of mitotic cell cycle; G2/M transition of mitotic cell cycle; intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator; mitotic cell cycle arrest; negative regulation of apoptosis; negative regulation of cell growth; negative regulation of cell proliferation; negative regulation of cyclin-dependent protein kinase activity; negative regulation of cyclin-dependent protein serine/threonine kinase activity; negative regulation of G1/S transition of mitotic cell cycle; negative regulation of gene expression; negative regulation of phosphorylation; organ regeneration; positive regulation of B cell proliferation; positive regulation of cyclin-dependent protein kinase activity; positive regulation of fibroblast proliferation; positive regulation of programmed cell death; positive regulation of protein kinase activity; positive regulation of reactive oxygen species metabolic process; protein kinase B signaling; protein stabilization; Ras protein signal transduction; regulation of cyclin-dependent protein kinase activity; regulation of DNA biosynthetic process; regulation of protein import into nucleus, translocation; regulation of transcription from RNA polymerase II promoter; replicative senescence; response to arsenic; response to corticosterone stimulus; response to drug; response to hyperoxia; response to organonitrogen compound; response to toxin; response to X-ray; stress-induced premature senescence; transcription initiation from RNA polymerase II promoter
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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