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anti-CCNE1 antibody :: Rabbit anti-Human Cyclin E1 Polyclonal Antibody

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Catalog # MBS9601006
Unit / Price
  0.1 mL  /  $255 +1 FREE 8GB USB
  0.2 mL  /  $295 +1 FREE 8GB USB
Product Name

Cyclin E1 (CCNE1), Polyclonal Antibody

Popular Item
Also Known As

Phospho-Cyclin E1 (Thr395) Antibody

Product Synonym Names
CCNE; Ccne1; CCNE1_HUMAN; cyclin E variant ex5del; cyclin E variant ex7del; Cyclin E1; Cyclin Es; Cyclin Et; CyclinE; G1/S specific cyclin E; G1/S-specific cyclin-E1
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
410
3D Structure
ModBase 3D Structure for P24864
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Specificity
Phospho-Cyclin E1 (Thr395) antibody detects endogenous levels of Cyclin E1 only when phosphorylated at Threonine 395
Purity/Purification
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (lot specific)
Immunogen
A synthesized peptide derived from human Cyclin E1 around the phosphorylation site of Threonine 395
Subcellular Location
Nucleus.
Tissue Specificity
Highly expressed in testis and placenta. Low levels in bronchial epithelial cells.
Conjugation
Unconjugated
Epitope
Phospho Thr395
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.
Other Notes
Small volumes of anti-CCNE1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-CCNE1 antibody
Description: A member of the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases.
Function: Essential for the control of the cell cycle at the G1/S (start) transition.
Subunit Structure: Interacts with a member of the CDK2/CDK protein kinases to form a serine/threonine kinase holoenzyme complex. The cyclin subunit imparts substrate specificity to the complex. Found in a complex with CDK2, CABLES1 and CCNA1 (By similarity). Part of a complex consisting of UHRF2, CDK2 and CCNE1. Interacts directly with UHRF2; the interaction ubiquitinates CCNE1 and appears to occur independently of CCNE1 phosphorylation.
Post-translational Modifications: Phosphorylation of both Thr-395 by GSK3 and Ser-399 by CDK2 creates a high affinity degron recognized by FBXW7, and accelerates degradation via the ubiquitin proteasome pathway. Phosphorylation at Thr-77 creates a low affinity degron also recognized by FBXW7. Ubiquitinated by UHRF2; appears to occur independently of phosphorylation.
Similarity: Belongs to the cyclin family. Cyclin E subfamily.
Applications Tested/Suitable for anti-CCNE1 antibody
Western Blot (WB), Immunohistochemisty (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
Application Notes for anti-CCNE1 antibody
WB: 1:500-1:2000
IHC: 1:50-1:200
IF/ICC: 1:100-1:500

Western Blot (WB) of anti-CCNE1 antibody
Western blot analysis of Cyclin E1 phosphorylation expression in Paclitaxel treated HeLa whole cell lysates, The lane on the left is treated with the antigen-specific peptide.
anti-CCNE1 antibody Western Blot (WB) (WB) image
Immunofluorescene (IF) of anti-CCNE1 antibody
MBS9601006 staining MCF7 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
anti-CCNE1 antibody Immunofluorescene (IF) image
NCBI/Uniprot data below describe general gene information for CCNE1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
Observed: 48 kDa
Predicted: 48 kDa
NCBI Official Full Name
G1/S-specific cyclin-E1 isoform 1
NCBI Official Synonym Full Names
cyclin E1
NCBI Official Symbol
CCNE1  [Similar Products]
NCBI Official Synonym Symbols
CCNE; pCCNE1
  [Similar Products]
NCBI Protein Information
G1/S-specific cyclin-E1
UniProt Protein Name
G1/S-specific cyclin-E1
UniProt Gene Name
CCNE1  [Similar Products]
UniProt Synonym Gene Names
NCBI Summary for CCNE1
The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK2, whose activity is required for cell cycle G1/S transition. This protein accumulates at the G1-S phase boundary and is degraded as cells progress through S phase. Overexpression of this gene has been observed in many tumors, which results in chromosome instability, and thus may contribute to tumorigenesis. This protein was found to associate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in cell-cycle regulated histone gene expression and plays a critical role in promoting cell-cycle progression in the absence of pRB. [provided by RefSeq, Apr 2016]
UniProt Comments for CCNE1
Essential for the control of the cell cycle at the G1/S (start) transition.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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