• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

anti-DDIT4 antibody :: Rabbit anti-Human DDIT4 Polyclonal Antibody

Scan QR to view Datasheet
Catalog # MBS7050976
Unit / Price
  0.05 mg  /  $160 +1 FREE 8GB USB
  0.1 mg  /  $235 +1 FREE 8GB USB
Product Name

DDIT4, Polyclonal Antibody

Popular Item
Full Product Name

DDIT4 Antibody

Product Synonym Names
DNA damage-inducible transcript 4 protein; HIF-1 responsive protein RTP801; Protein regulated in development and DNA damage response 1; REDD-1; DDIT4; REDD1; RTP801
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
OMIM
AY090097 mRNA
3D Structure
ModBase 3D Structure for Q9NX09
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Purity/Purification
>95%, Protein G purified
Form/Format
Liquid
Immunogen
Recombinant human DNA damage-inducible transcript 4 protein (1-163AA)
Preservative
0.03% Proclin 300
Constituents
50% Glycerol, 0.01M PBS, PH 7.4
Conjugation
Non-conjugated
Santa Cruz Alternative
Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-46034 / sc-46033 / sc-67051
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Other Notes
Small volumes of anti-DDIT4 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-DDIT4 antibody
Regulates cell growth, proliferation and survival via inhibition of the activity of the mammalian target of rapamycin complex 1 (mTORC1). Inhibition of mTORC1 is mediated by a pathway that involves DDIT4/REDD1, AKT1, the TSC1-TSC2 complex and the GTPase RHEB. Plays an important role in responses to cellular energy levels and cellular stress, including responses to hypoxia and DNA damage. Regulates p53/TP53-mediated apoptosis in response to DNA damage via its effect on mTORC1 activity. Its role in the response to hypoxia depends on the cell type; it mediates mTORC1 inhibition in fibroblasts and thymocytes, but not in hepatocytes (By similarity). Required for mTORC1-mediated defense against viral protein synthesis and virus replication (By similarity). Inhibits neuronal differentiation and neurite outgrowth mediated by NGF via its effect on mTORC1 activity. Required for normal neuron migration during embryonic brain development. Plays a role in neuronal cell death.
Applications Tested/Suitable for anti-DDIT4 antibody
ELISA (EIA), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes for anti-DDIT4 antibody
IHC: 1: 20-1: 200
IF: 1: 50-1: 200

Immunohistochemistry (IHC) of anti-DDIT4 antibody
Immunohistochemistry of paraffin-embedded human liver cancer tissue using MBS7050976 at dilution of 1:100
anti-DDIT4 antibody Immunohistochemistry (IHC) (IHC) image
Immunofluorescence (IF) of anti-DDIT4 antibody
Immunofluorescent analysis of Hela cells using MBS7050976 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
anti-DDIT4 antibody Immunofluorescence (IF) image
NCBI/Uniprot data below describe general gene information for DDIT4. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
25,371 Da
NCBI Official Full Name
DNA damage-inducible transcript 4 protein
NCBI Official Synonym Full Names
DNA damage inducible transcript 4
NCBI Official Symbol
DDIT4  [Similar Products]
NCBI Official Synonym Symbols
Dig2; REDD1; REDD-1
  [Similar Products]
NCBI Protein Information
DNA damage-inducible transcript 4 protein
UniProt Protein Name
DNA damage-inducible transcript 4 protein
UniProt Synonym Protein Names
HIF-1 responsive protein RTP801; Protein regulated in development and DNA damage response 1; REDD-1
UniProt Gene Name
DDIT4  [Similar Products]
UniProt Synonym Gene Names
REDD1; RTP801; REDD-1  [Similar Products]
UniProt Entry Name
DDIT4_HUMAN
UniProt Comments for DDIT4
DDIT4: Inhibits cell growth by regulating the TOR signaling pathway upstream of the TSC1-TSC2 complex and downstream of AKT1. Promotes neuronal cell death. Up-regulated in fibroblasts upon ionizing radiation, via a TP53-dependent pathway. Up-regulated by TP63 in primary keratinocytes, and down-regulated during keratinocyte differentiation. Up-regulated upon DNA alkylation. Up-regulated by amyloid beta-peptide and retinoic acid. Up-regulated by hypoxia, via a PI3K and HIF1A-dependent but TP53/TP63-independent mechanism. Broadly expressed, with lowest levels in brain, skeletal muscle and intestine. Up-regulated in substantia nigra neurons from Parkinson disease patients. Belongs to the DDIT4 family.

Chromosomal Location of Human Ortholog: 10q22.1

Cellular Component: cytoplasm; cytosol; intracellular

Biological Process: brain development; cell proliferation; DNA damage response, signal transduction by p53 class mediator resulting in induction of apoptosis; negative regulation of peptidyl-serine phosphorylation; negative regulation of TOR signaling pathway; nerve growth factor receptor signaling pathway; neuron differentiation; neuron migration; response to hypoxia
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000