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anti-DDT antibody :: Rabbit anti-Human DDT Polyclonal Antibody

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Catalog # MBS175215
Unit / Price
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  0.1 mg  /  $315 +1 FREE 8GB USB
Western Blot (WB)
Product Name

DDT, Polyclonal Antibody

Full Product Name

Anti-human DDT antibody

Product Synonym Names
D-dopachrome decarboxylase; D-dopachrome tautomerase; D dopachrome decarboxylase antibody; D dopachrome tautomerase antibody; D-dopachrome decarboxylase antibody; D-dopachrome tautomerase antibody; DDCT antibody; Ddt antibody; Phenylpyruvate tautomerase II antibody
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
OMIM
602750
3D Structure
ModBase 3D Structure for P30046
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Purity/Purification
Immunogen affinity purified.
Form/Format
Lyophilized
Immunogen
E Coli-derived human DDT recombinant protein (Position: M1-L118).
Contents
Each vial contains 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Preparation and Storage
At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Other Notes
Small volumes of anti-DDT antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-DDT antibody
Description: Rabbit IgG polyclonal antibody for D-dopachrome decarboxylase(DDT) detection. Tested with WB, IHC-P, ICC, ELISA in Human.
Background: DDT, D-dopachrome tautomerization, converts D-dopachrome into 5,6-dihydroxyindole. Northern blot analysis revealed that DDT was expressed as a 0.6-kb mRNA in all tissues tested, with the strongest expression in liver. The DDT gene in human and mouse is identical in exon structure to the MIF gene. Both genes have 2 introns that are located at equivalent positions, relative to a 2-fold repeat in protein structure.the genes for DDT and MIF are closely linked on human chromosome 22 and mouse chromosome 10.
Applications Tested/Suitable for anti-DDT antibody
Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Immunocytochemistry (ICC), ELISA (EIA)

Western Blot (WB) of anti-DDT antibody
Figure. Western blot analysis of DDT using anti- DDT antibody (MBS175215).

Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.

Lane: Recombinant Human DDT Protein 0.5ng

After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti- DDT antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for DDT at approximately 15KD. The expected band size for DDT is at 15KD.
anti-DDT antibody Western Blot (WB) (WB) image
NCBI/Uniprot data below describe general gene information for DDT. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
11,323 Da
NCBI Official Full Name
D-dopachrome decarboxylase
NCBI Official Synonym Full Names
D-dopachrome tautomerase
NCBI Official Symbol
NCBI Official Synonym Symbols
NCBI Protein Information
D-dopachrome decarboxylase; phenylpyruvate tautomerase II
UniProt Protein Name
D-dopachrome decarboxylase
UniProt Synonym Protein Names
D-dopachrome tautomerase; Phenylpyruvate tautomerase II
UniProt Gene Name
UniProt Entry Name
DOPD_HUMAN
NCBI Summary for DDT
D-dopachrome tautomerase converts D-dopachrome into 5,6-dihydroxyindole. The DDT gene is related to the migration inhibitory factor (MIF) in terms of sequence, enzyme activity, and gene structure. DDT and MIF are closely linked on chromosome 22. [provided by RefSeq, Jul 2008]
UniProt Comments for DDT
DDT: Tautomerization of D-dopachrome with decarboxylation to give 5,6-dihydroxyindole (DHI). Belongs to the MIF family.

Protein type: EC 4.1.1.84; Lyase

Chromosomal Location of Human Ortholog: 22q11.23

Cellular Component: cytoplasm

Molecular Function: D-dopachrome decarboxylase activity; dopachrome isomerase activity

Biological Process: melanin biosynthetic process
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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