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anti-Dffb antibody :: Rabbit anti-Human DFF40 Polyclonal Antibody

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Catalog # MBS150231
Unit / Price
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  0.1 mg  /  $375 +1 FREE 8GB USB
Product Name

DFF40 (Dffb), Polyclonal Antibody

Popular Item
Full Product Name

DFF40 Antibody

Product Synonym Names
DFF40; CAD; CPAN; 40kDa; DFF40; Didff; 5730477D02Rik; Cad; DNA fragmentation factor subunit beta; Caspase-activated deoxyribonuclease; CAD; DNA fragmentation factor, beta subunit
Antibody/Peptide Pairs
DFF40 peptide (MBS152764) is used for blocking the activity of DFF40 antibody (MBS150231)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for O54788
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Purity/Purification
DFF40 Antibody is affinity chromatography purified via peptide column.
Form/Format
Liquid
Conjugate
Unconjugated
Immunogen
DFF40 antibody was raised against a 16 amino acid peptide near the amino terminus of human DFF40.
Buffer
DFF40 Antibody is supplied in PBS containing 0.02% sodium azide.
Preparation and Storage
DFF40 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Other Notes
Small volumes of anti-Dffb antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-Dffb antibody
DFF40 Antibody: Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. These death signals finally cause the degradation of chromosomal DNA by activated DNase. A mouse DNase that causes DNA fragmentation was identified recently and designated CAD for caspase activated deoxyribonuclease. The human homologue of mouse CAD was more recently identified by three groups independently and termed CPAN, DFF40, and human CAD, respectively. DFF45/ICAD is the inhibitory protein of DFF40/CAD and forms complex with DFF40/CAD. Upon cleavage of DFF45/ICAD by activated caspase, DFF40/CAD is released and activated and eventually causes the degradation of DNA in the nuclei. Activation of DFF40/CAD, which causes DNA degradation, is the hallmark of apoptotic cell death.
Applications Tested/Suitable for anti-Dffb antibody
ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)
Application Notes for anti-Dffb antibody
DFF40 antibody can be used for detection of DFF40 by Western blot at 0.5 mug/mL. A 40 kDa band can be detected. Antibody can also be used for immunocytochemistry starting at 5 mug/mL. For immunofluorescence start at 20 mug/mL.

User Note: Optimal dilutions for each application to be determined by the researcher.

Positive Control:
MBS151739 K562 Cell Lysate
MBS151729 Jurkat Cell Lysate

Western Blot (WB) of anti-Dffb antibody
Western blot analysis of DFF40 in Jurkat (J) and K562 (K) whole cell lysate with DFF40 antibody at 1:500 dilution.
anti-Dffb antibody Western Blot (WB) (WB) image
Immunocytochemistry (ICC) of anti-Dffb antibody
Immunocytochemistry of DFF40 in Jurkat cells with DFF40 antibody at 5 μg/mL.
anti-Dffb antibody Immunocytochemistry (ICC) (ICC) image
Immunofluorescence (IF) of anti-Dffb antibody
Immunofluorescence of DFF40 in Jurkat cells with DFF40 antibody at 20 μg/mL.
anti-Dffb antibody Immunofluorescence (IF) image
NCBI/Uniprot data below describe general gene information for Dffb. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Related Accession #
Molecular Weight
40 kDa
NCBI Official Full Name
DNA fragmentation factor subunit beta
NCBI Official Synonym Full Names
DNA fragmentation factor, beta subunit
NCBI Official Symbol
NCBI Official Synonym Symbols
CAD; CPAN; 40kDa; DFF40; Didff; 5730477D02Rik
  [Similar Products]
NCBI Protein Information
DNA fragmentation factor subunit beta; DFF-40; caspase-activated DNase; caspase-activated deoxyribonuclease; DNA fragmentation factor 40 kDa subunit; DNase inhibited by DNA fragmentation factor; DNA fragmentation factor, 40 kD, beta subunit
UniProt Protein Name
DNA fragmentation factor subunit beta
UniProt Synonym Protein Names
Caspase-activated deoxyribonuclease; CAD; Caspase-activated DNase; DNA fragmentation factor 40 kDa subunit; DFF-40
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
Cad; CAD; Caspase-activated DNase; DFF-40  [Similar Products]
UniProt Entry Name
DFFB_MOUSE
UniProt Comments for Dffb
DFFB: Nuclease that induces DNA fragmentation and chromatin condensation during apoptosis. Degrades naked DNA and induces apoptotic morphology. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Apoptosis; EC 3.-.-.-; Deoxyribonuclease

Cellular Component: cytoplasm; nuclear chromatin; nucleolus; intracellular; nucleus; cytosol

Molecular Function: protein binding; nuclease activity; enzyme binding; DNA binding; hydrolase activity; deoxyribonuclease activity; GPI-anchor transamidase activity

Biological Process: apoptosis; DNA fragmentation during apoptosis; apoptotic chromosome condensation
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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