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anti-DLD antibody :: Rabbit anti-Human Dihydrolipoyl Dehydrogenase (DLD) Polyclonal Antibody

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Catalog # MBS2015046 (SPECIAL PROMOTION: Get FREE $5 Starbucks gift card (Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Unit / Price
Scan QR to view Datasheet
  0.01 mg  /  $105 +1 FREE 8GB USB
  0.02 mg  /  $125 +1 FREE 8GB USB
  0.05 mg  /  $165 +1 FREE 8GB USB
  0.1 mg  /  $210 +1 FREE 8GB USB
  0.2 mg  /  $310 +1 FREE 8GB USB
  1 mg  /  $725 +1 FREE 8GB USB
Product Name

Dihydrolipoyl Dehydrogenase (DLD), Polyclonal Antibody

Popular Item
Full Product Name

Polyclonal Antibody to Dihydrolipoyl Dehydrogenase (DLD)

Product Gene Name
Matching Pairs
Matching Pairs
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence
Antigen: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- G ATKKSDGKID VSIEAASGGK AEVITCDVLL VCIGRRPFTK NLGLEELGIE LDPRGRIPVN TRFQTKIPNI YAIGDVVAGP MLAHKAEDEG IICVEGMAGG AVHIDYNCVP SVIYTHPEVA WVGKSEEQLK EEGIEYKVGK FPFAANSRAK TNADTDGMVK ILGQKSTDRV LGAHILGPGA GEMVNEAALA LEYGASCEDI
OMIM
J03490 mRNA
3D Structure
ModBase 3D Structure for P09622
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Specificity
The antibody is a rabbit polyclonal antibody raised against DLD. It has been selected for its ability to recognize DLD in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
Concentration
200ug/ml (lot specific)
Immunogen
Recombinant DLD (Gly280~His487) expressed in E.coli.
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2049509
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
ISO Certification
Manufactured in an ISO 9001:2008 and ISO 13485:2003 Certified Laboratory.
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.
Other Notes
Small volumes of anti-DLD antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Product Categories/Family for anti-DLD antibody
Applications Tested/Suitable for anti-DLD antibody
Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
Application Notes for anti-DLD antibody
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200

Western Blot (WB) of anti-DLD antibody
Western Blot: Sample: Mouse Lung lysate; Primary Ab: 1ug/ml Rabbit Anti-Human DLD Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
anti-DLD antibody Western Blot (WB) (WB) image
Western Blot (WB) of anti-DLD antibody
Western Blot: Sample: Recombinant protein.
anti-DLD antibody Western Blot (WB) (WB) image
Immunohistochemistry (IHC) of anti-DLD antibody
DAB staining on fromalin fixed paraffin-embedded Kidney tissue)
anti-DLD antibody Immunohistochemistry (IHC) (IHC) image
Immunohistochemistry (IHC) of anti-DLD antibody
DAB staining on IHC-P; Samples: Human Stomach Tissue)
anti-DLD antibody Immunohistochemistry (IHC) (IHC) image
Knockout Validation of anti-DLD antibody
Knockout Validation: Lane 1: Wild-type HepG2 cell lysate; Lane 2: DLD knockout HepG2 cell lysate; Predicted MW: 54kd Observed MW: 58kd Primary Ab: 1ug/ml Rabbit Anti-Human DLD Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (#MBS2086047)
anti-DLD antibody Knockout Validation image
NCBI/Uniprot data below describe general gene information for DLD. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
49,283 Da
NCBI Official Full Name
dihydrolipoyl dehydrogenase, mitochondrial isoform 1
NCBI Official Synonym Full Names
dihydrolipoamide dehydrogenase
NCBI Official Symbol
NCBI Official Synonym Symbols
E3; LAD; DLDD; DLDH; GCSL; PHE3
  [Similar Products]
NCBI Protein Information
dihydrolipoyl dehydrogenase, mitochondrial
UniProt Protein Name
Dihydrolipoyl dehydrogenase, mitochondrial
UniProt Synonym Protein Names
Dihydrolipoamide dehydrogenase; Glycine cleavage system L protein
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
GCSL; LAD; PHE3  [Similar Products]
NCBI Summary for DLD
This gene encodes a member of the class-I pyridine nucleotide-disulfide oxidoreductase family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. In homodimeric form, the encoded protein functions as a dehydrogenase and is found in several multi-enzyme complexes that regulate energy metabolism. However, as a monomer, this protein can function as a protease. Mutations in this gene have been identified in patients with E3-deficient maple syrup urine disease and lipoamide dehydrogenase deficiency. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]
UniProt Comments for DLD
DLD: a multi-functional mitochondrial enzyme. An enzymatic component of the mitochondrial glycine cleavage system, the pyruvate dehydrogenase complex (PDHC), the alpha-ketoglutarate dehydrogenase complex, and the branched-chain alpha-keto acide dehydrogenase complex. Is the E3 component of the PDHC that catalyzes the overall conversion of pyruvate to acetyl-CoA and CO2. The E3 component has dihydrolipoamide dehydrogenase activity. The PDHC contains 20-30 copies of pyruvate decarboxylase tetramers (2 alpha:2 beta)(E1), 60 copies of dihydrolipoamide acetyltransferase (E2), six homodimers of dihydrolipoamide dehydrogenase (E3), plus E3 binding proteins. Defects in DLD are a cause of maple syrup urine disease (MSUD), characterized by mental and physical retardation, feeding problems and a maple syrup odor to the urine. The keto acids of the branched-chain amino acids are present in the urine, resulting from a block in oxidative decarboxylation. Differentially expressed in the Wernicke's Area from patients with schizophrenia. Inhibited by 5-methoxyindole-2-carboxylic acid (MICA).

Protein type: Amino Acid Metabolism - glycine, serine and threonine; Amino Acid Metabolism - valine, leucine and isoleucine degradation; Carbohydrate Metabolism - citrate (TCA) cycle; Carbohydrate Metabolism - glycolysis and gluconeogenesis; Carbohydrate Metabolism - pyruvate; EC 1.8.1.4; Mitochondrial; Oxidoreductase

Chromosomal Location of Human Ortholog: 7q31.1

Cellular Component: acrosomal matrix; mitochondrial matrix; mitochondrion; myelin sheath; nucleoplasm; oxoglutarate dehydrogenase complex; pyruvate dehydrogenase complex

Molecular Function: dihydrolipoyl dehydrogenase activity; electron transfer activity; FAD binding; lipoamide binding; NAD binding; protein binding; pyruvate dehydrogenase (NAD+) activity

Biological Process: 2-oxoglutarate metabolic process; aging; branched-chain amino acid catabolic process; cell redox homeostasis; cellular nitrogen compound metabolic process; dihydrolipoamide metabolic process; gastrulation; lipoate metabolic process; lysine catabolic process; mitochondrial acetyl-CoA biosynthetic process from pyruvate; mitochondrial electron transport, NADH to ubiquinone; proteolysis; pyruvate metabolic process; regulation of acetyl-CoA biosynthetic process from pyruvate; regulation of membrane potential; sperm capacitation; tricarboxylic acid cycle

Disease: Dihydrolipoamide Dehydrogenase Deficiency
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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