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anti-RRM2B antibody :: Rabbit anti-Human E3 Ribonucleoside-diphosphate reductase subunit M2 B Polyclonal Antibody

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Catalog # MBS1499914
Unit / Price
  0.05 mL  /  $120 +1 FREE 8GB USB
  0.1 mL  /  $160 +1 FREE 8GB USB
Product Name

E3 Ribonucleoside-diphosphate reductase subunit M2 B (RRM2B), Polyclonal Antibody

Popular Item
Also Known As

Rabbit anti-human E3 Ribonucleoside-diphosphate reductase subunit M2 B polyclonal Antibody(RRM2B)

Product Synonym Names
TP53-inducible ribonucleotide reductase M2 B; p53-inducible ribonucleotide reductase small subunit 2-like protein; p53R2; RRM2B; P53R2
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
423
OMIM
604712
3D Structure
ModBase 3D Structure for Q7LG56
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Purity/Purification
Antigen Affinity Purified
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Conjugate
Non-conjugated
Immunogen
Recombinant human Ribonucleoside-diphosphate reductase subunit M2 B protein (1-351aa)
Santa Cruz Alternative
Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-10840 / sc-98384
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Other Notes
Small volumes of anti-RRM2B antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-RRM2B antibody
Plays a pivotal role in cell survival by repairing damaged DNA in a p53/TP53-dependent manner. Supplies deoxyribonucleotides for DNA repair in cells arrested at G1 or G2. Contains an iron-tyrosyl free radical center required for catalysis. Forms an active ribonucleotide reductase (RNR) complex with RRM1 which is expressed both in resting and proliferating cells in response to DNA damage.
Applications Tested/Suitable for anti-RRM2B antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP)
Application Notes for anti-RRM2B antibody
Recommended dilution: WB:1:500-2000
IHC:1:20-1:200

Western Blot (WB) of anti-RRM2B antibody
All lanes: E3 Ribonucleoside-diphosphate reductase subunit M2 B antibody at 6ug/ml
Lane 1:MCF-7 whole cell lysate
Lane 2:Hela whole cell lysate
Lane 3:HepG2 whole cell lysate
Secondary
Goat polyclonal to rabbit at 1/10000 dilution
Predicted band size: 41,35,16,8,5,49 kDa
Observed band size: 41 kDa

anti-RRM2B antibody Western Blot (WB) (WB) image
Immunohistochemistry (IHC) of anti-RRM2B antibody
Immunohistochemistry of paraffin-embedded human lung using MBS1499914 at dilution 1:100
anti-RRM2B antibody Immunohistochemistry (IHC) (IHC) image
Immunoprecipitation (IP) of anti-RRM2B antibody
Immunoprecipitating RRM2B in MCF-7 whole cell lysate
Lane 1: Rabbit monoclonal IgG(1ug)instead of MBS1499914 in MCF-7 whole cell lysate. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the Secondary antibody (1/50000)
Lane 2: MBS1499914(4ug)+ MCF-7 whole cell lysate(500ug)
Lane 3: MCF-7 whole cell lysate (20ug)

anti-RRM2B antibody Immunoprecipitation (IP) (IP) image
NCBI/Uniprot data below describe general gene information for RRM2B. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
48,787 Da
NCBI Official Full Name
ribonucleoside-diphosphate reductase subunit M2 B isoform 2
NCBI Official Synonym Full Names
ribonucleotide reductase regulatory TP53 inducible subunit M2B
NCBI Official Symbol
RRM2B  [Similar Products]
NCBI Official Synonym Symbols
P53R2; MTDPS8A; MTDPS8B
  [Similar Products]
NCBI Protein Information
ribonucleoside-diphosphate reductase subunit M2 B
UniProt Protein Name
Ribonucleoside-diphosphate reductase subunit M2 B
UniProt Synonym Protein Names
TP53-inducible ribonucleotide reductase M2 B; p53-inducible ribonucleotide reductase small subunit 2-like protein; p53R2
UniProt Gene Name
RRM2B  [Similar Products]
UniProt Synonym Gene Names
P53R2; p53R2  [Similar Products]
UniProt Entry Name
RIR2B_HUMAN
NCBI Summary for RRM2B
This gene encodes the small subunit of a p53-inducible ribonucleotide reductase. This heterotetrameric enzyme catalyzes the conversion of ribonucleoside diphosphates to deoxyribonucleoside diphosphates. The product of this reaction is necessary for DNA synthesis. Mutations in this gene have been associated with autosomal recessive mitochondrial DNA depletion syndrome, autosomal dominant progressive external ophthalmoplegia-5, and mitochondrial neurogastrointestinal encephalopathy. Alternatively spliced transcript variants have been described.[provided by RefSeq, Feb 2010]
UniProt Comments for RRM2B
Plays a pivotal role in cell survival by repairing damaged DNA in a p53/TP53-dependent manner. Supplies deoxyribonucleotides for DNA repair in cells arrested at G1 or G2. Contains an iron-tyrosyl free radical center required for catalysis. Forms an active ribonucleotide reductase (RNR) complex with RRM1 which is expressed both in resting and proliferating cells in response to DNA damage.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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