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anti-EIF3D antibody :: Rabbit anti-Human EIF3D Polyclonal Antibody

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Catalog # MBS7006714
Unit / Price
Scan QR to view Datasheet
  0.05 mg  /  $160 +1 FREE 8GB USB
  0.1 mg  /  $235 +1 FREE 8GB USB
Product Name

EIF3D, Polyclonal Antibody

Popular Item
Full Product Name

EIF3D Antibody

Product Synonym Names
Eukaryotic translation initiation factor 3 subunit D; Eukaryotic translation initiation factor 3 subunit 7; EIF3D
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
OMIM
603915
3D Structure
ModBase 3D Structure for O15371
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Purity/Purification
>95%,Protein G purified
Form/Format
Liquid
Immunogen
Recombinant human Eukaryotic translation initiation factor 3 subunit D protein
Conjugation
Non-conjugated
Storage Buffer
Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Santa Cruz Alternative
Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-16372 / sc-31896 / sc-28856
Preparation and Storage
Shipped at 4 degree C. Upon delivery, aliquot and store at -20 degree C or -80 degree C.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Other Notes
Small volumes of anti-EIF3D antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-EIF3D antibody
Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. In case of FCV infection, plays a role in the ribosomal termination-reinitiation event leading to the translation of VP2 (PubMed:18056426).
Applications Tested/Suitable for anti-EIF3D antibody
ELISA (EIA)

Western Blot (WB) of anti-EIF3D antibody
Western Blot
Positive WB detected in: K562 whole cell lysate,HL-60 whole cell lysate,Hela whole cell lysate,MCF-7 whole cell lysate,HepG2 whole cell lysate,Rat liver tissue,Mouse brain tissue,Mouse lung tissue
All lanes: EIF3D antibody at 2ug/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 64,59,63 KDa
Observed band size: 64 KDa

anti-EIF3D antibody Western Blot (WB) (WB) image
Immunohistochemistry (IHC) of anti-EIF3D antibody
IHC image of MBS7006714 diluted at 1:200 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
anti-EIF3D antibody Immunohistochemistry (IHC) (IHC) image
Immunohistochemistry (IHC) of anti-EIF3D antibody
IHC image of MBS7006714 diluted at 1:200 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
anti-EIF3D antibody Immunohistochemistry (IHC) (IHC) image
NCBI/Uniprot data below describe general gene information for EIF3D. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
62,040 Da
NCBI Official Full Name
eukaryotic translation initiation factor 3 subunit D
NCBI Official Synonym Full Names
eukaryotic translation initiation factor 3 subunit D
NCBI Official Symbol
EIF3D  [Similar Products]
NCBI Official Synonym Symbols
EIF3S7; eIF3-p66; eIF3-zeta
  [Similar Products]
NCBI Protein Information
eukaryotic translation initiation factor 3 subunit D
UniProt Protein Name
Eukaryotic translation initiation factor 3 subunit D
UniProt Synonym Protein Names
Eukaryotic translation initiation factor 3 subunit 7
UniProt Gene Name
EIF3D  [Similar Products]
UniProt Synonym Gene Names
eIF3d  [Similar Products]
UniProt Entry Name
EIF3D_HUMAN
NCBI Summary for EIF3D
Eukaryotic translation initiation factor-3 (eIF3), the largest of the eIFs, is a multiprotein complex composed of at least ten nonidentical subunits. The complex binds to the 40S ribosome and helps maintain the 40S and 60S ribosomal subunits in a dissociated state. It is also thought to play a role in the formation of the 40S initiation complex by interacting with the ternary complex of eIF2/GTP/methionyl-tRNA, and by promoting mRNA binding. The protein encoded by this gene is the major RNA binding subunit of the eIF3 complex. [provided by RefSeq, Jul 2008]
UniProt Comments for EIF3D
eIF3-zeta: eukaryotic translation initiation factor 3 subunit 7. Binds to the 40S ribosome and promotes the binding of methionyl-tRNAi and mRNA. Associates with the subunit p170 of eIF-3. eIF-3 is composed of at least 12 different subunits.

Protein type: RNA-binding; Translation initiation; Translation

Chromosomal Location of Human Ortholog: 22q13.1

Cellular Component: cytosol; eukaryotic translation initiation factor 3 complex; membrane

Molecular Function: protein binding; translation initiation factor activity

Biological Process: cellular protein metabolic process; formation of translation initiation complex; formation of translation preinitiation complex; gene expression; regulation of translational initiation; translation; translational initiation
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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