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anti-EIF3K antibody :: Rabbit anti-Human EIF3K Polyclonal Antibody

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Catalog # MBS7045840
Unit / Price
  0.05 mL  /  $120 +1 FREE 8GB USB
  0.1 mL  /  $160 +1 FREE 8GB USB
Product Name

EIF3K, Polyclonal Antibody

Popular Item
Also Known As

EIF3K Antibody

Product Synonym Names
Eukaryotic translation initiation factor 3 subunit K; Eukaryotic translation initiation factor 3 subunit 12; EIF3K
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
218
OMIM
609596
3D Structure
ModBase 3D Structure for Q9UBQ5
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid
Species
Human
Immunogen
Recombinant human Eukaryotic translation initiation factor 3 subunit K protein (1-218AA)
Conjugate
Non-conjugated
Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Santa Cruz Alternative
Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-79476 / sc-79477
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Other Notes
Small volumes of anti-EIF3K antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-EIF3K antibody
Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation.
Applications Tested/Suitable for anti-EIF3K antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP)

Western Blot (WB) of anti-EIF3K antibody
Western blot
All lanes: EIF3K antibody at 1.72ug/ml
Lane 1: Thp-1 whole cell lysate
Lane 2: HT29 whole cell lysate
Lane 3: 293T whole cell lysate
Lane 4: Hela whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 26,25 kDa
Observed band size: 25 kDa

anti-EIF3K antibody Western Blot (WB) (WB) image
Immunohistochemistry (IHC) of anti-EIF3K antibody
Immunohistochemistry of paraffin-embedded human liver cancer using MBS7045840 at dilution 1:100
anti-EIF3K antibody Immunohistochemistry (IHC) (IHC) image
Immunohistochemistry (IHC) of anti-EIF3K antibody
Immunohistochemistry of paraffin-embedded human kidney tissue using MBS7045840 at dilution 1:100
anti-EIF3K antibody Immunohistochemistry (IHC) (IHC) image
Immunoprecipitation (IP) of anti-EIF3K antibody
Immunoprecipitating EIF3K in HeLa whole cell lysate
Lane 1: Rabbit monoclonal IgG(1ug)instead of MBS7045840 in HeLa whole cell lysate. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the Secondary antibody (1/50000)
Lane 2: MBS7045840(4ug)+ HeLa whole cell lysate(500ug)
Lane 3: HeLa whole cell lysate (20ug)

anti-EIF3K antibody Immunoprecipitation (IP) (IP) image
NCBI/Uniprot data below describe general gene information for EIF3K. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
24,484 Da
NCBI Official Full Name
eukaryotic translation initiation factor 3 subunit K isoform 1
NCBI Official Synonym Full Names
eukaryotic translation initiation factor 3 subunit K
NCBI Official Symbol
EIF3K  [Similar Products]
NCBI Official Synonym Symbols
M9; ARG134; PLAC24; PTD001; EIF3S12; HSPC029; MSTP001; PLAC-24; PRO1474; EIF3-p28
  [Similar Products]
NCBI Protein Information
eukaryotic translation initiation factor 3 subunit K
UniProt Protein Name
Eukaryotic translation initiation factor 3 subunit K
UniProt Synonym Protein Names
Eukaryotic translation initiation factor 3 subunit 12
UniProt Gene Name
EIF3K  [Similar Products]
UniProt Synonym Gene Names
eIF3k  [Similar Products]
UniProt Entry Name
EIF3K_HUMAN
NCBI Summary for EIF3K
The 700-kD eukaryotic translation initiation factor-3 (eIF3) is the largest eIF and contains at least 12 subunits, including EIF2S12. eIF3 plays an essential role in translation by binding directly to the 40S ribosomal subunit and promoting formation of the 40S preinitiation complex (Mayeur et al., 2003 [PubMed 14519125]).[supplied by OMIM, Mar 2008]
UniProt Comments for EIF3K
EIF3K: Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. Belongs to the eIF-3 subunit K family.

Protein type: Translation initiation; Translation

Chromosomal Location of Human Ortholog: 19q13.2

Cellular Component: cytoplasm; cytosol; eukaryotic translation initiation factor 3 complex; membrane

Molecular Function: protein binding; translation initiation factor activity

Biological Process: translational initiation
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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