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anti-EIF4A3 antibody :: Rabbit anti-Human EIF4A3 Polyclonal Antibody

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Catalog # MBS7048081
Unit / Price
  0.05 mg  /  $160 +1 FREE 8GB USB
  0.1 mg  /  $235 +1 FREE 8GB USB
Product Name

EIF4A3, Polyclonal Antibody

Popular Item
Full Product Name

EIF4A3 Antibody

Product Synonym Names
Eukaryotic initiation factor 4A-III; ATP-dependent RNA helicase DDX48; ATP-dependent RNA helicase eIF4A-3; DEAD box protein 48; Eukaryotic initiation facto; EIF4A3; DDX48; KIAA0111
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
OMIM
X79538 mRNA
3D Structure
ModBase 3D Structure for P38919
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Purity/Purification
>95%, Protein G purified
Form/Format
Liquid
Immunogen
Recombinant human Eukaryotic initiation factor 4A-III protein (6-166AA)
Preservative
0.03% Proclin 300
Constituents
50% Glycerol, 0.01M PBS, PH 7.4
Conjugation
Non-conjugated
Santa Cruz Alternative
Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-51487 / sc-67369
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Other Notes
Small volumes of anti-EIF4A3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-EIF4A3 antibody
ATP-dependent RNA helicase. Core component of the splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junctions on mRNAs. The EJC is a dynamic structure consisting of core proteins and several peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. The EJC marks the position of the exon-exon junction in the mature mRNA for the gene expression machinery and the core components remain bound to spliced mRNAs throughout all stages of mRNA metabolism thereby influencing downstream processes including nuclear mRNA export, subcellular mRNA localization, translation efficiency and nonsense-mediated mRNA decay (NMD). Its RNA-dependent ATPase and RNA-helicase activities are induced by CASC3, but abolished in presence of the MAGOH-RBM8A heterodimer, thereby trapping the ATP-bound EJC core onto spliced mRNA in a stable conformation. The inhibition of ATPase activity by the MAGOH-RBM8A heterodimer increases the RNA-binding affinity of the EJC. Involved in translational enhancement of spliced mRNAs after formation of the 80S ribosome complex. Binds spliced mRNA in sequence-independent manner, 20-24 nucleotides upstream of mRNA exon-exon junctions. Shows higher affinity for single-stranded RNA in an ATP-bound core EJC complex than after the ATP is hydrolyzed. Involved in the splicing modulation of BCL2L1/Bcl-X (and probably other apoptotic genes); specifically inhibits formation of proapoptotic isoforms such as Bcl-X(S); the function is different from the established EJC assembly. Involved in craniofacial development.
Applications Tested/Suitable for anti-EIF4A3 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes for anti-EIF4A3 antibody
WB: 1: 1000-1: 5000
IHC: 1: 20-1: 200
IF: 1: 50-1: 200

Western Blot (WB) of anti-EIF4A3 antibody
Western Blot
Positive WB detected in:A549 whole cell lysate,MCF-7 whole cell lysate
All lanes: EIF4A3 antibody at 3ug/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 47 kDa
Observed band size: 47 kDa

anti-EIF4A3 antibody Western Blot (WB) (WB) image
Immunohistochemistry (IHC) of anti-EIF4A3 antibody
Immunohistochemistry of paraffin-embedded human gastric cancer using MBS7048081 at dilution of 1:100
anti-EIF4A3 antibody Immunohistochemistry (IHC) (IHC) image
Immunohistochemistry (IHC) of anti-EIF4A3 antibody
Immunofluorescent analysis of MCF-7 cells using MBS7048081 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
anti-EIF4A3 antibody Immunohistochemistry (IHC) (IHC) image
NCBI/Uniprot data below describe general gene information for EIF4A3. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
46,871 Da
NCBI Official Full Name
eukaryotic initiation factor 4A-III
NCBI Official Synonym Full Names
eukaryotic translation initiation factor 4A3
NCBI Official Symbol
EIF4A3  [Similar Products]
NCBI Official Synonym Symbols
RCPS; DDX48; MUK34; NUK34; NMP265; eIF4AIII
  [Similar Products]
NCBI Protein Information
eukaryotic initiation factor 4A-III
UniProt Protein Name
Eukaryotic initiation factor 4A-III
UniProt Synonym Protein Names
ATP-dependent RNA helicase DDX48; ATP-dependent RNA helicase eIF4A-3; DEAD box protein 48; Eukaryotic initiation factor 4A-like NUK-34; Eukaryotic translation initiation factor 4A isoform 3; Nuclear matrix protein 265; NMP 265; hNMP 265
UniProt Gene Name
EIF4A3  [Similar Products]
UniProt Synonym Gene Names
DDX48; KIAA0111; eIF-4A-III; eIF4A-III; NMP 265; hNMP 265  [Similar Products]
UniProt Entry Name
IF4A3_HUMAN
NCBI Summary for EIF4A3
This gene encodes a member of the DEAD box protein family. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. The protein encoded by this gene is a nuclear matrix protein. Its amino acid sequence is highly similar to the amino acid sequences of the translation initiation factors eIF4AI and eIF4AII, two other members of the DEAD box protein family. [provided by RefSeq, Jul 2008]
UniProt Comments for EIF4A3
DDX48: ATP-dependent RNA helicase. Component of a splicing- dependent multiprotein exon junction complex (EJC) deposited at splice junction on mRNAs. The EJC is a dynamic structure consisting of a few core proteins and several more peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. Core components of the EJC, that remains bound to spliced mRNAs throughout all stages of mRNA metabolism, functions to mark the position of the exon-exon junction in the mature mRNA and thereby influences downstream processes of gene expression including mRNA splicing, nuclear mRNA export, subcellular mRNA localization, translation efficiency and nonsense-mediated mRNA decay (NMD). Constitutes at least part of the platform anchoring other EJC proteins to spliced mRNAs. Its RNA-dependent ATPase and RNA-helicase activities are induced by CASC3, but abolished in presence of the MAGOH/RBM8A heterodimer, thereby trapping the ATP- bound EJC core onto spliced mRNA in a stable conformation. The inhibition of ATPase activity by the MAGOH/RBM8A heterodimer increases the RNA-binding affinity of the EJC. Involved in translational enhancement of spliced mRNAs after formation of the 80S ribosome complex. Binds spliced mRNA in sequence-independent manner, 20-24 nucleotides upstream of mRNA exon-exon junctions. Shows higher affinity for single-stranded RNA in an ATP-bound core EJC complex than after the ATP is hydrolyzed. Belongs to the DEAD box helicase family. eIF4A subfamily.

Protein type: EC 3.6.4.13; Helicase; RNA splicing; RNA-binding; Spliceosome

Chromosomal Location of Human Ortholog: 17q25.3

Cellular Component: cytoplasm; cytosol; membrane; nucleoplasm

Molecular Function: ATP binding; ATP-dependent RNA helicase activity; mRNA binding; poly(A) binding; protein binding

Biological Process: embryonic cranial skeleton morphogenesis; mRNA 3'-end processing; mRNA catabolic process, nonsense-mediated decay; mRNA export from nucleus; negative regulation of translation; nuclear mRNA splicing, via spliceosome; poly(A) tail shortening; positive regulation of translation; RNA export from nucleus; RNA secondary structure unwinding; RNA splicing; termination of RNA polymerase II transcription

Disease: Robin Sequence With Cleft Mandible And Limb Anomalies
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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