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anti-ELF3 antibody :: Goat ELF3/ERT/ ESX Polyclonal Antibody

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Catalog # MBS420518
Unit / Price
  0.1 mg  /  $300 +1 FREE 8GB USB
Product Name

ELF3/ERT/ ESX, Polyclonal Antibody

Also Known As

Goat anti-ELF3/ERT/ ESX Antibody

Product Synonym Names
ELF3; ERT; E74-like factor 3 (ets domain transcription factor, epithelial-specific); EPR-1; ESE-1; E74-like factor 3 (ets domain transcription factor); ets domain transcription factor, serine box (epithelial-specific); E74-like factor 3 (ETS domain trans; ELF3 antibody; ERT antibody; E74-like factor 3 (ets domain transcription factor; epithelial-specific) antibody; EPR-1 antibody; ESE-1 antibody; E74-like factor 3 (ets domain transcription factor) antibody; ets domain transcription factor; serine box (epithelial-specific) antibody; E74-like factor 3 (ETS domain transcription factor; serine box; epithelial-specific) antibody; ELF3 / ERT/ ESX
Product Synonym Gene Name
Antibody/Peptide Pairs
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
371
Immunogen Sequence
SGWKEEEVLQ SRN
OMIM
602191
Clonality
Polyclonal
Host
Goat
Species Reactivity
Tested: Human; Expected from sequence similarity: Human
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (lot specific)
Immunogen
Peptide with sequence C-SGWKEEEVLQSRN, from the C Terminus of the protein sequence according to NP_004424.2.
Epitope
C Terminus
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Other Notes
Small volumes of anti-ELF3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-ELF3 antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes for anti-ELF3 antibody
Peptide ELISA: Antibody detection limit dilution 1: 32000.
Western Blot: Approx 48 kDa band observed in NCI-H460 lysate (calculated MW of 41.5kDa according to NP_004424.2). In transfected HEK293 transiently expressing ELF3 a band of approx. 40kDa is observed. This band is not observed in the non-transfected HEK293. Recommended for use at 2-4ug/ml.

Western Blot (WB) of anti-ELF3 antibody
Staining (3ug/ml) of NCI-H460 lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
anti-ELF3 antibody Western Blot (WB) (WB) image
Western Blot (WB) of anti-ELF3 antibody
HEK293 overexpressing ELF3 (RC200631) and probed (mock transfection in first lane), tested by Origene.
anti-ELF3 antibody Western Blot (WB) (WB) image
NCBI/Uniprot data below describe general gene information for ELF3. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
39,357 Da
NCBI Official Full Name
E74-like factor 3 (ets domain transcription factor, epithelial-specific)
NCBI Official Synonym Full Names
E74 like ETS transcription factor 3
NCBI Official Symbol
NCBI Official Synonym Symbols
ERT; ESX; EPR-1; ESE-1
  [Similar Products]
NCBI Protein Information
ETS-related transcription factor Elf-3
UniProt Protein Name
ETS-related transcription factor Elf-3
UniProt Synonym Protein Names
E74-like factor 3; Epithelial-restricted with serine box; Epithelium-restricted Ets protein ESX; Epithelium-specific Ets transcription factor 1; ESE-1
UniProt Gene Name
UniProt Synonym Gene Names
ESE-1  [Similar Products]
UniProt Entry Name
ELF3_HUMAN
UniProt Comments for ELF3
ELF3: Transcriptional activator that binds and transactivates ETS sequences containing the consensus nucleotide core sequence GGA[AT]. Acts synergistically with POU2F3 to transactivate the SPRR2A promoter and with RUNX1 to transactivate the ANGPT1 promoter. Also transactivates collagenase, CCL20, CLND7, FLG, KRT8, NOS2, PTGS2, SPRR2B, TGFBR2 and TGM3 promoters. Represses KRT4 promoter activity. Involved in mediating vascular inflammation. May play an important role in epithelial cell differentiation and tumorigenesis. May be a critical downstream effector of the ERBB2 signaling pathway. May be associated with mammary gland development and involution. Plays an important role in the regulation of transcription with TATA-less promoters in preimplantation embryos, which is essential in preimplantation development. Belongs to the ETS family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding; Motility/polarity/chemotaxis; Transcription factor

Chromosomal Location of Human Ortholog: 1q32.2

Cellular Component: cytoplasm; nucleus

Molecular Function: protein binding; transcription coactivator activity; transcription factor activity

Biological Process: blastocyst development; epidermis development; epithelial cell differentiation; extracellular matrix organization and biogenesis; inflammatory response; mammary gland involution; multicellular organismal development; negative regulation of transcription, DNA-dependent; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; regulation of transcription from RNA polymerase II promoter; transcription from RNA polymerase II promoter
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Pathways associated with anti-ELF3 antibody
 Products by Pathway  Pathway Diagram
 EGFR1 Signaling Pathway antibodies  EGFR1 Signaling Pathway Diagram
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