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anti-ENAM antibody :: Rabbit anti-Human ENAM Polyclonal Antibody

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Catalog # MBS9205809
Unit / Price
  0.08 mL  /  $150 +1 FREE 8GB USB
  0.4 mL  /  $340 +1 FREE 8GB USB
Product Name

ENAM, Polyclonal Antibody

Popular Item
Also Known As

ENAM Antibody (C-term)

Product Synonym Names
Enamelin; ENAM
Antibody/Peptide Pairs
ENAM peptide (MBS9225803) is used for blocking the activity of ENAM antibody (MBS9205809)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Positions
1097-1126
Immunogen Sequence Length
1142
OMIM
104500
3D Structure
ModBase 3D Structure for Q9NRM1
Clonality
Polyclonal
Isotype
Rabbit Ig
Clone Number
RB20947
Host
Rabbit
Species Reactivity
Human
Specificity
This ENAM antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1097-1126 amino acids from the C-terminal region of human ENAM.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Function
Involved in the mineralization and structural organization of enamel. Involved un the extension of enamel during the secretory stage of dental enamel formation.
Cellular Location
Secreted, extracellular space, extracellular matrix {ECO:0000250|UnitProtKB:O97939}
Tissue Location
Expressed in tooth particularly in odontoblast, ameloblast and cementoblast.
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Other Notes
Small volumes of anti-ENAM antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-ENAM antibody
Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC-P), Flow Cytometry (FC/FACS)
Application Notes for anti-ENAM antibody
WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50

Western Blot (WB) of anti-ENAM antibody
ENAM Antibody (C-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the ENAM antibody detected the ENAM protein (arrow).
anti-ENAM antibody Western Blot (WB) (WB) image
Immunohistochemistry (IHC) of anti-ENAM antibody
ENAM antibody(C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ENAM antibody(C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
anti-ENAM antibody Immunohistochemistry (IHC) (IHC) image
Flow Cytometry (FC/FACS) of anti-ENAM antibody
ENAM Antibody (C-term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
anti-ENAM antibody Flow Cytometry (FC/FACS) (FC/FACS) image
NCBI/Uniprot data below describe general gene information for ENAM. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
128785
NCBI Official Full Name
enamelin
NCBI Official Synonym Full Names
enamelin
NCBI Official Symbol
NCBI Official Synonym Symbols
ADAI; AI1C; AIH2
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NCBI Protein Information
enamelin
UniProt Protein Name
Enamelin
Protein Family
UniProt Gene Name
UniProt Entry Name
ENAM_HUMAN
NCBI Summary for ENAM
Dental enamel forms the outer cap of teeth and is the hardest substance found in vertebrates. This gene encodes the largest protein in the enamel matrix of developing teeth. The protein is involved in the mineralization and structural organization of enamel. Defects in this gene result in amelogenesis imperfect type 1C.[provided by RefSeq, Oct 2009]
UniProt Comments for ENAM
enamelin: Involved in the mineralization and structural organization of enamel. Involved in the extension of enamel during the secretory stage of dental enamel formation. Defects in ENAM are the cause of amelogenesis imperfecta type 1B (AI1B). AI1B is an autosomal dominant defect of enamel formation. Clinical manifestations may be variable. Some cases present with generalized enamel hypoplasia resulting in small, smooth, yellow and spaced teeth (smooth hypoplastic AI). Others show horizontal rows of pits, grooves or a hypoplastic area in the enamel (local hypoplastic AI). Defects in ENAM are the cause of amelogenesis imperfecta type 1C (AI1C); also known as amelogenesis imperfecta hypoplastic with or without openbite malocclusion. AI1C is an autosomal recessive defect of dental enamel formation. Teeth show hypoplastic and unmineralized enamel, and a yellow-brown discoloration. Enamel defects can be associated with facial and oral features including vertical dysgnathia and anterior openbite malocclusion.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 4q13.3

Cellular Component: proteinaceous extracellular matrix

Biological Process: biomineral formation

Disease: Amelogenesis Imperfecta, Type Ic; Amelogenesis Imperfecta, Type Ib
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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