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anti-EPB42 antibody :: Rabbit anti-Human Erythrocyte Membrane Protein Band 4.2 Polyclonal Antibody

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Catalog # MBS2027977 (SPECIAL PROMOTION: Get FREE $5 Starbucks gift card (Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Unit / Price
  0.01 mg  /  $110 +1 FREE 8GB USB
  0.02 mg  /  $130 +1 FREE 8GB USB
  0.05 mg  /  $175 +1 FREE 8GB USB
  0.1 mg  /  $225 +1 FREE 8GB USB
  0.2 mg  /  $340 +1 FREE 8GB USB
  1 mg  /  $800 +1 FREE 8GB USB
Product Name

Erythrocyte Membrane Protein Band 4.2 (EPB42), Polyclonal Antibody

Also Known As

Polyclonal Antibody to Erythrocyte Membrane Protein Band 4.2 (EPB42)

Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
691
OMIM
M60298 mRNA
3D Structure
ModBase 3D Structure for P16452
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Specificity
The antibody is a rabbit polyclonal antibody raised against EPB42. It has beenselected for its ability to recognize EPB42 in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Liquid
Concentration
200ug/ml (lot specific)
Fragment
EPB42 (Leu475~Ala686)
Organism Species
Homo sapiens (Human)
Conjugate
No Conjugate
Immunogen
Recombinant EPB42 (Leu475~Ala686) expressed in E Coli.
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2076017
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Other Notes
Small volumes of anti-EPB42 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-EPB42 antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA)
Application Notes for anti-EPB42 antibody
Western blotting: 0.2-2ug/mL;1:250-2500
Immunohistochemistry: 5-20ug/mL;1:25-100
Immunocytochemistry: 5-20ug/mL;1:25-100
Optimal working dilutions must be determined by end user.

Western Blot (WB) of anti-EPB42 antibody
Western Blot: Sample: Recombinant protein.
anti-EPB42 antibody Western Blot (WB) (WB) image
Immunohistochemistry (IHC) of anti-EPB42 antibody
DABstainingonIHC-P.Samples:HumanTissue)
anti-EPB42 antibody Immunohistochemistry (IHC) (IHC) image
NCBI/Uniprot data below describe general gene information for EPB42. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
69,455 Da
NCBI Official Full Name
erythrocyte membrane protein band 4.2 isoform 1
NCBI Official Synonym Full Names
erythrocyte membrane protein band 4.2
NCBI Official Symbol
EPB42  [Similar Products]
NCBI Official Synonym Symbols
PA; SPH5
  [Similar Products]
NCBI Protein Information
erythrocyte membrane protein band 4.2
UniProt Protein Name
Erythrocyte membrane protein band 4.2
UniProt Gene Name
EPB42  [Similar Products]
UniProt Synonym Gene Names
E42P; Erythrocyte protein 4.2; P4.2  [Similar Products]
NCBI Summary for EPB42
Erythrocyte membrane protein band 4.2 is an ATP-binding protein which may regulate the association of protein 3 with ankyrin. It probably has a role in erythrocyte shape and mechanical property regulation. Mutations in the EPB42 gene are associated with recessive spherocytic elliptocytosis and recessively transmitted hereditary hemolytic anemia. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Comments for EPB42
Epb4.2: Probably plays an important role in the regulation of erythrocyte shape and mechanical properties. Defects in EPB42 are the cause of spherocytosis type 5 (SPH5); also known as hereditary spherocytosis type 5 (HS5). Spherocytosis is a hematologic disorder leading to chronic hemolytic anemia and characterized by numerous abnormally shaped erythrocytes which are generally spheroidal. Absence of band 4.2 associated with spur or target erythrocytes has also been reported. Belongs to the transglutaminase superfamily. Transglutaminase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 15q15.2

Cellular Component: cortical cytoskeleton; cytoskeleton; plasma membrane

Molecular Function: ATP binding; protein binding; protein-glutamine gamma-glutamyltransferase activity; structural constituent of cytoskeleton

Biological Process: cell morphogenesis; cytoskeleton organization; erythrocyte maturation; hemoglobin metabolic process; iron ion homeostasis; peptide cross-linking; regulation of cell shape; spleen development

Disease: Spherocytosis, Type 5
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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