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anti-CX3CL1 antibody :: Rabbit anti-Human Fractalkine Polyclonal Antibody

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Catalog # MBS691049
Unit / Price
  0.1 mg  /  $415 +1 FREE 8GB USB
anti-CX3CL1 antibody
Product Name

Fractalkine (CX3CL1), Polyclonal Antibody

Popular Item
Also Known As

Rabbit Anti-Human Fractalkine

Product Synonym Names
Antigen-Affinity Purified Polyclonal Antibodies Anti-human Fractalkine
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
397
Chromosome Location
Chromosome: 16; NC_000016.10 (57372502..57385044). Location: 16q13
OMIM
601880
3D Structure
ModBase 3D Structure for P78423
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Purity/Purification
Antigen-affinity purified
Form/Format
Lyophilized from PBS
Antibody Generation
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant human Fractalkine. Anti-human Fractalkine specific antibody was purified by affinity chromatography employing immobilized human Fractalkine matrix.
Immunogen
Recombinant human Fractalkine
Reconstitution buffer
Water
Reconstitution
Reconstitute the antibody in sterile water to a concentration of 0.1-1.0mg/ml
Buffer
PBS; pH 7.2
Preparation and Storage
The lyophilized antibody is stable for at least 2 years from date of receipt at -20 degree C. The reconstituted antibody is stable for at least two weeks at 2-8 degree C. Frozen aliquots are stable for at least 6 months when stored at -20 degree C. Avoid Repeated Freeze and Thaw Cycle!
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Other Notes
Small volumes of anti-CX3CL1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-CX3CL1 antibody
CX3CL1, also known as Fractalkine, is a type I membrane protein in which a chemokine domain possessing a unique CX3C cysteine motif is tethered on a long mucin-like stalk. It can also be released as a soluble molecule upon proteolysis at a membrane proximal site.
Applications Tested/Suitable for anti-CX3CL1 antibody
ELISA (EIA), Western Blot (WB)
Application Notes for anti-CX3CL1 antibody
Western Blot: To detect human Fractalkine by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant human Fractalkine is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

ELISA: To detect human Fractalkine by direct ELISA (using 100ul/well antibody solution) a concentration of at least 0.5 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant human Fractalkine.
NCBI/Uniprot data below describe general gene information for CX3CL1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
42,203 Da[Similar Products]
NCBI Official Full Name
fractalkine
NCBI Official Synonym Full Names
chemokine (C-X3-C motif) ligand 1
NCBI Official Symbol
CX3CL1  [Similar Products]
NCBI Official Synonym Symbols
NTN; NTT; CXC3; CXC3C; SCYD1; ABCD-3; C3Xkine; fractalkine; neurotactin
  [Similar Products]
NCBI Protein Information
fractalkine; C-X3-C motif chemokine 1; small-inducible cytokine D1; CX3C membrane-anchored chemokine; small inducible cytokine subfamily D (Cys-X3-Cys), member-1; small inducible cytokine subfamily D (Cys-X3-Cys), member 1 (fractalkine, neurotactin)
UniProt Protein Name
Fractalkine
UniProt Synonym Protein Names
C-X3-C motif chemokine 1; CX3C membrane-anchored chemokine; Neurotactin; Small-inducible cytokine D1
Protein Family
UniProt Gene Name
CX3CL1  [Similar Products]
UniProt Synonym Gene Names
FKN; NTT; SCYD1  [Similar Products]
UniProt Entry Name
X3CL1_HUMAN
UniProt Comments for CX3CL1
Function: The soluble form is chemotactic for T-cells and monocytes, but not for neutrophils. The membrane-bound form promotes adhesion of those leukocytes to endothelial cells. May play a role in regulating leukocyte adhesion and migration processes at the endothelium. Binds to CX3CR1. Ref.8

Subunit structure: Monomer. Interacts with pox virus crmD; this inhibits cell migration mediated by CX3CL1. Ref.6 Ref.8

Subcellular location: Cell membrane; Single-pass type I membrane protein. Processed fractalkine: Secreted.

Tissue specificity: Small intestine, colon, testis, prostate, heart, brain, lung, skeletal muscle, kidney and pancreas.

Induction: By TNF and IL1/interleukin-1 in pulmonary endothelial cells and umbilical vein endothelial cells.

Post-translational modification: A soluble short 95 kDa form may be released by proteolytic cleavage from the long membrane-anchored form

By similarity.O-glycosylated with core 1 or possibly core 8 glycans. Ref.5

Sequence similarities: Belongs to the intercrine delta family.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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