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anti-GABARAP antibody :: Rabbit anti-Human Gamma-aminobutyric acid receptor-associated protein Polyclonal Antibody

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Catalog # MBS715566
Unit / Price
  0.05 mg  /  $160 +1 FREE 8GB USB
  0.1 mg  /  $235 +1 FREE 8GB USB
Western Blot (WB)
Product Name

Gamma-aminobutyric acid receptor-associated protein (GABARAP), Polyclonal Antibody

Popular Item
Also Known As

Rabbit anti-human Gamma-aminobutyric acid receptor-associated protein polyclonal Antibody, Biotin conjugated

Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
117
OMIM
605125
3D Structure
ModBase 3D Structure for O95166
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Purity/Purification
Caprylic Acid Ammonium Sulfate Precipitation
Storage Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Conjugate
Biotin
Immunogen
Recombinant human Gamma-aminobutyric acid receptor-associated protein protein
Santa Cruz Alternative
Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-9189 / sc-9190 / sc-28938
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Other Notes
Small volumes of anti-GABARAP antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-GABARAP antibody
May play a role in intracellular transport of GABA(A) receptors and its interaction with the cytoskeleton
Applications Tested/Suitable for anti-GABARAP antibody
ELISA (EIA)

Western Blot (WB) of anti-GABARAP antibody
Western blot All lanes: Casein kinase II subunit beta antibody at at 2 /mlLane 1: EC109 whole cell lysateLane 2: 293T whole cell lysate SecondaryGoat polyclonal to Rabbit IgG at 1/15000 dilution Predicted band size: 13 kDa Observed band size: 80 kDa Why is the actual band size different from the predicted Western blotting is a technique that separates proteins based on size - in general, the smaller the protein the faster it migrates through the gel. However, migration is also affected by other factors and so the actual band size observed may differ from that predicted. Common factors include... · post-translational modification - e.g. phosphorylation, glycosylation etc which increases the size of the protein · post-translation cleavage - e.g. many proteins are synthesized as pro-proteins, and then cleaved to give the active form, e.g. pro-caspases · splice variants - alternative splicing may create different sized proteins from the same gene · relative charge - the composition of amino acids (charged vs non-charged) multimers - e.g. dimerisation of a protein. This is usually prevented in reducing conditions, although strong interactions can result in the appearance of higher bands.
anti-GABARAP antibody Western Blot (WB) (WB) image
NCBI/Uniprot data below describe general gene information for GABARAP. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Related Accession #
Molecular Weight
13,918 Da[Similar Products]
NCBI Official Full Name
gamma-aminobutyric acid receptor-associated protein
NCBI Official Synonym Full Names
GABA(A) receptor-associated protein
NCBI Official Symbol
GABARAP  [Similar Products]
NCBI Official Synonym Symbols
MM46; ATG8A; GABARAP-a
  [Similar Products]
NCBI Protein Information
gamma-aminobutyric acid receptor-associated protein
UniProt Protein Name
Gamma-aminobutyric acid receptor-associated protein
UniProt Synonym Protein Names
GABA(A) receptor-associated protein; MM46
UniProt Gene Name
GABARAP  [Similar Products]
UniProt Synonym Gene Names
FLC3B  [Similar Products]
UniProt Entry Name
GBRAP_HUMAN
NCBI Summary for GABARAP
Gamma-aminobutyric acid A receptors [GABA(A) receptors] are ligand-gated chloride channels that mediate inhibitory neurotransmission. This gene encodes GABA(A) receptor-associated protein, which is highly positively charged in its N-terminus and shares sequence similarity with light chain-3 of microtubule-associated proteins 1A and 1B. This protein clusters neurotransmitter receptors by mediating interaction with the cytoskeleton. [provided by RefSeq, Jul 2008]
UniProt Comments for GABARAP
GABARAP: is a ubiquitin-like protein that is a constituent of the ATG8-conjugation system, one of two evolutionarily conserved phosphatidylethanolamine conjugation systems necessary for the formation of the autophagosome. The human ATG8 system includes seven ubiquitin-like light chain proteins (LCPs) that are homologs of yeast LC3: MAP1LC3A, -B, -C, GABARAP, GABARAPL1, -2, and -3. Pro-LCPs are cleaved by ATG4B to expose a C-terminal glycine residue, the cytosolic LCP-I form. The exposed C-terminus is conjugated to the head group amine of phosphatidylethanolamine (PE) through an amide bond by a sequence of ubiquitination-like reactions that involves an E1 (ATG7), an E2 (ATG3), and an E3 (a complex including ATG5, ATG12, and ATG16L). The PE-congugated form (LCP-II) is tightly associated with the autophagosomal membrane. The LCP-II forms can also be delipidated by the ATG4 proteases: most of the LCPs are delipidated and liberated from the membrane before autophagosomes fuse with lysosomes. May play a role in intracellular transport of GABA(A) receptors and its interaction with the cytoskeleton. Belongs to the MAP1 LC3 family. Interacts with GPHN and NSF. Interacts with GABRG2, beta-tubulin and ULK1.

Protein type: Vesicle; Adaptor/scaffold; Microtubule-binding; Autophagy; Ubiquitin-like modifier

Chromosomal Location of Human Ortholog: 17p13.1

Cellular Component: actin cytoskeleton; autophagic vacuole; axoneme; cytoplasmic vesicle; cytosol; Golgi membrane; lysosome; microtubule; microtubule associated complex; perinuclear region of cytoplasm; plasma membrane; smooth endoplasmic reticulum

Molecular Function: beta-tubulin binding; GABA receptor binding; microtubule binding; protein binding; ubiquitin protein ligase binding

Biological Process: induction of apoptosis via death domain receptors; macroautophagy; microtubule cytoskeleton organization and biogenesis; protein targeting; synaptic transmission
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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