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anti-GPI antibody :: Rabbit anti-Human Glucose-6-phosphate isomerase Polyclonal Antibody

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Catalog # MBS7001878
Unit / Price
  0.05 mg  /  $160 +1 FREE 8GB USB
  0.1 mg  /  $235 +1 FREE 8GB USB
Product Name

Glucose-6-phosphate isomerase (GPI), Polyclonal Antibody

Popular Item
Full Product Name

Rabbit anti-human Glucose-6-phosphate isomerase polyclonal Antibody

Product Synonym Names
Autocrine motility factor; Phosphoglucose isomerase; Phosphohexose isomerase; Sperm antigen 36
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
OMIM
172400
3D Structure
ModBase 3D Structure for P06744
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Purity/Purification
Caprylic Acid Ammonium Sulfate Precipitation purified
Form/Format
Liquid
Immunogen
Recombinant human Glucose-6-phosphate isomerase
Conjugation
Non-conjugated
Storage Buffer
Preservative: 0.03% Proclin 300; Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Santa Cruz Alternative
Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-30392 / sc-33777
Preparation and Storage
Shipped at 4 degree C. Upon delivery, aliquot and store at -20 degree C or -80 degree C.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Other Notes
Small volumes of anti-GPI antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-GPI antibody
Besides it's role as a glycolytic enzyme, mammalian GPI can function as a tumor-secreted cytokine and an angiogenic factor (AMF) that stimulates endothelial cell motility. GPI is also a neurotrophic factor (Neuroleukin) for spinal and sensory neurons.

This gene belongs to the GPI family whose members encode multifunctional phosphoglucose isomerase proteins involved in energy pathways. This protein is a dimeric enzyme that catalyzes the reversible isomerization of glucose-6-phosphate and fructose-6-phosphate. The protein functions in different capacities inside and outside the cell. In the cytoplasm, the gene product is involved in glycolysis and gluconeogenesis, while outside the cell it functions as a neurotrophic factor for spinal and sensory neurons. Defects in this gene are the cause of nonspherocytic hemolytic anemia and a severe enzyme deficiency can be associated with hydrops fetalis, immediate neonatal death and neurological impairment.
Product Categories/Family for anti-GPI antibody
Applications Tested/Suitable for anti-GPI antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes for anti-GPI antibody
Recommended dilution: IHC: 1:20-1:200

Immunohistochemistry (IHC) of anti-GPI antibody
Immunohistochemistry of paraffin-embeded human kidney using MBS7001878 at dilution of 1:100
anti-GPI antibody Immunohistochemistry (IHC) (IHC) image
Western Blot (WB) of anti-GPI antibody
Western Blot
Positive WB detected in: PC3 whole cell lysate,Hela whole cell lysate,Rat brain tissue,Mouse heart tissue,Mouse liver tissue,Mouse kidney tissue,Mouse brain tissue
All lanes: GPI antibody at 2.7ug/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 64 KDa
Observed band size: 64 KDa

anti-GPI antibody Western Blot (WB) (WB) image
Immunohistochemistry (IHC) of anti-GPI antibody
Immunohistochemistry of paraffin-embedded human colon cancer using MBS7001878 at dilution of 1:100
anti-GPI antibody Immunohistochemistry (IHC) (IHC) image
Immunohistochemistry (IHC) of anti-GPI antibody
Immunohistochemistry of paraffin-embedded human endometrial cancer using MBS7001878 at dilution of 1:100
anti-GPI antibody Immunohistochemistry (IHC) (IHC) image
Immunofluorescence (IF) of anti-GPI antibody
Immunofluorescent analysis of PC3 cells using MBS7001878 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
anti-GPI antibody Immunofluorescence (IF) image
Immunoprecipitation (IP) of anti-GPI antibody
Immunoprecipitating GPI in Hela whole cell lysate
Lane 1: Rabbit monoclonal IgG (1ug)instead of MBS7001878 in Hela whole cell lysate. For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: MBS7001878 (8ug)+ Hela whole cell lysate (500ug)
Lane 3: Hela whole cell lysate (10ug)

anti-GPI antibody Immunoprecipitation (IP) (IP) image
NCBI/Uniprot data below describe general gene information for GPI. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
64,325 Da
NCBI Official Full Name
glucose-6-phosphate isomerase isoform 2
NCBI Official Synonym Full Names
glucose-6-phosphate isomerase
NCBI Official Symbol
NCBI Official Synonym Symbols
AMF; NLK; PGI; PHI; GNPI; SA36; SA-36
  [Similar Products]
NCBI Protein Information
glucose-6-phosphate isomerase
UniProt Protein Name
Glucose-6-phosphate isomerase
UniProt Synonym Protein Names
Autocrine motility factor; AMF; Neuroleukin; NLK; Phosphoglucose isomerase; PGI; Phosphohexose isomerase; PHI; Sperm antigen 36; SA-36
UniProt Gene Name
UniProt Synonym Gene Names
GPI; AMF; NLK; PGI; PHI; SA-36  [Similar Products]
UniProt Entry Name
G6PI_HUMAN
NCBI Summary for GPI
This gene encodes a member of the glucose phosphate isomerase protein family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. In the cytoplasm, the gene product functions as a glycolytic enzyme (glucose-6-phosphate isomerase) that interconverts glucose-6-phophsate and fructose-6-phosphate. Extracellularly, the encoded protein (also referred to as neuroleukin) functions as a neurotrophic factor that promotes survival of skeletal motor neurons and sensory neurons, and as a lymphokine that induces immunoglobulin secretion. The encoded protein is also referred to as autocrine motility factor based on an additional function as a tumor-secreted cytokine and angiogenic factor. Defects in this gene are the cause of nonspherocytic hemolytic anemia and a severe enzyme deficiency can be associated with hydrops fetalis, immediate neonatal death and neurological impairment. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]
UniProt Comments for GPI
G6PI: belongs to the GPI family whose members encode multifunctional phosphoglucose isomerase proteins involved in energy pathways. A dimeric enzyme that catalyzes the reversible isomerization of glucose-6-phosphate and fructose-6-phosphate. Functions in different capacities inside and outside the cell. In the cytoplasm, the gene product is involved in glycolysis and gluconeogenesis, while outside the cell it functions as a neurotrophic factor for spinal and sensory neurons. Defects in this gene are the cause of nonspherocytic hemolytic anemia and a severe enzyme deficiency can be associated with hydrops fetalis, immediate neonatal death and neurological impairment.

Protein type: Carbohydrate Metabolism - glycolysis and gluconeogenesis; Cytokine; Carbohydrate Metabolism - starch and sucrose; Apoptosis; Carbohydrate Metabolism - amino sugar and nucleotide sugar; Carbohydrate Metabolism - pentose phosphate pathway; Isomerase; EC 5.3.1.9

Chromosomal Location of Human Ortholog: 19q13.1

Cellular Component: cytoplasm; cytosol; extracellular space; membrane; neuron projection; nucleoplasm; plasma membrane

Molecular Function: cytokine activity; glucose-6-phosphate isomerase activity; growth factor activity; intramolecular transferase activity; monosaccharide binding; ubiquitin protein ligase binding

Biological Process: aldehyde catabolic process; angiogenesis; carbohydrate metabolic process; gene expression; gluconeogenesis; glucose 6-phosphate metabolic process; glucose metabolic process; hemostasis; humoral immune response; learning and/or memory; methylglyoxal biosynthetic process; negative regulation of caspase activity; negative regulation of neuron apoptosis; transcription initiation from RNA polymerase II promoter

Disease: Hemolytic Anemia, Nonspherocytic, Due To Glucose Phosphate Isomerase Deficiency
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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