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anti-GCPII antibody :: Rabbit anti-Human Glutamate Carboxypeptidase II Polyclonal Antibody

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Catalog # MBS2028247 (SPECIAL PROMOTION: Get FREE $5 Starbucks gift card (Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Unit / Price
Scan QR to view Datasheet
  0.01 mg  /  $110 +1 FREE 8GB USB
  0.02 mg  /  $130 +1 FREE 8GB USB
  0.05 mg  /  $175 +1 FREE 8GB USB
  0.1 mg  /  $225 +1 FREE 8GB USB
  0.2 mg  /  $340 +1 FREE 8GB USB
  1 mg  /  $800 +1 FREE 8GB USB
Product Name

Glutamate Carboxypeptidase II (GCPII), Polyclonal Antibody

Popular Item
Full Product Name

Polyclonal Antibody to Glutamate Carboxypeptidase II (GCPII)

Matching Pairs
Matching Pairs
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
OMIM
M99487 mRNA
3D Structure
ModBase 3D Structure for Q04609
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Specificity
The antibody is a rabbit polyclonal antibody raised against GCPII. It has beenselected for its ability to recognize GCPII in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Liquid
Concentration
200ug/ml (lot specific)
Fragment
GCPII (Ala274~Leu587)
Organism Species
Homo sapiens (Human)
Immunogen
Recombinant GCPII (Ala274~Leu587) with two N-terminal Tags, His-tag and T7-tag expressed in E.coli.
Accession No.
RPC512Hu01
UOM
100ug
Conjugate
No Conjugate
Immunogen
Recombinant GCPII (Ala274~Leu587) expressed in E Coli.
Form & Buffer
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2066319
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
ISO Certification
Manufactured in an ISO 9001:2008 and ISO 13485:2003 Certified Laboratory.
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.
Other Notes
Small volumes of anti-GCPII antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-GCPII antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA)
Application Notes for anti-GCPII antibody
Western blotting: 1:50-400
Immunocytochemistry in formalin fixed cells: 1:50-500
Immunohistochemistry in formalin fixed frozen section: 1:50-500
Immunohistochemistry in paraffin section: 1:10-100
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user.

Western Blot (WB) of anti-GCPII antibody
Western Blot: Sample: Rat Prostate Gland Tissue.
anti-GCPII antibody Western Blot (WB) (WB) image
Western Blot (WB) of anti-GCPII antibody
Western Blot: Sample: Recombinant GCPII, Human.
anti-GCPII antibody Western Blot (WB) (WB) image
Immunohistochemistry (IHC) of anti-GCPII antibody
DAB staining on IHC-P; Samples: Human Prostate Gland Tissue.
anti-GCPII antibody Immunohistochemistry (IHC) (IHC) image
Immunohistochemistry (IHC) of anti-GCPII antibody
DAB staining on IHC-P; Samples: Human Kidney Tissue
anti-GCPII antibody Immunohistochemistry (IHC) (IHC) image
Immunohistochemistry (IHC) of anti-GCPII antibody
DAB staining on IHC-P; Samples: Human Prostate Gland Cancer Tissue.
anti-GCPII antibody Immunohistochemistry (IHC) (IHC) image
NCBI/Uniprot data below describe general gene information for GCPII. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
50,090 Da
NCBI Official Full Name
glutamate carboxypeptidase 2 isoform 2
NCBI Official Synonym Full Names
folate hydrolase 1
NCBI Official Symbol
FOLH1  [Similar Products]
NCBI Official Synonym Symbols
PSM; FGCP; FOLH; GCP2; PSMA; mGCP; GCPII; NAALAD1; NAALAdase
  [Similar Products]
NCBI Protein Information
glutamate carboxypeptidase 2
UniProt Protein Name
Glutamate carboxypeptidase 2
UniProt Synonym Protein Names
Cell growth-inhibiting gene 27 protein; Folate hydrolase 1; Folylpoly-gamma-glutamate carboxypeptidase; FGCP; Glutamate carboxypeptidase II; GCPII; Membrane glutamate carboxypeptidase; mGCP; N-acetylated-alpha-linked acidic dipeptidase I; NAALADase I; Prostate-specific membrane antigen; PSM; PSMA; Pteroylpoly-gamma-glutamate carboxypeptidase
UniProt Gene Name
FOLH1  [Similar Products]
UniProt Synonym Gene Names
FOLH; NAALAD1; PSM; PSMA; FGCP; GCPII; mGCP; NAALADase I; PSM; PSMA  [Similar Products]
NCBI Summary for GCPII
This gene encodes a type II transmembrane glycoprotein belonging to the M28 peptidase family. The protein acts as a glutamate carboxypeptidase on different alternative substrates, including the nutrient folate and the neuropeptide N-acetyl-l-aspartyl-l-glutamate and is expressed in a number of tissues such as prostate, central and peripheral nervous system and kidney. A mutation in this gene may be associated with impaired intestinal absorption of dietary folates, resulting in low blood folate levels and consequent hyperhomocysteinemia. Expression of this protein in the brain may be involved in a number of pathological conditions associated with glutamate excitotoxicity. In the prostate the protein is up-regulated in cancerous cells and is used as an effective diagnostic and prognostic indicator of prostate cancer. This gene likely arose from a duplication event of a nearby chromosomal region. Alternative splicing gives rise to multiple transcript variants encoding several different isoforms. [provided by RefSeq, Jul 2010]
UniProt Comments for GCPII
FOLH1: Has both folate hydrolase and N-acetylated-alpha-linked- acidic dipeptidase (NAALADase) activity. Has a preference for tri- alpha-glutamate peptides. In the intestine, required for the uptake of folate. In the brain, modulates excitatory neurotransmission through the hydrolysis of the neuropeptide, N- aceylaspartylglutamate (NAAG), thereby releasing glutamate. Isoform PSM-4 and isoform PSM-5 would appear to be physiologically irrelevant. Involved in prostate tumor progression. Belongs to the peptidase M28 family. M28B subfamily. 8 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.4.17.21; Membrane protein, integral; Protease

Chromosomal Location of Human Ortholog: 11p11.12

Cellular Component: cell surface; cytoplasm; integral component of plasma membrane; membrane; plasma membrane

Molecular Function: dipeptidase activity; metal ion binding; metallocarboxypeptidase activity; peptidase activity

Biological Process: amino acid biosynthetic process; folic acid-containing compound metabolic process; proteolysis
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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