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anti-GRN antibody :: Rabbit anti-Human Granulin (GRN) Polyclonal Antibody

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Catalog # MBS2015017 (SPECIAL PROMOTION: Get FREE $5 Starbucks gift card (Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Unit / Price
  0.01 mg  /  $110 +1 FREE 8GB USB
  0.02 mg  /  $130 +1 FREE 8GB USB
  0.05 mg  /  $175 +1 FREE 8GB USB
  0.1 mg  /  $225 +1 FREE 8GB USB
  0.2 mg  /  $340 +1 FREE 8GB USB
  1 mg  /  $800 +1 FREE 8GB USB
Product Name

Granulin (GRN), Polyclonal Antibody

Popular Item
Also Known As

Polyclonal Antibody to Granulin (GRN)

Product Gene Name
Matching Pairs
Matching Pairs
Unconjugated Antibody: Granulin (GRN) (MBS2015017)
APC-CY7 Conjugated Antibody: Granulin (GRN) (MBS2066337)
Matching Pairs
Unconjugated Antibody: Granulin (GRN) (MBS2015017)
PE Conjugated Antibody: Granulin (GRN) (MBS2066340)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
593
Immunogen Sequence
Antigen: The target protein is fused with two N-terminal Tags, His-tag and S-tag and its sequence is listed below.
MHHHHHHSSG LVPRGSGMKE TAAAKFERQH MDSPDLGTDD DDKAMADIGS EF- KRDVPCDNVS SCPSSDTCCQ LTSGEWGCCP IPEAVCCSDH QHCCPQGYTC VAEGQCQRGS EIVAGLEKMP ARRASLSHPR DIGCDQHTSC PVGQTCCPSL GGSWACCQLP HAVCCEDRQH CCPAGYTCNV KARSCEKEVV SAQPATFLAR SPHVGVKDVE CGEGHFCHDN QTCCRDNRQG WACCPYRQGV CCADRRHCCP
OMIM
X62320 mRNA
3D Structure
ModBase 3D Structure for P28799
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Specificity
The antibody is a rabbit polyclonal antibody raised against GRN. It has been selected for its ability to recognize GRN in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
Concentration
200ug/ml (lot specific)
Immunogen
Recombinant GRN (Lys361~Arg585) expressed in E.coli.
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2066343
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Other Notes
Small volumes of anti-GRN antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Product Categories/Family for anti-GRN antibody
Applications Tested/Suitable for anti-GRN antibody
Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
Application Notes for anti-GRN antibody
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200

Western Blot (WB) of anti-GRN antibody
Western Blot: Sample: Human A431 cell lysate; Primary Ab: 1ug/ml Rabbit Anti-Human GRN Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
anti-GRN antibody Western Blot (WB) (WB) image
Western Blot (WB) of anti-GRN antibody
Western Blot: Sample: Recombinant protein.
anti-GRN antibody Western Blot (WB) (WB) image
Immunohistochemistry (IHC) of anti-GRN antibody
DAB staining on fromalin fixed paraffin-embedded Kidney tissue)
anti-GRN antibody Immunohistochemistry (IHC) (IHC) image
Knockout Validation of anti-GRN antibody
Knockout Validation: Lane 1: Wild-type A431 cell lysate; Lane 2: GRN knockout A431 cell lysate; Predicted MW: 44,47,64kd Observed MW: 64kd Primary Ab: 1ug/ml Rabbit Anti-Human GRN Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (#MBS2086047)
anti-GRN antibody Knockout Validation image
NCBI/Uniprot data below describe general gene information for GRN. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
44,132 Da
NCBI Official Full Name
granulins
NCBI Official Synonym Full Names
granulin precursor
NCBI Official Symbol
NCBI Official Synonym Symbols
GEP; GP88; PEPI; PGRN; CLN11; PCDGF
  [Similar Products]
NCBI Protein Information
granulins
UniProt Protein Name
Granulins
UniProt Synonym Protein Names
Proepithelin; PEPI
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
PEPI; GP88; Glycoprotein 88  [Similar Products]
NCBI Summary for GRN
Granulins are a family of secreted, glycosylated peptides that are cleaved from a single precursor protein with 7.5 repeats of a highly conserved 12-cysteine granulin/epithelin motif. The 88 kDa precursor protein, progranulin, is also called proepithelin and PC cell-derived growth factor. Cleavage of the signal peptide produces mature granulin which can be further cleaved into a variety of active, 6 kDa peptides. These smaller cleavage products are named granulin A, granulin B, granulin C, etc. Epithelins 1 and 2 are synonymous with granulins A and B, respectively. Both the peptides and intact granulin protein regulate cell growth. However, different members of the granulin protein family may act as inhibitors, stimulators, or have dual actions on cell growth. Granulin family members are important in normal development, wound healing, and tumorigenesis. [provided by RefSeq, Jul 2008]
UniProt Comments for GRN
GRN: Granulins have possible cytokine-like activity. They may play a role in inflammation, wound repair, and tissue remodeling. Defects in GRN are the cause of ubiquitin-positive frontotemporal dementia (UP-FTD); also known as tau- negative frontotemporal dementia linked to chromosome 17. Frontotemporal dementia (FTD) is the second most common cause of dementia in people under the age of 65 years. It is an autosomal dominant neurodegenerative disease. Defects in GRN are the cause of neuronal ceroid lipofuscinosis type 11 (CLN11). A form of neuronal ceroid lipofuscinosis characterized by rapidly progressive visual loss due to retinal dystrophy, seizures, cerebellar ataxia, and cerebellar atrophy. Cognitive decline may also occur. Neuronal ceroid lipofuscinoses are progressive neurodegenerative, lysosomal storage diseases characterized by intracellular accumulation of autofluorescent liposomal material. Belongs to the granulin family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 17q21.31

Cellular Component: endoplasmic reticulum; endosome; extracellular region; lysosome

Molecular Function: cytokine activity; growth factor activity; protein binding; RNA binding

Biological Process: neutrophil degranulation; signal transduction

Disease: Ceroid Lipofuscinosis, Neuronal, 11; Frontotemporal Lobar Degeneration With Tdp43 Inclusions, Grn-related
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Pathways associated with anti-GRN antibody
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