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anti-HCK antibody :: Rabbit anti-Human HCK Polyclonal Antibody

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Catalog # MBS9211135
Unit / Price
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  0.08 mL  /  $165 +1 FREE 8GB USB
  0.4 mL  /  $370 +1 FREE 8GB USB
Western Blot (WB)
Product Name

HCK, Polyclonal Antibody

Full Product Name

HCK Antibody (N-term)

Product Synonym Names
Tyrosine-protein kinase HCK; Hematopoietic cell kinase; Hemopoietic cell kinase; p59-HCK/p60-HCK; p59Hck; p61Hck; HCK
Product Gene Name
Antibody/Peptide Pairs
HCK peptide (MBS9227188) is used for blocking the activity of HCK antibody (MBS9211135)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Positions
1-30
OMIM
142370
3D Structure
ModBase 3D Structure for P08631
Clonality
Polyclonal
Isotype
Rabbit Ig
Host
Rabbit
Species Reactivity
Human
Specificity
This HCK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human HCK.
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.45 (lot specific)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Other Notes
Small volumes of anti-HCK antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-HCK antibody
HCK is a protein-tyrosine kinase that is predominantly expressed in hemopoietic cell types. The encoded protein may help couple the Fc receptor to the activation of the respiratory burst. In addition, it may play a role in neutrophil migration and in the degranulation of neutrophils. Alternate translation initiation site usage, including a non-AUG (CUG) codon, results in the production of two different isoforms, that have different subcellular localization.
Product Categories/Family for anti-HCK antibody
Applications Tested/Suitable for anti-HCK antibody
Western Blot (WB), ELISA (EIA)
Application Notes for anti-HCK antibody
WB~~1:1000

Western Blot (WB) of anti-HCK antibody
HCK Antibody (E8) western blot analysis in human placenta tissue lysates (35ug/lane).This demonstrates the HCK antibody detected the HCK protein (arrow).
anti-HCK antibody Western Blot (WB) (WB) image
NCBI/Uniprot data below describe general gene information for HCK. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
59600
NCBI Official Full Name
tyrosine-protein kinase HCK isoform b
NCBI Official Synonym Full Names
HCK proto-oncogene, Src family tyrosine kinase
NCBI Official Symbol
NCBI Official Synonym Symbols
JTK9; p59Hck; p61Hck
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NCBI Protein Information
tyrosine-protein kinase HCK
UniProt Protein Name
Tyrosine-protein kinase HCK
UniProt Synonym Protein Names
Hematopoietic cell kinase; Hemopoietic cell kinase; p59-HCK/p60-HCK; p59Hck; p61Hck
Protein Family
UniProt Gene Name
UniProt Entry Name
HCK_HUMAN
NCBI Summary for HCK
The protein encoded by this gene is a member of the Src family of tyrosine kinases. This protein is primarily hemopoietic, particularly in cells of the myeloid and B-lymphoid lineages. It may help couple the Fc receptor to the activation of the respiratory burst. In addition, it may play a role in neutrophil migration and in the degranulation of neutrophils. Multiple isoforms with different subcellular distributions are produced due to both alternative splicing and the use of alternative translation initiation codons, including a non-AUG (CUG) codon. [provided by RefSeq, Feb 2010]
UniProt Comments for HCK
Hck: a protein-tyrosine kinase of the Src family that is predominantly expressed in hemopoietic cell types. The encoded protein may help couple the Fc receptor to the activation of the respiratory burst. In addition, it may play a role in neutrophil migration and in the degranulation of neutrophils. Alternate translation initiation site usage, including a non-AUG (CUG) codon, results in the production of two different isoforms that have different subcellular localization.

Protein type: Protein kinase, tyrosine (non-receptor); Protein kinase, TK; Kinase, protein; Oncoprotein; EC 2.7.10.2; TK group; Src family

Chromosomal Location of Human Ortholog: 20q11-q12

Cellular Component: Golgi apparatus; transport vesicle; extrinsic to internal side of plasma membrane; focal adhesion; cell projection; lysosome; actin filament; caveola; cytosol; nucleus

Molecular Function: protein binding; protein-tyrosine kinase activity; non-membrane spanning protein tyrosine kinase activity; ATP binding; receptor binding

Biological Process: respiratory burst after phagocytosis; peptidyl-tyrosine phosphorylation; viral reproduction; protein amino acid autophosphorylation; regulation of phagocytosis; protein amino acid phosphorylation; regulation of cell shape; leukocyte migration during immune response; regulation of transcription factor activity; leukocyte degranulation; positive regulation of cell proliferation; lipopolysaccharide-mediated signaling pathway; mesoderm development; cell differentiation; innate immune response-activating signal transduction; inflammatory response; cell adhesion; integrin-mediated signaling pathway; cytokine and chemokine mediated signaling pathway; regulation of defense response to virus by virus; positive regulation of actin filament polymerization; regulation of inflammatory response; innate immune response; transmembrane receptor protein tyrosine kinase signaling pathway; negative regulation of apoptosis
Product References and Citations for anti-HCK antibody
Komuro, I., et al., J. Exp. Med. 198(3):443-453 (2003).
Lake, J.A., et al., J. Clin. Virol. 26(2):143-152 (2003).
Stanglmaier, M., et al., Leukemia 17(2):283-289 (2003).
Lerner, E.C., et al., Nat. Struct. Biol. 9(5):365-369 (2002).
Chang, A.H., et al., Eur. J. Immunol. 31(8):2382-2387 (2001).

Precautions
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Disclaimer
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