• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

anti-HDAC1 antibody :: Rabbit anti-Human HDAC1 Polyclonal Antibody

Scan QR to view Datasheet
Catalog # MBS1495517
Unit / Price
Scan QR to view Datasheet
  0.05 mg  /  $160 +1 FREE 8GB USB
  0.1 mg  /  $235 +1 FREE 8GB USB
Product Name

HDAC1, Polyclonal Antibody

Popular Item
Full Product Name

HDAC1 Antibody

Product Synonym Names
Histone deacetylase 1 (EC:3.5.1.98); HD1; HDAC1; RPD3L1
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
OMIM
601241
3D Structure
ModBase 3D Structure for Q13547
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Purity/Purification
>95%, Protein G Purified
Storage Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Conjugate
Non-conjugated
Immunogen
Recombinant human Histone deacetylase 1 protein
Santa Cruz Alternative
Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-6298 / sc-6299 / sc-7872
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Other Notes
Small volumes of anti-HDAC1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-HDAC1 antibody
Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Deacetylates SP proteins, SP1 and SP3, and regulates their function. Component of the BRG1-RB1-HDAC1 complex, which negatively regulates the CREST-mediated transcription in resting neurons. Upon calcium stimulation, HDAC1 is released from the complex and CREBBP is recruited, which facilitates transcriptional activation. Deacetylates TSHZ3 and regulates its transcriptional repressor activity. Deacetylates 'Lys-310' in RELA and thereby inhibits the transcriptional activity of NF-kappa-B. Deacetylates NR1D2 and abrogates the effect of KAT5-mediated relieving of NR1D2 transcription repression activity. Component of a RCOR/GFI/KDM1A/HDAC complex that suppresses, via histone deacetylase (HDAC) recruitment, a number of genes implicated in multilineage blood cell development. Involved in CIART-mediated transcriptional repression of the circadian transcriptional activator: CLOCK-ARNTL/BMAL1 heterodimer. Required for the transcriptional repression of circadian target genes, such as PER1, mediated by the large PER complex or CRY1 through histone deacetylation.
Applications Tested/Suitable for anti-HDAC1 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Chromatin Immunoprecipitation (ChIP)

Immunohistochemistry (IHC) of anti-HDAC1 antibody
Immunohistochemistry of paraffin-embedded human testis using MBS1495517 at dilution 1:100
anti-HDAC1 antibody Immunohistochemistry (IHC) (IHC) image
Immunohistochemistry (IHC) of anti-HDAC1 antibody
Immunohistochemistry of paraffin-embedded human colon cancer using MBS1495517 at dilution 1:100
anti-HDAC1 antibody Immunohistochemistry (IHC) (IHC) image
Immunohistochemistry (IHC) of anti-HDAC1 antibody
Immunohistochemistry of paraffin-embedded human melanoma cancer using MBS1495517 at dilution of 1:100
anti-HDAC1 antibody Immunohistochemistry (IHC) (IHC) image
Immunohistochemistry (IHC) of anti-HDAC1 antibody
Immunohistochemistry of paraffin-embedded human thyroid tissue using MBS1495517 at dilution of 1:100
anti-HDAC1 antibody Immunohistochemistry (IHC) (IHC) image
Immunofluorescence (IF) of anti-HDAC1 antibody
Immunofluorescence staining of Hela cells with MBS1495517 at 1:133,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
anti-HDAC1 antibody Immunofluorescence (IF) image
Chromatin Immunoprecipitation (ChIP) of anti-HDAC1 antibody
Chromatin Immunoprecipitation MCF7(1.1^106)were cross-linked with formaldehyde, sonicated, and immunoprecipitated with 4ug anti-HDAC1 or a control normal rabbit IgG . The resulting ChIP DNA was quantified using real-time PCR with primers against the P21 promoter.
anti-HDAC1 antibody Chromatin Immunoprecipitation (ChIP) (IP) image
NCBI/Uniprot data below describe general gene information for HDAC1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
55,103 Da
NCBI Official Full Name
histone deacetylase 1
NCBI Official Synonym Full Names
histone deacetylase 1
NCBI Official Symbol
HDAC1  [Similar Products]
NCBI Official Synonym Symbols
HD1; RPD3; GON-10; RPD3L1
  [Similar Products]
NCBI Protein Information
histone deacetylase 1
UniProt Protein Name
Histone deacetylase 1
UniProt Gene Name
HDAC1  [Similar Products]
UniProt Synonym Gene Names
RPD3L1; HD1  [Similar Products]
UniProt Entry Name
HDAC1_HUMAN
NCBI Summary for HDAC1
Histone acetylation and deacetylation, catalyzed by multisubunit complexes, play a key role in the regulation of eukaryotic gene expression. The protein encoded by this gene belongs to the histone deacetylase/acuc/apha family and is a component of the histone deacetylase complex. It also interacts with retinoblastoma tumor-suppressor protein and this complex is a key element in the control of cell proliferation and differentiation. Together with metastasis-associated protein-2, it deacetylates p53 and modulates its effect on cell growth and apoptosis. [provided by RefSeq, Jul 2008]
UniProt Comments for HDAC1
HDAC1: a transcriptional regulator of the histone deacetylase family, subfamily 1. Deacetylates lysine residues on the N-terminal part of the core histones H2A, H2B, H3 AND H4. Plays an important role in transcriptional regulation, cell cycle progression and developmental events.

Protein type: EC 3.5.1.98; Nuclear receptor co-regulator; Hydrolase

Chromosomal Location of Human Ortholog: 1p34

Cellular Component: chromatin; cytoplasm; cytosol; histone deacetylase complex; nuclear chromatin; nucleoplasm; nucleus; NuRD complex; protein complex; Sin3 complex

Molecular Function: deacetylase activity; enzyme binding; histone deacetylase activity; histone deacetylase binding; NAD-dependent histone deacetylase activity (H3-K14 specific); NF-kappaB binding; nucleosomal DNA binding; protein binding; protein deacetylase activity; protein N-terminus binding; transcription activator binding; transcription factor activity; transcription factor binding

Biological Process: ATP-dependent chromatin remodeling; blood coagulation; chromatin modification; chromatin remodeling; circadian regulation of gene expression; embryonic digit morphogenesis; epidermal cell differentiation; establishment and/or maintenance of chromatin architecture; gene expression; histone deacetylation; mitotic cell cycle; negative regulation of apoptosis; negative regulation of cell cycle; negative regulation of gene expression, epigenetic; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; nerve growth factor receptor signaling pathway; Notch signaling pathway; odontogenesis of dentine-containing teeth; positive regulation of cell proliferation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; protein amino acid deacetylation; regulation of gene expression, epigenetic; transcription initiation from RNA polymerase II promoter; transcription, DNA-dependent; transforming growth factor beta receptor signaling pathway; viral reproduction
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000