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anti-HK2 antibody :: Rabbit anti-Human HK2 Polyclonal Antibody

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Catalog # MBS7052049
Unit / Price
  0.05 mg  /  $160 +1 FREE 8GB USB
  0.1 mg  /  $235 +1 FREE 8GB USB
Product Name

HK2, Polyclonal Antibody

Popular Item
Also Known As

HK2 Antibody

Product Synonym Names
Hexokinase-2; Hexokinase type II; HK II; Muscle form hexokinase; HK2
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
917
OMIM
Z46376 mRNA
3D Structure
ModBase 3D Structure for P52789
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Purity/Purification
>95%, Protein G purified
Form/Format
Liquid
Immunogen
Recombinant Human Hexokinase-2 protein (334-526AA)
Conjugation
Non-conjugated
Preservative
0.03% Proclin 300
Constituents
50% Glycerol, 0.01M PBS, pH 7.4
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Other Notes
Small volumes of anti-HK2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-HK2 antibody
cytosol, membrane, mitochondrial outer membrane, fructokinase activity, glucokinase activity, hexokinase activity, mannokinase activity, apoptotic mitochondrial changes, canonical glycolysis, cellular glucose homeostasis
Applications Tested/Suitable for anti-HK2 antibody
ELISA (EIA), Western Blot (WB), Immunofluorescence (IF)
Application Notes for anti-HK2 antibody
WB: 1:500-1:5000
IF: 1:50-1:200

Western Blot (WB) of anti-HK2 antibody
Western Blot
Positive WB detected in: U87 whole cell lysate
All lanes: HK2 antibody at 4ug/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
predicted band size: 103 kDa
observed band size: 103 kDa

anti-HK2 antibody Western Blot (WB) (WB) image
Immunofluorescence (IF) of anti-HK2 antibody
Immunofluorescent analysis of PC3 cells using MBS7052049 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
anti-HK2 antibody Immunofluorescence (IF) image
NCBI/Uniprot data below describe general gene information for HK2. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
102,380 Da
NCBI Official Full Name
hexokinase-2
NCBI Official Synonym Full Names
hexokinase 2
NCBI Official Symbol
NCBI Official Synonym Symbols
HKII; HXK2
  [Similar Products]
NCBI Protein Information
hexokinase-2
UniProt Protein Name
Hexokinase-2
UniProt Synonym Protein Names
Hexokinase type II; HK II; Muscle form hexokinase
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
HK II  [Similar Products]
NCBI Summary for HK2
Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. This gene encodes hexokinase 2, the predominant form found in skeletal muscle. It localizes to the outer membrane of mitochondria. Expression of this gene is insulin-responsive, and studies in rat suggest that it is involved in the increased rate of glycolysis seen in rapidly growing cancer cells. [provided by RefSeq, Apr 2009]
UniProt Comments for HK2
HK2: a glycolytic enzyme that catalyzes the reaction ATP + D-hexose = ADP + D-hexose 6-phosphate. The first and rate-limiting step in glycosis, a pathway that produces energy in the form of ATP from glucose. An allosteric enzyme inhibited by its product glucose-6-phosphate (Glc-6-P). The predominant hexokinase isozyme expressed in insulin-responsive tissues such as skeletal muscle. Acts as a ""glucose sensor"" by trapping glucose inside the cell by catalyzing its phosphorylation to produce Glc-6-P. In vertebrates there are four major glucose-phosphorylating isoenzymes, designated hexokinase I, II, III and IV (glucokinase). Genetic variations of HK2 do not appear to contribute to NIDDM.

Protein type: Carbohydrate Metabolism - amino sugar and nucleotide sugar; Carbohydrate Metabolism - fructose and mannose; Carbohydrate Metabolism - galactose; Carbohydrate Metabolism - glycolysis and gluconeogenesis; Carbohydrate Metabolism - starch and sucrose; EC 2.7.1.1; Kinase, other; Mitochondrial

Chromosomal Location of Human Ortholog: 2p12

Cellular Component: cytosol; membrane; mitochondrial outer membrane

Molecular Function: fructokinase activity; glucokinase activity; hexokinase activity; mannokinase activity; protein binding

Biological Process: apoptotic mitochondrial changes; cell glucose homeostasis; glucose transport; glycolysis
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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