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anti-IDS antibody :: Goat IDS Polyclonal Antibody

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Catalog # MBS421979
Unit / Price
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  0.1 mg  /  $300 +1 FREE 8GB USB
Product Name

IDS, Polyclonal Antibody

Full Product Name

Goat anti-IDS Antibody

Product Synonym Names
IDS; iduronate 2-sulfatase (Hunter syndrome); MPS2; SIDS; iduronate-2-sulfatase; IDS antibody; iduronate 2-sulfatase (Hunter syndrome) antibody; MPS2 antibody; SIDS antibody; iduronate-2-sulfatase antibody
Product Gene Name
Antibody/Peptide Pairs
IDS peptide (MBS426724) is used for blocking the activity of IDS antibody (MBS421979)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence
KHFRFRDLEE DP
OMIM
300823
Clonality
Polyclonal
Host
Goat
Species Reactivity
Tested: Human; Expected from sequence similarity: Human
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (lot specific)
Immunogen
Peptide with sequence C-KHFRFRDLEEDP, from the internal region of the protein sequence according to NP_000193.1.
Epitope
Internal region
Note
This antibody is expected to recognize isoform a (NP_000193.1).
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Other Notes
Small volumes of anti-IDS antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-IDS antibody
Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-IDS antibody
Peptide ELISA: Antibody detection limit dilution 1: 16000.
Immunohistochemistry: In paraffin embedded Human Placenta shows lysosomal staining of trophoblasts. Recommended concentration, 3-5ug/ml.
Western Blot: Approx. 60kDa band observed in Human Liver lysates (calculated MW of 61.9kDa according to NP_000193.1). Recommended concentration: 0.1-0.3ug/ml.

Western Blot (WB) of anti-IDS antibody
(0.1ug/ml) staining of Human Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
anti-IDS antibody Western Blot (WB) (WB) image
Immunohistochemistry (IHC) of anti-IDS antibody
(3.8ug/ml) staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
anti-IDS antibody Immunohistochemistry (IHC) (IHC) image
NCBI/Uniprot data below describe general gene information for IDS. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
34,893 Da
NCBI Official Full Name
iduronate 2-sulfatase isoform a preproprotein
NCBI Official Synonym Full Names
iduronate 2-sulfatase
NCBI Official Symbol
NCBI Official Synonym Symbols
MPS2; SIDS
  [Similar Products]
NCBI Protein Information
iduronate 2-sulfatase
UniProt Protein Name
Iduronate 2-sulfatase
UniProt Synonym Protein Names
Alpha-L-iduronate sulfate sulfatase; Idursulfase
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
SIDS; Idursulfase  [Similar Products]
UniProt Entry Name
IDS_HUMAN
NCBI Summary for IDS
This gene encodes a member of the sulfatase family of proteins. The encoded preproprotein is proteolytically processed to generate two polypeptide chains. This enzyme is involved in the lysosomal degradation of heparan sulfate and dermatan sulfate. Mutations in this gene are associated with the X-linked lysosomal storage disease mucopolysaccharidosis type II, also known as Hunter syndrome. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed. [provided by RefSeq, Jan 2016]
UniProt Comments for IDS
IDS: Required for the lysosomal degradation of heparan sulfate and dermatan sulfate. Defects in IDS are the cause of mucopolysaccharidosis type 2 (MPS2); also known as Hunter syndrome. MPS2 is an X-linked lysosomal storage disease characterized by intracellular accumulation of heparan sulfate and dermatan sulfate and their excretion in urine. Most children with MPS2 have a severe form with early somatic abnormalities including skeletal deformities, hepatosplenomegaly, and progressive cardiopulmonary deterioration. A prominent feature is neurological damage that presents as developmental delay and hyperactivity but progresses to mental retardation and dementia. They die before 15 years of age, usually as a result of obstructive airway disease or cardiac failure. In contrast, those with a mild form of MPS2 may survive into adulthood, with attenuated somatic complications and often without mental retardation. Belongs to the sulfatase family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.1.6.13; Hydrolase; Glycan Metabolism - glycosaminoglycan degradation

Chromosomal Location of Human Ortholog: Xq28

Cellular Component: lysosomal lumen

Molecular Function: iduronate-2-sulfatase activity; metal ion binding

Biological Process: chondroitin sulfate catabolic process; glycosaminoglycan catabolic process

Disease: Mucopolysaccharidosis, Type Ii
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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