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anti-IL18 antibody :: Rabbit anti-Human IL18 Polyclonal Antibody

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Catalog # MBS176767
Unit / Price
  0.1 mg  /  $315 +1 FREE 8GB USB
Product Name

IL18, Polyclonal Antibody

Popular Item
Also Known As

Anti-IL18 antibody

Product Synonym Names
Interleukin-18; interleukin 18 (interferon-gamma-inducing factor); Iboctadekin antibody; IFN gamma inducing factor antibody; IFN-gamma-inducing factor antibody; IGIF antibody; IL 1 gamma antibody; IL 18 antibody; IL 1g antibody; IL-1 gamma antibody; IL-18 antibody; IL1 gamma antibody; IL18 antibody; IL18 protein antibody; IL1F4 antibody; IL1g antibody; IL1gamma antibody; ILIF4 antibody; Interferon gamma inducing factor antibody; Interferon gamma-inducing factor antibody; Interleukin 1 gamma antibody; Interleukin 18 antibody; Interleukin-1 gamma antibody; Interleukin-18 antibody; Interleukin18 antibody; MGC12320 antibody
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
193
OMIM
600953
3D Structure
ModBase 3D Structure for Q14116
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Specificity
No cross reactivity with other proteins.
Purity/Purification
Immunogen affinity purified.
Form/Format
Lyophilized
Immunogen
E Coli-derived human IL18 recombinant protein (Position: Y37-D193).
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Preparation and Storage
At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Other Notes
Small volumes of anti-IL18 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-IL18 antibody
Description: Rabbit IgG polyclonal antibody for Interleukin-18(IL18) detection. Tested with WB, IHC-P in Human.
Background: Interleukin-18 also known as IL18 is a protein which in humans is encoded by the IL18 gene. The protein encoded by this gene is a proinflammatory cytokine.IL-18 is a cytokine produced by macrophages and other cells that belongs to the IL-1 superfamily. IL-18 works by binding to the interleukin-18 receptor, and together with IL-12 it induces cell-mediated immunity following infection with microbial products like lipopolysaccharide (LPS). After stimulation with IL-18, natural killer (NK) cells and certain T cells release another important cytokine called interferon-gamma (IFN-gamma) or type II interferon that plays an important role in activating the macrophages or other cells.The combination of this cytokine and IL12 has been shown to inhibit IL4 dependent IgE and IgG1 production, and enhance IgG2a production in B cells. IL-18 binding protein (IL18BP) can specifically interact with this cytokine, and thus negatively regulate its biological activity. The human interleukin 18 gene IL18 maps to 11q22.2-q22.3, closely linked to the DRD2 gene locus and distinct from mapped IDDM loci.
Applications Tested/Suitable for anti-IL18 antibody
Western Blot (WB), Immunohistochemistry (IHC) Paraffin
Application Notes for anti-IL18 antibody
Western Blot:
Concentration: 0.1-0.5ug/ml
Tested Species: Hu

Immunohistochemistry (Paraffin-embedded Section):
Concentration: 0.5-1ug/ml
Tested Species: Hu
Antigen Retrieval: By Heat
Immunocytochemistry:
Concentration: 0.58-1ug/ml
Tested Species: Hu

WB: The detection limit for IL18 is approximately 0.25ng/lane under reducing conditions.
By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Other applications have not been tested.
Optimal dilutions shoudl be determined by end users.

Immunohistochemistry (IHC) of anti-IL18 antibody
Anti-IL-18 Picoband antibody, MBS176767-1.JPG
IHC(P): Human Tonsil Tissue
anti-IL18 antibody Immunohistochemistry (IHC) (IHC) image
Western Blot (WB) of anti-IL18 antibody
Anti-IL-18 Picoband antibody, MBS176767-2.jpg
All lanes: Anti-IL18 (MBS176767) at 0.5ug/ml
WB: HELA Whole Cell Lysate at 40ug
Predicted bind size: 22KD
Observed bind size: 22KD
anti-IL18 antibody Western Blot (WB) (WB) image
NCBI/Uniprot data below describe general gene information for IL18. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
21,896 Da
NCBI Official Full Name
Interleukin-18
NCBI Official Synonym Full Names
interleukin 18
NCBI Official Symbol
NCBI Official Synonym Symbols
IGIF; IL-18; IL-1g; IL1F4
  [Similar Products]
NCBI Protein Information
interleukin-18; IL-1 gamma; iboctadekin; interleukin-1 gamma; IFN-gamma-inducing factor; interleukin 18 (interferon-gamma-inducing factor)
UniProt Protein Name
Interleukin-18
UniProt Synonym Protein Names
Iboctadekin; Interferon gamma-inducing factor; IFN-gamma-inducing factor; Interleukin-1 gamma; IL-1 gamma
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
IGIF; IL1F4; IL-18; IFN-gamma-inducing factor; IL-1 gamma  [Similar Products]
UniProt Entry Name
IL18_HUMAN
NCBI Summary for IL18
The protein encoded by this gene is a proinflammatory cytokine that augments natural killer cell activity in spleen cells, and stimulates interferon gamma production in T-helper type I cells. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Aug 2011]
UniProt Comments for IL18
IL18: Augments natural killer cell activity in spleen cells and stimulates interferon gamma production in T-helper type I cells. Belongs to the IL-1 family.

Protein type: Cytokine

Chromosomal Location of Human Ortholog: 11q22.2-q22.3

Cellular Component: extracellular space; extracellular region; cytosol

Molecular Function: protein binding; cytokine activity

Biological Process: positive regulation of granulocyte macrophage colony-stimulating factor production; positive regulation of interleukin-17 production; regulation of cell adhesion; T-helper 1 type immune response; positive regulation of natural killer cell proliferation; interleukin-2 biosynthetic process; T-helper 2 type immune response; positive regulation of NK T cell proliferation; MAPKKK cascade; natural killer cell activation; interleukin-13 biosynthetic process; positive regulation of NF-kappaB import into nucleus; positive regulation of activated T cell proliferation; sleep; chemokine biosynthetic process; positive regulation of tissue remodeling; positive regulation of interferon-gamma production; cell-cell signaling; lipopolysaccharide-mediated signaling pathway; interferon-gamma biosynthetic process; granulocyte macrophage colony-stimulating factor biosynthetic process; immune response; angiogenesis; inflammatory response; negative regulation of myoblast differentiation; positive regulation of inflammatory response
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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