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anti-ICOSLG antibody :: Rabbit anti-Human Inducible T-Cell Co Stimulator Ligand (ICOSLG) Polyclonal Antibody

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Catalog # MBS2014003 (SPECIAL PROMOTION: Get FREE $5 Starbucks gift card (Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Unit / Price
  0.01 mg  /  $105 +1 FREE 8GB USB
  0.02 mg  /  $125 +1 FREE 8GB USB
  0.05 mg  /  $165 +1 FREE 8GB USB
  0.1 mg  /  $210 +1 FREE 8GB USB
  0.2 mg  /  $305 +1 FREE 8GB USB
  1 mg  /  $720 +1 FREE 8GB USB
Product Name

Inducible T-Cell Co Stimulator Ligand (ICOSLG), Polyclonal Antibody

Popular Item
Also Known As

Polyclonal Antibody to Inducible T-Cell Co Stimulator Ligand (ICOSLG)

Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
199
Immunogen Sequence
Antigen: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- KEVRAMVG SDVELSCACP EGSRFDLNDV YVYWQTSESK TVVTYHIPQN SSLENVDSRY RNRALMSPAG MLRGDFSLRL FNVTPQDEQK FHCLVLSQSL GFQEVLSVEV TLHVAANFSV PVVSAPHSPS QDELTFTCTS INGYPRPNVY WINKTDNSLL DQALQNDTVF LNMRGLYDVV SVLRIARTPS VNIGCCIENV
OMIM
AF199028 mRNA
3D Structure
ModBase 3D Structure for O75144
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Specificity
The antibody is a rabbit polyclonal antibody raised against ICOSLG. It has been selected for its ability to recognize ICOSLG in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
Concentration
200ug/ml (lot specific)
Immunogen
Recombinant ICOSLG (Lys23~Thr249) expressed in E.coli.
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2047440
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Other Notes
Small volumes of anti-ICOSLG antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Product Categories/Family for anti-ICOSLG antibody
Applications Tested/Suitable for anti-ICOSLG antibody
Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
Application Notes for anti-ICOSLG antibody
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200

Western Blot (WB) of anti-ICOSLG antibody
Western Blot: Sample: Lane1: Human Serum; Lane2: Human Liver Tissue; Lane3: Human Lung Tissue; Lane4: Human Jurkat Cells.
anti-ICOSLG antibody Western Blot (WB) (WB) image
Western Blot (WB) of anti-ICOSLG antibody
Western Blot: Sample: Recombinant ICOSLG, Human.
anti-ICOSLG antibody Western Blot (WB) (WB) image
Immunohistochemistry (IHC) of anti-ICOSLG antibody
DAB staining on fromalin fixed paraffin-embedded Kidney tissue)
anti-ICOSLG antibody Immunohistochemistry (IHC) (IHC) image
Immunohistochemistry (IHC) of anti-ICOSLG antibody
DAB staining on IHC-P; Samples: Human Tonsil Tissue)
anti-ICOSLG antibody Immunohistochemistry (IHC) (IHC) image
Knockout Validation of anti-ICOSLG antibody
Knockout Validation: Lane 1: Wild-type Jurkat cell lysate; Lane 2: ICOSLG knockout Jurkat cell lysate; Predicted MW: 33kd Observed MW: 27kd Primary Ab: 2ug/ml Rabbit Anti-Human ICOSLG Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (#MBS2086047)
anti-ICOSLG antibody Knockout Validation image
NCBI/Uniprot data below describe general gene information for ICOSLG. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
20,120 Da
NCBI Official Full Name
ICOS ligand isoform b
NCBI Official Synonym Full Names
inducible T cell costimulator ligand
NCBI Official Symbol
ICOSLG  [Similar Products]
NCBI Official Synonym Symbols
B7H2; GL50; B7-H2; B7RP1; CD275; ICOSL; LICOS; B7RP-1; ICOS-L
  [Similar Products]
NCBI Protein Information
ICOS ligand
UniProt Protein Name
ICOS ligand
UniProt Synonym Protein Names
B7 homolog 2; B7-H2; B7-like protein Gl50; B7-related protein 1; B7RP-1; CD_antigen: CD275
Protein Family
UniProt Gene Name
ICOSLG  [Similar Products]
UniProt Synonym Gene Names
B7H2; B7RP1; ICOSL; KIAA0653; B7-H2; B7RP-1  [Similar Products]
UniProt Comments for ICOSLG
ICOSLG: Ligand for the T-cell-specific cell surface receptor ICOS. Acts as a costimulatory signal for T-cell proliferation and cytokine secretion; induces also B-cell proliferation and differentiation into plasma cells. Could play an important role in mediating local tissue responses to inflammatory conditions, as well as in modulating the secondary immune response by co- stimulating memory T-cell function. Belongs to the immunoglobulin superfamily. BTN/MOG family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 21q22.3

Cellular Component: integral component of membrane; plasma membrane

Molecular Function: receptor binding

Biological Process: adaptive immune response; B cell activation; defense response; hyperosmotic response; positive regulation of activated T cell proliferation; signal transduction; T cell activation; T cell costimulation
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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