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anti-IRAK4 antibody :: Rabbit anti-Human Interleukin 1 Receptor Associated Kinase 4 (IRAK4) Polyclonal Antibody

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Catalog # MBS2003511 (SPECIAL PROMOTION: Get FREE $5 Starbucks gift card (Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Unit / Price
  0.01 mg  /  $105 +1 FREE 8GB USB
  0.02 mg  /  $125 +1 FREE 8GB USB
  0.05 mg  /  $170 +1 FREE 8GB USB
  0.1 mg  /  $220 +1 FREE 8GB USB
  0.2 mg  /  $320 +1 FREE 8GB USB
  1 mg  /  $760 +1 FREE 8GB USB
Product Name

Interleukin 1 Receptor Associated Kinase 4 (IRAK4), Polyclonal Antibody

Also Known As

Polyclonal Antibody to Interleukin 1 Receptor Associated Kinase 4 (IRAK4)

Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
460
Immunogen Sequence
Antigen: The target protein is fused with N-terminal His-Tag and its sequence is listed below.
MGHHHHHHSG SEF-GN KMGEGGFGVV YKGYVNNTTV AVKKLAAMVD ITTEELKQQF DQEIKVMAKC QHENLVELLG FSSDGDDLCL VYVYMPNGSL LDRLSCLDGT PPLSWHMRCK IAQGAANGIN FLHENHHIHR DIKSANILLD EAFTAKISDF GLARASEKFA QTVMTSRIVG TTAYMAPEAL RGEITPKSDI YSFGVVLLEI ITGLPAVDEH REPQLLLDIK EEIEDEEKTI EDYIDKKMND ADSTSVE
OMIM
AF445802 mRNA
3D Structure
ModBase 3D Structure for Q9NWZ3
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Specificity
The antibody is a rabbit polyclonal antibody raised against IRAK4. It has been selected for its ability to recognize IRAK4 in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
Concentration
200ug/ml (lot specific)
Immunogen
Recombinant IRAK4 (Gly189~Glu427) expressed in E.coli.
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2056140
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Other Notes
Small volumes of anti-IRAK4 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-IRAK4 antibody
Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
Application Notes for anti-IRAK4 antibody
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200

Western Blot (WB) of anti-IRAK4 antibody
Western Blot: Sample: Lane1: Human Hela Cells; Lane2: Mouse Spleen Tissue; Lane3: Mouse Brain Tissue.
anti-IRAK4 antibody Western Blot (WB) (WB) image
Western Blot (WB) of anti-IRAK4 antibody
Western Blot: Sample: Recombinant IRAK4, Human.
anti-IRAK4 antibody Western Blot (WB) (WB) image
Immunohistochemistry (IHC) of anti-IRAK4 antibody
DAB staining on IHC-P. Samples: Human Tissue)
anti-IRAK4 antibody Immunohistochemistry (IHC) (IHC) image
NCBI/Uniprot data below describe general gene information for IRAK4. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
37,674 Da
NCBI Official Full Name
interleukin-1 receptor-associated kinase 4 isoform a
NCBI Official Synonym Full Names
interleukin 1 receptor associated kinase 4
NCBI Official Symbol
IRAK4  [Similar Products]
NCBI Official Synonym Symbols
IPD1; REN64; IRAK-4; NY-REN-64
  [Similar Products]
NCBI Protein Information
interleukin-1 receptor-associated kinase 4
UniProt Protein Name
Interleukin-1 receptor-associated kinase 4
UniProt Synonym Protein Names
Renal carcinoma antigen NY-REN-64
UniProt Gene Name
IRAK4  [Similar Products]
UniProt Synonym Gene Names
IRAK-4  [Similar Products]
NCBI Summary for IRAK4
This gene encodes a kinase that activates NF-kappaB in both the Toll-like receptor (TLR) and T-cell receptor (TCR) signaling pathways. The protein is essential for most innate immune responses. Mutations in this gene result in IRAK4 deficiency and recurrent invasive pneumococcal disease. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2011]
UniProt Comments for IRAK4
IRAK4: a TKL kinase of the IRAK family. The interleukin-1 receptor-associated kinases are important mediators in the signal transduction of Toll-like receptor and IL1R family members, collectively referred to as TIRs.

Protein type: EC 2.7.11.1; IRAK family; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); Protein kinase, TKL; TKL group

Chromosomal Location of Human Ortholog: 12q12

Cellular Component: cytoplasm; cytosol; endosome membrane; extracellular space; nucleus; plasma membrane

Molecular Function: ATP binding; interleukin-1 receptor binding; magnesium ion binding; protein binding; protein kinase activity; protein serine/threonine kinase activity

Biological Process: cytokine production; innate immune response; JNK cascade; MyD88-dependent toll-like receptor signaling pathway; neutrophil mediated immunity; neutrophil migration; positive regulation of I-kappaB kinase/NF-kappaB signaling; positive regulation of NF-kappaB transcription factor activity; positive regulation of smooth muscle cell proliferation; toll-like receptor 9 signaling pathway; toll-like receptor signaling pathway

Disease: Invasive Pneumococcal Disease, Recurrent Isolated, 1; Irak4 Deficiency
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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