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anti-ITIH4 antibody :: Goat ITIH4 Polyclonal Antibody

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Catalog # MBS421694
Unit / Price
  0.1 mg  /  $300 +1 FREE 8GB USB
Immunohistochemistry (IHC)
Product Name

ITIH4, Polyclonal Antibody

Also Known As

Goat anti-ITIH4 Antibody

Product Synonym Names
Goat Anti-ITIH4 (aa890-903) Antibody; ITIH4; inter-alpha (globulin) inhibitor H4 (plasma Kallikrein-sensitive glycoprotein); DKFZp686G21125; H4P; IHRP; ITIHL1; PK120; inter-alpha (globulin) inhibitor H4; plasma kallikrein-sensitive glycoprotein; ITIH4 antibody; inter-alpha (globulin) inhibitor H4 (plasma Kallikrein-sensitive glycoprotein) antibody; DKFZp686G21125 antibody; H4P antibody; IHRP antibody; ITIHL1 antibody; PK120 antibody; inter-alpha (globulin) inhibitor H4 antibody; plasma kallikrein-sensitive glycoprotein antibody
Antibody/Peptide Pairs
ITIH4 peptide (MBS428237) is used for blocking the activity of ITIH4 antibody (MBS421694)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
930
Immunogen Sequence
ERLCVDPRHR QGPVN
OMIM
600564
Clonality
Polyclonal
Host
Goat
Species Reactivity
Tested: Human; Expected from sequence similarity: Human
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (lot specific)
Immunogen
Peptide with sequence ERLCVDPRHRQGPVN, from the internal region of the protein sequence according to NP_002209.2.
Epitope
Internal region
Note
The immunizing peptide represents a region of the 35kDa chain. This chain is glycosylated giving rise to an apparent higher molecular weight.
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Other Notes
Small volumes of anti-ITIH4 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-ITIH4 antibody
Peptide ELISA (EIA), Immunohistochemistry (IHC)
Application Notes for anti-ITIH4 antibody
Peptide ELISA: Antibody detection limit dilution 1: 16000.
Immunohistochemistry: In paraffin embedded Human Liver shows vesicular staining in the cytoplasm of hepatocytes. Recommended concentration, 4-6ug/ml.
Western Blot: Preliminary experiments gave an approx.150kDa band in Human Liver and Lung lysates after 0. 03ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 103kDa according to NP_002209.2. The 150kDa band was successfully blocked by incubation with the immunizing peptide.

Immunohistochemistry (IHC) of anti-ITIH4 antibody
(3.8ug/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
anti-ITIH4 antibody Immunohistochemistry (IHC) (IHC) image
NCBI/Uniprot data below describe general gene information for ITIH4. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
93,852 Da
NCBI Official Full Name
inter-alpha-trypsin inhibitor heavy chain H4 isoform 1
NCBI Official Synonym Full Names
inter-alpha-trypsin inhibitor heavy chain family member 4
NCBI Official Symbol
ITIH4  [Similar Products]
NCBI Official Synonym Symbols
H4P; IHRP; GP120; PK120; ITIHL1; PK-120; ITI-HC4
  [Similar Products]
NCBI Protein Information
inter-alpha-trypsin inhibitor heavy chain H4
UniProt Protein Name
Inter-alpha-trypsin inhibitor heavy chain H4
UniProt Synonym Protein Names
Inter-alpha-trypsin inhibitor family heavy chain-related protein; IHRP
UniProt Gene Name
ITIH4  [Similar Products]
UniProt Synonym Gene Names
IHRP; ITIHL1; PK120; ITI heavy chain H4; ITI-HC4; Inter-alpha-inhibitor heavy chain 4; IHRP; Gp120; PK-120  [Similar Products]
UniProt Entry Name
ITIH4_HUMAN
NCBI Summary for ITIH4
The protein encoded by this gene is secreted into the blood, where it is cleaved by plasma kallikrein into two smaller forms. Expression of this gene has been detected only in liver, and it seems to be upregulated during surgical trauma. This gene is part of a cluster of similar genes on chromosome 3. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2009]
UniProt Comments for ITIH4
ITIH4: May be involved in acute phase reactions. Belongs to the ITIH family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 3p21.1

Cellular Component: extracellular region; plasma membrane

Molecular Function: endopeptidase inhibitor activity; protein binding; serine-type endopeptidase inhibitor activity

Biological Process: acute-phase response; hyaluronan metabolic process; platelet degranulation; response to cytokine stimulus

Disease: Hypercholesterolemia, Familial
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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