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anti-KPNa2 antibody :: Rabbit anti-Human Karyopherin Alpha 2 (KPNa2) Polyclonal Antibody

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Catalog # MBS2005941 (SPECIAL PROMOTION: Get FREE $5 Starbucks gift card (Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Unit / Price
  0.01 mg  /  $110 +1 FREE 8GB USB
  0.02 mg  /  $130 +1 FREE 8GB USB
  0.05 mg  /  $175 +1 FREE 8GB USB
  0.1 mg  /  $225 +1 FREE 8GB USB
  0.2 mg  /  $340 +1 FREE 8GB USB
  1 mg  /  $800 +1 FREE 8GB USB
Product Name

Karyopherin Alpha 2 (KPNa2), Polyclonal Antibody

Popular Item
Also Known As

Polyclonal Antibody to Karyopherin Alpha 2 (KPNa2)

Matching Pairs
Matching Pairs
Matching Pairs
Matching Pairs
Matching Pairs
Matching Pairs
Matching Pairs
Unconjugated Antibody: Karyopherin Alpha 2 (KPNa2) (MBS2005941)
APC-CY7 Conjugated Secondary Antibody: Immunoglobulin G (MBS2090675)
Matching Pairs
Unconjugated Antibody: Karyopherin Alpha 2 (KPNa2) (MBS2005941)
Unconjugated Secondary Antibody: Immunoglobulin G (MBS2090678)
Matching Pairs
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
529
Immunogen Sequence
Antigen: The target protein is fused with two N-terminal Tags, His-tag and its sequence is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- AVVD GGAIPAFISL LASPHAHISE QAVWALGNIA GDGSVFRDLV IKYGAVDPLL ALLAVPDMSS LACGYLRNLT WTLSNLCRNK NPAPPIDAVE QILPTLVRLL HHDDPEVLAD TCWAISYLTD GPNERIGMVV KTGVVPQLVK LLGASELPIV TPALRAIGNI VTGTDEQTQV VIDAGALAVF PSLLTNPKTN IQKEATWTMS NITAGRQDQI QQVVNHGLVP FLVSVLSKAD FKTQKEAVWA VTNYTSGGTV EQ
OMIM
U28386 mRNA
3D Structure
ModBase 3D Structure for P52292
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Specificity
The antibody is a rabbit polyclonal antibody raised against KPNa2. It has been selected for its ability to recognize KPNa2 in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
Concentration
200ug/ml (lot specific)
Immunogen
Recombinant KPNa2 (Ala157~Gln412) expressed in E.coli.
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2075229
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Other Notes
Small volumes of anti-KPNa2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Product Categories/Family for anti-KPNa2 antibody
Applications Tested/Suitable for anti-KPNa2 antibody
Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
Application Notes for anti-KPNa2 antibody
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200

Western Blot (WB) Knockout Verification of anti-KPNa2 antibody
Knockout Verification: Lane 1: Wild-type HepG2 cell lysate
Lane 2: KPNa2 knockout HepG2 cell lysate
Predicted MW: 58kDa ;Observed MW: 58kDa;Primary Ab: 3ug/ml Rabbit Anti-Human KPNa2 Antibody;Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody;
anti-KPNa2 antibody Western Blot (WB) Knockout Verification (WB) image
Western Blot (WB) of anti-KPNa2 antibody
Western Blot; Lane1: Human HepG2 Cells; Lane2: Human 293T Cells; Lane3: Human Raji Cells.
anti-KPNa2 antibody Western Blot (WB) (WB) image
Western Blot (WB) of anti-KPNa2 antibody
Western Blot; Sample: Recombinant KPNa2, Human.
anti-KPNa2 antibody Western Blot (WB) (WB) image
Immunohistochemistry (IHC) of anti-KPNa2 antibody
DAB staining on IHC-P. Samples: Human Tissue)
anti-KPNa2 antibody Immunohistochemistry (IHC) (IHC) image
NCBI/Uniprot data below describe general gene information for KPNa2. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
57,862 Da
NCBI Official Full Name
importin subunit alpha-1
NCBI Official Synonym Full Names
karyopherin subunit alpha 2
NCBI Official Symbol
KPNA2  [Similar Products]
NCBI Official Synonym Symbols
QIP2; RCH1; IPOA1; SRP1alpha; SRP1-alpha
  [Similar Products]
NCBI Protein Information
importin subunit alpha-1
UniProt Protein Name
Importin subunit alpha-1
UniProt Synonym Protein Names
Karyopherin subunit alpha-2; RAG cohort protein 1; SRP1-alpha
UniProt Gene Name
KPNA2  [Similar Products]
UniProt Synonym Gene Names
RCH1; SRP1  [Similar Products]
NCBI Summary for KPNa2
The import of proteins into the nucleus is a process that involves at least 2 steps. The first is an energy-independent docking of the protein to the nuclear envelope and the second is an energy-dependent translocation through the nuclear pore complex. Imported proteins require a nuclear localization sequence (NLS) which generally consists of a short region of basic amino acids or 2 such regions spaced about 10 amino acids apart. Proteins involved in the first step of nuclear import have been identified in different systems. These include the Xenopus protein importin and its yeast homolog, SRP1 (a suppressor of certain temperature-sensitive mutations of RNA polymerase I in Saccharomyces cerevisiae), which bind to the NLS. KPNA2 protein interacts with the NLSs of DNA helicase Q1 and SV40 T antigen and may be involved in the nuclear transport of proteins. KPNA2 also may play a role in V(D)J recombination. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2016]
UniProt Comments for KPNa2
KPNA2: Functions in nuclear protein import as an adapter protein for nuclear receptor KPNB1. Binds specifically and directly to substrates containing either a simple or bipartite NLS motif. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran- dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Belongs to the importin alpha family.

Protein type: Karyopherin; Nuclear import; Nucleoporin

Chromosomal Location of Human Ortholog: 17q24.2

Cellular Component: cytoplasm; cytosol; membrane; nuclear pore; nucleoplasm

Molecular Function: histone deacetylase binding; nuclear localization sequence binding; protein binding; protein transporter activity; RNA binding

Biological Process: DNA metabolic process; modulation by virus of host process; NLS-bearing substrate import into nucleus; regulation of DNA recombination
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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