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anti-SLC12A6 antibody :: Goat KCC3/SLC12A6 Polyclonal Antibody

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Catalog # MBS420679
Unit / Price
  0.1 mg  /  $300 +1 FREE 8GB USB
Product Name

KCC3/SLC12A6, Polyclonal Antibody

Also Known As

Goat anti-KCC3/SLC12A6 Antibody

Product Synonym Names
SLC12A6; KCC3; solute carrier family 12 (potassium/chloride transporters), member 6; ACCPN; DKFZP434D2135; KCC3A; KCC3B; agenesis of corpus callosum and peripheral neuropathy (Andermann syndrome); potassium chloride cotransporter 3; potassium chloride c; SLC12A6 antibody; KCC3 antibody; solute carrier family 12 (potassium/chloride transporters); member 6 antibody; ACCPN antibody; DKFZP434D2135 antibody; KCC3A antibody; KCC3B antibody; agenesis of corpus callosum and peripheral neuropathy (Andermann syndrome) antibody; potassium chloride cotransporter 3 antibody; potassium chloride c antibody; KCC3 / SLC12A6
Product Synonym Gene Name
Matching Pairs
Unconjugated Antibody: KCC3/SLC12A6 (MBS420679)
Biotin Conjugated Antibody: KCC3 / SLC12A6 (MBS423681)
Antibody/Peptide Pairs
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
1091
Immunogen Sequence
SRHIDVCSKT KE
OMIM
218000
Clonality
Polyclonal
Host
Goat
Species Reactivity
Tested: Human; Expected from sequence similarity: Human
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (lot specific)
Immunogen
Peptide with sequence SRHIDVCSKTKE, from the internal region of the protein sequence according to NP_001035959.1; NP_001035960.1; NP_001035961.1; NP_001035962.1; NP_005126.1; NP_598408.1.
Epitope
Internal region
Note
This antibody is expected to recognise all 6 reported isofoms (NP_598408.1; NP_005126.1; NP_001035959.1; NP_001035960.1; NP_001035961.1; NP_001035962.1;);
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Other Notes
Small volumes of anti-SLC12A6 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-SLC12A6 antibody
Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-SLC12A6 antibody
Peptide ELISA: Antibody detection limit dilution 1: 16000.
Immunohistochemistry: In paraffin embedded Human Heart shows staining of myocardial fibrils in transverse section. Recommended concentration, 2-4ug/ml.
Western Blot: Approx 120kDa band observed in Human Heart and Kidney lysates (calculated MW ranging 121-128kDa according to NP_598408.1; NP_005126.1; NP_001035959.1; NP_001035960.1; NP_001035961.1; NP_001035962.1). Recommended concentration: 0.1-0.3ug/ml.

Western Blot (WB) of anti-SLC12A6 antibody
(0.1ug/ml) staining of Human Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
anti-SLC12A6 antibody Western Blot (WB) (WB) image
Immunohistochemistry (IHC) of anti-SLC12A6 antibody
(2.5ug/ml) staining of paraffin embedded Human Heart. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
anti-SLC12A6 antibody Immunohistochemistry (IHC) (IHC) image
NCBI/Uniprot data below describe general gene information for SLC12A6. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI Related Accession #
Manufactured in an ISO 9001:2015 Certified Laboratory.NP_001035960.1; NP_001035961.1; NP_001035962.1; NP_005126.1; NP_598408.1[Other Products]
NCBI GenBank Nucleotide #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
120,467 Da
NCBI Official Full Name
solute carrier family 12 member 6 isoform c
NCBI Official Synonym Full Names
solute carrier family 12 member 6
NCBI Official Symbol
SLC12A6  [Similar Products]
NCBI Official Synonym Symbols
KCC3; ACCPN; KCC3A; KCC3B
  [Similar Products]
NCBI Protein Information
solute carrier family 12 member 6
UniProt Protein Name
Solute carrier family 12 member 6
UniProt Synonym Protein Names
Electroneutral potassium-chloride cotransporter 3; K-Cl cotransporter 3
Protein Family
UniProt Gene Name
SLC12A6  [Similar Products]
UniProt Synonym Gene Names
UniProt Entry Name
S12A6_HUMAN
NCBI Summary for SLC12A6
This gene is a member of the K-Cl cotransporter (KCC) family. K-Cl cotransporters are integral membrane proteins that lower intracellular chloride concentrations below the electrochemical equilibrium potential. The proteins encoded by this gene are activated by cell swelling induced by hypotonic conditions. Alternate splicing results in multiple transcript variants encoding different isoforms. Mutations in this gene are associated with agenesis of the corpus callosum with peripheral neuropathy. [provided by RefSeq, Jul 2008]
UniProt Comments for SLC12A6
KCC3: Mediates electroneutral potassium-chloride cotransport. May be activated by cell swelling. May contribute to cell volume homeostasis in single cells. Homomultimer and heteromultimer with other K-Cl cotransporters. Up-regulated by VEGF. Down-regulated by TNF. Highly expressed in heart, brain and kidney. Detected at lower levels in skeletal muscle, placenta, lung and pancreas. Detected in umbilical vein endothelial cells. Isoform 2 is more abundant in kidney. Isoform 5 is testis specific. Expressed in the proximal tubule of the kidney. Activated by N-ethylmaleimide (NEM). Inhibited by DIOA, bumetanide and furosemide. Inhibited by WNK3. Belongs to the SLC12A transporter family. 6 isoforms of the human protein are produced by alternative promoter.

Protein type: Transporter, SLC family; Transporter; Membrane protein, integral; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 15q13

Cellular Component: basolateral plasma membrane; integral to membrane; integral to plasma membrane; plasma membrane

Molecular Function: potassium ion symporter activity; potassium ion transmembrane transporter activity; potassium:chloride symporter activity; protein kinase binding

Biological Process: angiogenesis; ion transport; potassium ion import; synaptic transmission

Disease: Agenesis Of The Corpus Callosum With Peripheral Neuropathy
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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