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anti-LAMC2 antibody :: Rabbit anti-Human Laminin Gamma 2 Polyclonal Antibody

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Catalog # MBS2027068 (SPECIAL PROMOTION: Get FREE $5 Starbucks gift card (Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Unit / Price
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  0.01 mg  /  $110 +1 FREE 8GB USB
  0.02 mg  /  $130 +1 FREE 8GB USB
  0.05 mg  /  $175 +1 FREE 8GB USB
  0.1 mg  /  $225 +1 FREE 8GB USB
  0.2 mg  /  $340 +1 FREE 8GB USB
  1 mg  /  $800 +1 FREE 8GB USB
Product Name

Laminin Gamma 2 (LAMC2), Polyclonal Antibody

Popular Item
Full Product Name

Polyclonal Antibody to Laminin Gamma 2 (LAMC2)

Matching Pairs
Matching Pairs
Unconjugated Antibody: Laminin Gamma 2 (MBS2027068)
APC-CY7 Conjugated Antibody: Laminin Gamma 2 (LAMC2) (MBS2062915)
Matching Pairs
Unconjugated Antibody: Laminin Gamma 2 (MBS2027068)
PE Conjugated Antibody: Laminin Gamma 2 (LAMC2) (MBS2062918)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence
Antigen: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- CDC NGKSRQCIFD RELHRQTGNG FRCLNCNDNT DGIHCEKCKN GFYRHRERDR CLPCNCNSKG SLSARCDNSG RCSCKPGVTG ARCDRCLPGF HMLTDAGCTQ DQRLLDSKCD CDPAGIAGPC DAGRCVCKPA VTGERCDRCR SGYYNLDGGN PEGCTQCFCY GHSASC
OMIM
Z15008 mRNA
3D Structure
ModBase 3D Structure for Q13753
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Specificity
The antibody is a rabbit polyclonal antibody raised against LAMC2. It has beenselected for its ability to recognize LAMC2 in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Liquid
Concentration
200ug/ml (lot specific)
Fragment
LAMC2 (Cys28~Cys196)
Organism Species
Homo sapiens (Human)
Conjugate
No Conjugate
Immunogen
Recombinant LAMC2 (Cys28~Cys196) expressed in E Coli.
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2062921
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
ISO Certification
Manufactured in an ISO 9001:2008 and ISO 13485:2003 Certified Laboratory.
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.
Other Notes
Small volumes of anti-LAMC2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Product Categories/Family for anti-LAMC2 antibody
Applications Tested/Suitable for anti-LAMC2 antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA)
Application Notes for anti-LAMC2 antibody
Western blotting: 0.2-2ug/mL;1:250-2500
Immunohistochemistry: 5-20ug/mL;1:25-100
Immunocytochemistry: 5-20ug/mL;1:25-100
Optimal working dilutions must be determined by end user.

Western Blot (WB) of anti-LAMC2 antibody
Western Blot: Sample: Human Lung lysate; Primary Ab: 5ug/ml Rabbit Anti-Human LAMC2 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
anti-LAMC2 antibody Western Blot (WB) (WB) image
Western Blot (WB) of anti-LAMC2 antibody
Western Blot: Sample: Recombinant protein.
anti-LAMC2 antibody Western Blot (WB) (WB) image
Immunohistochemistry (IHC) of anti-LAMC2 antibody
DAB staining on fromalin fixed paraffin-embedded Kidney tissue)
anti-LAMC2 antibody Immunohistochemistry (IHC) (IHC) image
Knockout Validation of anti-LAMC2 antibody
Knockout Validation: Lane 1: Wild-type A431 cell lysate; Lane 2: LAMC2 knockout A431 cell lysate; Predicted MW: 121,130kd Observed MW: 120kd Primary Ab: 5ug/ml Rabbit Anti-Human LAMC2 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (#MBS2086047)
anti-LAMC2 antibody Knockout Validation image
NCBI/Uniprot data below describe general gene information for LAMC2. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
121,604 Da
NCBI Official Full Name
laminin subunit gamma-2 isoform a
NCBI Official Synonym Full Names
laminin subunit gamma 2
NCBI Official Symbol
LAMC2  [Similar Products]
NCBI Official Synonym Symbols
B2T; CSF; EBR2; BM600; EBR2A; LAMB2T; LAMNB2
  [Similar Products]
NCBI Protein Information
laminin subunit gamma-2
UniProt Protein Name
Laminin subunit gamma-2
UniProt Synonym Protein Names
Cell-scattering factor 140 kDa subunit; CSF 140 kDa subunit; Epiligrin subunit gamma; Kalinin subunit gamma; Kalinin/nicein/epiligrin 100 kDa subunit; Ladsin 140 kDa subunit; Laminin B2t chain; Laminin-5 subunit gamma; Large adhesive scatter factor 140 kDa subunit; Nicein subunit gamma
Protein Family
UniProt Gene Name
LAMC2  [Similar Products]
UniProt Synonym Gene Names
LAMB2T; LAMNB2; CSF 140 kDa subunit  [Similar Products]
NCBI Summary for LAMC2
Laminins, a family of extracellular matrix glycoproteins, are the major noncollagenous constituent of basement membranes. They have been implicated in a wide variety of biological processes including cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis. Laminins, composed of 3 non identical chains: laminin alpha, beta and gamma (formerly A, B1, and B2, respectively), have a cruciform structure consisting of 3 short arms, each formed by a different chain, and a long arm composed of all 3 chains. Each laminin chain is a multidomain protein encoded by a distinct gene. Several isoforms of each chain have been described. Different alpha, beta and gamma chain isomers combine to give rise to different heterotrimeric laminin isoforms which are designated by Arabic numerals in the order of their discovery, i.e. alpha1beta1gamma1 heterotrimer is laminin 1. The biological functions of the different chains and trimer molecules are largely unknown, but some of the chains have been shown to differ with respect to their tissue distribution, presumably reflecting diverse functions in vivo. This gene encodes the gamma chain isoform laminin, gamma 2. The gamma 2 chain, formerly thought to be a truncated version of beta chain (B2t), is highly homologous to the gamma 1 chain; however, it lacks domain VI, and domains V, IV and III are shorter. It is expressed in several fetal tissues but differently from gamma 1, and is specifically localized to epithelial cells in skin, lung and kidney. The gamma 2 chain together with alpha 3 and beta 3 chains constitute laminin 5 (earlier known as kalinin), which is an integral part of the anchoring filaments that connect epithelial cells to the underlying basement membrane. The epithelium-specific expression of the gamma 2 chain implied its role as an epithelium attachment molecule, and mutations in this gene have been associated with junctional epidermolysis bullosa, a skin disease characterized by blisters due to disruption of the epidermal-dermal junction. Two transcript variants resulting from alternative splicing of the 3' terminal exon, and encoding different isoforms of gamma 2 chain, have been described. The two variants are differentially expressed in embryonic tissues, however, the biological significance of the two forms is not known. Transcript variants utilizing alternative polyA_signal have also been noted in literature. [provided by RefSeq, Aug 2011]
UniProt Comments for LAMC2
LAMC2: Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. Ladsin exerts cell- scattering activity toward a wide variety of cells, including epithelial, endothelial, and fibroblastic cells. Defects in LAMC2 are a cause of epidermolysis bullosa junctional Herlitz type (H-JEB); also known as junctional epidermolysis bullosa Herlitz-Pearson type. JEB defines a group of blistering skin diseases characterized by tissue separation which occurs within the dermo-epidermal basement membrane. H-JEB is a severe, infantile and lethal form. Death occurs usually within the first six months of life. Occasionally, children survive to teens. H-JEB is marked by bullous lesions at birth and extensive denudation of skin and mucous membranes that may be hemorrhagic. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Extracellular matrix; Motility/polarity/chemotaxis; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 1q25.3

Cellular Component: cell cortex; extracellular region; extracellular space; laminin-2 complex; membrane; perinuclear region of cytoplasm

Molecular Function: heparin binding

Biological Process: cell adhesion; epidermis development; extracellular matrix disassembly; extracellular matrix organization; hemidesmosome assembly; positive regulation of cell migration; positive regulation of cell proliferation; system development

Disease: Epidermolysis Bullosa, Junctional, Herlitz Type; Epidermolysis Bullosa, Junctional, Non-herlitz Type
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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